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A rapid method to determine nine natural cannabinoids in beverages and food derived from Cannabis sativa by liquid chromatography coupled to tandem mass spectrometry on a QTRAP 4000.
Rapid Commun Mass Spectrom 2018; 32(19):1728-1736RC

Abstract

RATIONALE

Phytocannabinoids are natural compounds produced by Cannabis spp. Some of these compounds show psychotropic effects on humans and are therefore used as drugs of abuse. These compounds are present in food and beverages containing ingredients from hemp, and thus can reach consumers. The Italian Ministry of Health planned to evaluate the intake of cannabinoids from food containing hemp ingredients. Thus, we were asked to develop and validate a multi-residue test method to determine nine phytocannabinoids in beverages and food.

METHODS

Nine natural phytocannabinoids, hereafter called cannabinoids, were cleaned up from food by solid-liquid extraction, while beverages were simply diluted prior to analysis. The cannabinoids were separated by reversed-phase high-performance liquid chromatography, and on-line determination was carried out by tandem mass spectrometry using a 4000 QTRAP mass spectrometer with a TurboIonSpray source, in multiple-reaction monitoring mode, using both positive and negative ionization.

RESULTS

Each compound was determined down to 0.25 ng/mL in solvent. In-house validation was carried out; the mean recoveries ranged from 83.4% to 101.2% in food, and from 84.5% to 104.5% in beverages. The limits of quantification were 20 μg/kg for food and 2 μg/L for beverages.

CONCLUSIONS

A reliable and rapid method for the identification and quantification of the psychotropic Δ9 -tetrahydrocannabinol, its non-psychoactive precursor Δ9 -tetrahydrocannabinolic acid A, and seven other cannabinoids was developed and validated, to monitor the content of these substances in food and beverages produced using hemp seeds, flour and oil as ingredients.

Authors+Show Affiliations

Department of Chemistry, Istituto Zooprofilattico Sperimentale del Mezzogiorno, via Salute 2, Portici, NA, 80055, Italy.Department of Chemistry, Istituto Zooprofilattico Sperimentale del Mezzogiorno, via Salute 2, Portici, NA, 80055, Italy.Department of Chemistry, Istituto Zooprofilattico Sperimentale del Mezzogiorno, via Salute 2, Portici, NA, 80055, Italy.Department of Chemistry, Istituto Zooprofilattico Sperimentale del Mezzogiorno, via Salute 2, Portici, NA, 80055, Italy.Department of Chemistry, Istituto Zooprofilattico Sperimentale del Mezzogiorno, via Salute 2, Portici, NA, 80055, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30030940

Citation

Di Marco Pisciottano, Ilaria, et al. "A Rapid Method to Determine Nine Natural Cannabinoids in Beverages and Food Derived From Cannabis Sativa By Liquid Chromatography Coupled to Tandem Mass Spectrometry On a QTRAP 4000." Rapid Communications in Mass Spectrometry : RCM, vol. 32, no. 19, 2018, pp. 1728-1736.
Di Marco Pisciottano I, Guadagnuolo G, Soprano V, et al. A rapid method to determine nine natural cannabinoids in beverages and food derived from Cannabis sativa by liquid chromatography coupled to tandem mass spectrometry on a QTRAP 4000. Rapid Commun Mass Spectrom. 2018;32(19):1728-1736.
Di Marco Pisciottano, I., Guadagnuolo, G., Soprano, V., De Crescenzo, M., & Gallo, P. (2018). A rapid method to determine nine natural cannabinoids in beverages and food derived from Cannabis sativa by liquid chromatography coupled to tandem mass spectrometry on a QTRAP 4000. Rapid Communications in Mass Spectrometry : RCM, 32(19), pp. 1728-1736. doi:10.1002/rcm.8242.
Di Marco Pisciottano I, et al. A Rapid Method to Determine Nine Natural Cannabinoids in Beverages and Food Derived From Cannabis Sativa By Liquid Chromatography Coupled to Tandem Mass Spectrometry On a QTRAP 4000. Rapid Commun Mass Spectrom. 2018 Oct 15;32(19):1728-1736. PubMed PMID: 30030940.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A rapid method to determine nine natural cannabinoids in beverages and food derived from Cannabis sativa by liquid chromatography coupled to tandem mass spectrometry on a QTRAP 4000. AU - Di Marco Pisciottano,Ilaria, AU - Guadagnuolo,Grazia, AU - Soprano,Vittorio, AU - De Crescenzo,Michele, AU - Gallo,Pasquale, PY - 2018/02/23/received PY - 2018/07/12/revised PY - 2018/07/14/accepted PY - 2018/7/22/pubmed PY - 2018/11/21/medline PY - 2018/7/22/entrez SP - 1728 EP - 1736 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun. Mass Spectrom. VL - 32 IS - 19 N2 - RATIONALE: Phytocannabinoids are natural compounds produced by Cannabis spp. Some of these compounds show psychotropic effects on humans and are therefore used as drugs of abuse. These compounds are present in food and beverages containing ingredients from hemp, and thus can reach consumers. The Italian Ministry of Health planned to evaluate the intake of cannabinoids from food containing hemp ingredients. Thus, we were asked to develop and validate a multi-residue test method to determine nine phytocannabinoids in beverages and food. METHODS: Nine natural phytocannabinoids, hereafter called cannabinoids, were cleaned up from food by solid-liquid extraction, while beverages were simply diluted prior to analysis. The cannabinoids were separated by reversed-phase high-performance liquid chromatography, and on-line determination was carried out by tandem mass spectrometry using a 4000 QTRAP mass spectrometer with a TurboIonSpray source, in multiple-reaction monitoring mode, using both positive and negative ionization. RESULTS: Each compound was determined down to 0.25 ng/mL in solvent. In-house validation was carried out; the mean recoveries ranged from 83.4% to 101.2% in food, and from 84.5% to 104.5% in beverages. The limits of quantification were 20 μg/kg for food and 2 μg/L for beverages. CONCLUSIONS: A reliable and rapid method for the identification and quantification of the psychotropic Δ9 -tetrahydrocannabinol, its non-psychoactive precursor Δ9 -tetrahydrocannabinolic acid A, and seven other cannabinoids was developed and validated, to monitor the content of these substances in food and beverages produced using hemp seeds, flour and oil as ingredients. SN - 1097-0231 UR - https://www.unboundmedicine.com/medline/citation/30030940/A_rapid_method_to_determine_nine_natural_cannabinoids_in_beverages_and_food_derived_from_Cannabis_sativa_by_liquid_chromatography_coupled_to_tandem_mass_spectrometry_on_a_QTRAP_4000_ DB - PRIME DP - Unbound Medicine ER -