TY - JOUR T1 - A malectin-like/leucine-rich repeat receptor protein kinase gene, RLK-V, regulates powdery mildew resistance in wheat. AU - Hu,Ping, AU - Liu,Jiaqian, AU - Xu,Jiefei, AU - Zhou,Chuanyu, AU - Cao,Shuqi, AU - Zhou,Weihao, AU - Huang,Zhenpu, AU - Yuan,Sufan, AU - Wang,Xiue, AU - Xiao,Jin, AU - Zhang,Ruiqi, AU - Wang,Haiyan, AU - Zhang,Shouzhong, AU - Xing,Liping, AU - Cao,Aizhong, Y1 - 2018/10/16/ PY - 2018/04/29/received PY - 2018/07/16/revised PY - 2018/07/18/accepted PY - 2018/7/22/pubmed PY - 2019/7/30/medline PY - 2018/7/22/entrez KW - RLK-V KW - disease resistance KW - malectin-like/leucine-rich repeat receptor protein kinase KW - powdery mildew KW - wheat. SP - 2561 EP - 2574 JF - Molecular plant pathology JO - Mol Plant Pathol VL - 19 IS - 12 N2 - Pattern recognition receptors (PRRs) can trigger plant immunity through the recognition of pathogen-associated molecular patterns. In this study, we report that a malectin-like/leucine-rich repeat receptor protein kinase gene, RLK-V, from Haynaldia villosa putatively acts as a PRR to positively regulate powdery mildew resistance caused by Blumeria graminis f. sp. tritici (Bgt) in wheat. RLK-V has two alternatively spliced transcripts corresponding to an intact RLK-V1.1 and a truncated RLK-V1.2 caused by intron retention. Expression analysis showed that both transcripts could be up-regulated by Bgt in resistant materials, whereas the functional RLK-V1.1 was expressed only after Bgt inoculation. Promoter activity assays indicated that RLK-V could respond to Bgt even in susceptible wheat. Silencing of RLK-V in Pm21-carrying resistant materials resulted in compromised resistance to Bgt. In addition, over-expression of RLK-V1.1 in Pm21-lacking susceptible Yangmai158 and SM-1 by single-cell transient expression and stable transformation in Yangmai158 could improve powdery mildew resistance. We propose that RLK-V regulates basal resistance to powdery mildew, which is also required for broad-spectrum resistance mediated by the Pm21 gene. Over-expression of RLK-V1.1 could trigger cell death in Nicotiana benthamiana, and RLK-V1.1 transgenic wheat accumulated more reactive oxygen species and displayed a stronger hypersensitive response than did the recipient, which led to enhanced Bgt resistance. However, constitutive activation of RLK-V1.1 resulted in the abnormal growth of transgenic plants. SN - 1364-3703 UR - https://www.unboundmedicine.com/medline/citation/30030900/A_malectin_like/leucine_rich_repeat_receptor_protein_kinase_gene_RLK_V_regulates_powdery_mildew_resistance_in_wheat_ DB - PRIME DP - Unbound Medicine ER -