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Protective Effects of a Polyphenol-Rich Extract from Syzygium cumini (L.) Skeels Leaf on Oxidative Stress-Induced Diabetic Rats.
Oxid Med Cell Longev. 2018; 2018:5386079.OM

Abstract

Syzygium cumini (L.) Skeels has been reported to exert anti-inflammatory and cardiometabolic activities due to its high content of polyphenols. We characterized the chemical composition and assessed the antidiabetic effects of a novel polyphenol-rich extract (PESc) obtained from S. cumini leaf. Rats were injected with alloxan (150 mg/kg, ip, ALX group) and followed up for 7 days. Some were orally treated with PESc (50 mg/kg/day) for 7 days before and after diabetes induction (ALX-PP) or only for 7 days after alloxan injection (ALX-P). ALX-P and ALX-PP decreased fasting glycemia in 37 and 43%, respectively, as compared to ALX. Triglycerides and total cholesterol serum levels were also significantly reduced in comparison to ALX. PESc presented high polyphenol concentration (71.78 ± 8.57 GAE/100 g), with flavonoid content of 8.21 ± 0.42 QE/100 g. Upon HPLC-MS/MS and MS/MS studies, five main polyphenols-gallic acid, quercetin, myricetin, and its derivatives-were identified. Myricetin was predominant (192.70 ± 16.50 μg/mg PESc), followed by measurable amounts of gallic acid (11.15 ± 0.90 μg/mg PESc) and quercetin (4.72 ± 0.06 μg/mg PESc). Kinetic assessment of total antioxidant capacity revealed PESc high potency, since maximum response was reached within 5 min reaction time in a concentration-dependent manner. Specific antioxidant activity of PESc was assessed against both DPPH• and ABTS•+, showing strong activity (IC50: 3.88 ± 1.09 and 5.98 ± 1.19 μg/mL, resp.). PESc also inhibited lipoxygenase activity (IC50: 27.63 ± 8.47), confirming its antioxidant activity also on biologically relevant radicals. Finally, PESc induced insulin secretion by directly stimulating INS-1E β cells in the absence of any cytotoxic effect. Overall, our results support that PESc is a potent antioxidant phytocomplex with potential pharmacological use as a preventive antidiabetic natural product.

Authors+Show Affiliations

Department of Physiological Sciences, Federal University of Maranhão, 65080805 São Luís, MA, Brazil.Department of Physiological Sciences, Federal University of Maranhão, 65080805 São Luís, MA, Brazil.Department of Physiological Sciences, Federal University of Maranhão, 65080805 São Luís, MA, Brazil.Department of Physiological Sciences, Federal University of Maranhão, 65080805 São Luís, MA, Brazil.Department of Biochemistry and Center for Free Radical and Biomedical Research, Faculty of Medicine, University de la República, 11800 Montevideo, Uruguay.Department of Chemistry, Federal University of Maranhão, 65080805 São Luís, MA, Brazil.Department of Pharmacy, Federal University of Maranhão, 65080805 São Luís, MA, Brazil.Department of Physiological Sciences, Federal University of Maranhão, 65080805 São Luís, MA, Brazil.Department of Biochemistry and Center for Free Radical and Biomedical Research, Faculty of Medicine, University de la República, 11800 Montevideo, Uruguay.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30046378

Citation

Chagas, Vinicyus Teles, et al. "Protective Effects of a Polyphenol-Rich Extract From Syzygium Cumini (L.) Skeels Leaf On Oxidative Stress-Induced Diabetic Rats." Oxidative Medicine and Cellular Longevity, vol. 2018, 2018, p. 5386079.
Chagas VT, Coelho RMRS, Gaspar RS, et al. Protective Effects of a Polyphenol-Rich Extract from Syzygium cumini (L.) Skeels Leaf on Oxidative Stress-Induced Diabetic Rats. Oxid Med Cell Longev. 2018;2018:5386079.
Chagas, V. T., Coelho, R. M. R. S., Gaspar, R. S., da Silva, S. A., Mastrogiovanni, M., Mendonça, C. J., Ribeiro, M. N. S., Paes, A. M. A., & Trostchansky, A. (2018). Protective Effects of a Polyphenol-Rich Extract from Syzygium cumini (L.) Skeels Leaf on Oxidative Stress-Induced Diabetic Rats. Oxidative Medicine and Cellular Longevity, 2018, 5386079. https://doi.org/10.1155/2018/5386079
Chagas VT, et al. Protective Effects of a Polyphenol-Rich Extract From Syzygium Cumini (L.) Skeels Leaf On Oxidative Stress-Induced Diabetic Rats. Oxid Med Cell Longev. 2018;2018:5386079. PubMed PMID: 30046378.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective Effects of a Polyphenol-Rich Extract from Syzygium cumini (L.) Skeels Leaf on Oxidative Stress-Induced Diabetic Rats. AU - Chagas,Vinicyus Teles, AU - Coelho,Rafaella Moraes Rego de Sousa, AU - Gaspar,Renato Simões, AU - da Silva,Samira Abdalla, AU - Mastrogiovanni,Mauricio, AU - Mendonça,Cáritas de Jesus, AU - Ribeiro,Maria Nilce de Souza, AU - Paes,Antonio Marcus de Andrade, AU - Trostchansky,Andres, Y1 - 2018/06/26/ PY - 2017/12/28/received PY - 2018/04/05/revised PY - 2018/04/19/accepted PY - 2018/7/27/entrez PY - 2018/7/27/pubmed PY - 2018/10/23/medline SP - 5386079 EP - 5386079 JF - Oxidative medicine and cellular longevity JO - Oxid Med Cell Longev VL - 2018 N2 - Syzygium cumini (L.) Skeels has been reported to exert anti-inflammatory and cardiometabolic activities due to its high content of polyphenols. We characterized the chemical composition and assessed the antidiabetic effects of a novel polyphenol-rich extract (PESc) obtained from S. cumini leaf. Rats were injected with alloxan (150 mg/kg, ip, ALX group) and followed up for 7 days. Some were orally treated with PESc (50 mg/kg/day) for 7 days before and after diabetes induction (ALX-PP) or only for 7 days after alloxan injection (ALX-P). ALX-P and ALX-PP decreased fasting glycemia in 37 and 43%, respectively, as compared to ALX. Triglycerides and total cholesterol serum levels were also significantly reduced in comparison to ALX. PESc presented high polyphenol concentration (71.78 ± 8.57 GAE/100 g), with flavonoid content of 8.21 ± 0.42 QE/100 g. Upon HPLC-MS/MS and MS/MS studies, five main polyphenols-gallic acid, quercetin, myricetin, and its derivatives-were identified. Myricetin was predominant (192.70 ± 16.50 μg/mg PESc), followed by measurable amounts of gallic acid (11.15 ± 0.90 μg/mg PESc) and quercetin (4.72 ± 0.06 μg/mg PESc). Kinetic assessment of total antioxidant capacity revealed PESc high potency, since maximum response was reached within 5 min reaction time in a concentration-dependent manner. Specific antioxidant activity of PESc was assessed against both DPPH• and ABTS•+, showing strong activity (IC50: 3.88 ± 1.09 and 5.98 ± 1.19 μg/mL, resp.). PESc also inhibited lipoxygenase activity (IC50: 27.63 ± 8.47), confirming its antioxidant activity also on biologically relevant radicals. Finally, PESc induced insulin secretion by directly stimulating INS-1E β cells in the absence of any cytotoxic effect. Overall, our results support that PESc is a potent antioxidant phytocomplex with potential pharmacological use as a preventive antidiabetic natural product. SN - 1942-0994 UR - https://www.unboundmedicine.com/medline/citation/30046378/Protective_Effects_of_a_Polyphenol_Rich_Extract_from_Syzygium_cumini__L___Skeels_Leaf_on_Oxidative_Stress_Induced_Diabetic_Rats_ L2 - https://doi.org/10.1155/2018/5386079 DB - PRIME DP - Unbound Medicine ER -