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Muscle-specific activation of calpain system in hindlimb unloading rats and hibernating Daurian ground squirrels: a comparison between artificial and natural disuse.

Abstract

To determine whether the regulation of calpain system is involved in non-hibernators and hibernators in disused condition, the soleus (SOL) and extensor digitorum longus (EDL) muscles were used for investigating the muscle mass, the ratio of muscle wet weight/body weight (MWW/BW), fiber-type distribution, fiber cross-sectional area (CSA), and the protein expression of MuRF1, calpain-1, calpain-2, calpastatin, desmin, troponin T, and troponin C in hindlimb unloading rats and hibernating Daurian ground squirrels. The muscle mass, MWW/BW, and fiber CSA were found significantly decreased in SOL and EDL of hindlimb unloading rats, but unchanged in hibernating ground squirrels. The MuRF1 expression was increased in both SOL and EDL of unloading rats, while it was only increased in SOL, but maintained in EDL of hibernating ground squirrels. The expression levels of calpain-1 and calpain-2 were increased in different degrees in unloaded SOL and EDL in rats, while they were maintained in EDL and even reduced in SOL of hibernating ground squirrels. Besides, the expression of calpastatin was decreased in unloaded rats, but increased in hibernating ground squirrels. The desmin expression was decreased in unloaded rats, but maintained in hibernating squirrels. Interestingly, the levels of troponin T and troponin C were decreased in both SOL and EDL of unloaded rats, but increased in hibernating ground squirrels with muscle-type specificity. In conclusion, differential calpain activation and substrate-selective degradation in slow and fast muscles are involved in the mechanisms of muscle atrophy of unloaded rats and remarkable ability of muscle maintenance of hibernating ground squirrels.

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  • Authors+Show Affiliations

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    Shaanxi Key Laboratory for Animal Conservation, Northwest University, Xi'an, 710069, People's Republic of China. Key Laboratory of Resource Biology and Biotechnology in Western China, College of Life Sciences, Ministry of Education, Northwest University, 229# North Taibai Road, Xi'an, 710069, People's Republic of China.

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    Key Laboratory of Resource Biology and Biotechnology in Western China, College of Life Sciences, Ministry of Education, Northwest University, 229# North Taibai Road, Xi'an, 710069, People's Republic of China.

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    Key Laboratory of Resource Biology and Biotechnology in Western China, College of Life Sciences, Ministry of Education, Northwest University, 229# North Taibai Road, Xi'an, 710069, People's Republic of China.

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    Key Laboratory of Resource Biology and Biotechnology in Western China, College of Life Sciences, Ministry of Education, Northwest University, 229# North Taibai Road, Xi'an, 710069, People's Republic of China.

    ,

    Shaanxi Key Laboratory for Animal Conservation, Northwest University, Xi'an, 710069, People's Republic of China. Key Laboratory of Resource Biology and Biotechnology in Western China, College of Life Sciences, Ministry of Education, Northwest University, 229# North Taibai Road, Xi'an, 710069, People's Republic of China.

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    Key Laboratory of Resource Biology and Biotechnology in Western China, College of Life Sciences, Ministry of Education, Northwest University, 229# North Taibai Road, Xi'an, 710069, People's Republic of China.

    Shaanxi Key Laboratory for Animal Conservation, Northwest University, Xi'an, 710069, People's Republic of China. gaoyunf@nwu.edu.cn. Key Laboratory of Resource Biology and Biotechnology in Western China, College of Life Sciences, Ministry of Education, Northwest University, 229# North Taibai Road, Xi'an, 710069, People's Republic of China. gaoyunf@nwu.edu.cn.

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    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    30039299

    Citation

    Chang, Hui, et al. "Muscle-specific Activation of Calpain System in Hindlimb Unloading Rats and Hibernating Daurian Ground Squirrels: a Comparison Between Artificial and Natural Disuse." Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology, vol. 188, no. 5, 2018, pp. 863-876.
    Chang H, Lei T, Ma X, et al. Muscle-specific activation of calpain system in hindlimb unloading rats and hibernating Daurian ground squirrels: a comparison between artificial and natural disuse. J Comp Physiol B, Biochem Syst Environ Physiol. 2018;188(5):863-876.
    Chang, H., Lei, T., Ma, X., Zhang, J., Wang, H., Zhang, X., & Gao, Y. F. (2018). Muscle-specific activation of calpain system in hindlimb unloading rats and hibernating Daurian ground squirrels: a comparison between artificial and natural disuse. Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology, 188(5), pp. 863-876. doi:10.1007/s00360-018-1176-z.
    Chang H, et al. Muscle-specific Activation of Calpain System in Hindlimb Unloading Rats and Hibernating Daurian Ground Squirrels: a Comparison Between Artificial and Natural Disuse. J Comp Physiol B, Biochem Syst Environ Physiol. 2018;188(5):863-876. PubMed PMID: 30039299.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Muscle-specific activation of calpain system in hindlimb unloading rats and hibernating Daurian ground squirrels: a comparison between artificial and natural disuse. AU - Chang,Hui, AU - Lei,Tingyun, AU - Ma,Xiufeng, AU - Zhang,Jie, AU - Wang,Huiping, AU - Zhang,Xingyuan, AU - Gao,Yun-Fang, Y1 - 2018/07/23/ PY - 2018/04/06/received PY - 2018/07/17/accepted PY - 2018/06/14/revised PY - 2018/7/25/pubmed PY - 2018/7/25/medline PY - 2018/7/25/entrez KW - Calpain KW - Disuse muscle atrophy KW - Hibernation KW - Hindlimb unloading SP - 863 EP - 876 JF - Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology JO - J. Comp. Physiol. B, Biochem. Syst. Environ. Physiol. VL - 188 IS - 5 N2 - To determine whether the regulation of calpain system is involved in non-hibernators and hibernators in disused condition, the soleus (SOL) and extensor digitorum longus (EDL) muscles were used for investigating the muscle mass, the ratio of muscle wet weight/body weight (MWW/BW), fiber-type distribution, fiber cross-sectional area (CSA), and the protein expression of MuRF1, calpain-1, calpain-2, calpastatin, desmin, troponin T, and troponin C in hindlimb unloading rats and hibernating Daurian ground squirrels. The muscle mass, MWW/BW, and fiber CSA were found significantly decreased in SOL and EDL of hindlimb unloading rats, but unchanged in hibernating ground squirrels. The MuRF1 expression was increased in both SOL and EDL of unloading rats, while it was only increased in SOL, but maintained in EDL of hibernating ground squirrels. The expression levels of calpain-1 and calpain-2 were increased in different degrees in unloaded SOL and EDL in rats, while they were maintained in EDL and even reduced in SOL of hibernating ground squirrels. Besides, the expression of calpastatin was decreased in unloaded rats, but increased in hibernating ground squirrels. The desmin expression was decreased in unloaded rats, but maintained in hibernating squirrels. Interestingly, the levels of troponin T and troponin C were decreased in both SOL and EDL of unloaded rats, but increased in hibernating ground squirrels with muscle-type specificity. In conclusion, differential calpain activation and substrate-selective degradation in slow and fast muscles are involved in the mechanisms of muscle atrophy of unloaded rats and remarkable ability of muscle maintenance of hibernating ground squirrels. SN - 1432-136X UR - https://www.unboundmedicine.com/medline/citation/30039299/Muscle_specific_activation_of_calpain_system_in_hindlimb_unloading_rats_and_hibernating_Daurian_ground_squirrels:_a_comparison_between_artificial_and_natural_disuse_ L2 - http://dx.doi.org/10.1007/s00360-018-1176-z DB - PRIME DP - Unbound Medicine ER -