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Method for detecting viruses in aerosols.
Appl Environ Microbiol 1985; 50(5):1181-6AE

Abstract

A simple method with poliovirus as the model was developed for recovering human enteric viruses from aerosols. Filterite filters (pore size, 0.45 micron; Filterite Corp., Timonium, Md.) moistened with glycine buffer (pH 3.5) were used for adsorbing the aerosolized virus. No virus passed the filter, even with air flow rates of 100 liters/min. Virus recovery from the filter was achieved by rapid elution with 800 ml of glycine buffer, pH 10. The virus in the primary eluate was reconcentrated by adjusting the pH to 3.5, adding AlCl3 to 0.0005 M, collecting the virus on a 0.25-micron-pore Filerite disk (diameter, 25 mm) and and eluting with 6 ml of buffer, pH 10. With this method, virus could be detected regularly in aerosols produced by flushing when 3 X 10(8) PFU of poliovirus were present in the toilet bowl. Poliovirus-containing fecal material from two of four infants who had recently received oral polio vaccine also yielded virus in the aerosols when feces containing 2.4 X 10(7) to 4.5 X 10(7) PFU of virus had been added to the toilet bowl. Persons infected with a variety of natural enteric viruses are known to excrete this amount of virus in their daily stools.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

3004329

Citation

Wallis, C, et al. "Method for Detecting Viruses in Aerosols." Applied and Environmental Microbiology, vol. 50, no. 5, 1985, pp. 1181-6.
Wallis C, Melnick JL, Rao VC, et al. Method for detecting viruses in aerosols. Appl Environ Microbiol. 1985;50(5):1181-6.
Wallis, C., Melnick, J. L., Rao, V. C., & Sox, T. E. (1985). Method for detecting viruses in aerosols. Applied and Environmental Microbiology, 50(5), pp. 1181-6.
Wallis C, et al. Method for Detecting Viruses in Aerosols. Appl Environ Microbiol. 1985;50(5):1181-6. PubMed PMID: 3004329.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Method for detecting viruses in aerosols. AU - Wallis,C, AU - Melnick,J L, AU - Rao,V C, AU - Sox,T E, PY - 1985/11/1/pubmed PY - 1985/11/1/medline PY - 1985/11/1/entrez SP - 1181 EP - 6 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 50 IS - 5 N2 - A simple method with poliovirus as the model was developed for recovering human enteric viruses from aerosols. Filterite filters (pore size, 0.45 micron; Filterite Corp., Timonium, Md.) moistened with glycine buffer (pH 3.5) were used for adsorbing the aerosolized virus. No virus passed the filter, even with air flow rates of 100 liters/min. Virus recovery from the filter was achieved by rapid elution with 800 ml of glycine buffer, pH 10. The virus in the primary eluate was reconcentrated by adjusting the pH to 3.5, adding AlCl3 to 0.0005 M, collecting the virus on a 0.25-micron-pore Filerite disk (diameter, 25 mm) and and eluting with 6 ml of buffer, pH 10. With this method, virus could be detected regularly in aerosols produced by flushing when 3 X 10(8) PFU of poliovirus were present in the toilet bowl. Poliovirus-containing fecal material from two of four infants who had recently received oral polio vaccine also yielded virus in the aerosols when feces containing 2.4 X 10(7) to 4.5 X 10(7) PFU of virus had been added to the toilet bowl. Persons infected with a variety of natural enteric viruses are known to excrete this amount of virus in their daily stools. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/3004329/Method_for_detecting_viruses_in_aerosols_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=3004329 DB - PRIME DP - Unbound Medicine ER -