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Quantification of ondansetron, granisetron and tropisetron in goat plasma using hydrophilic interaction liquid chromatography-solid phase extraction coupled with hydrophilic interaction liquid chromatography-triple quadrupole tandem mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Sep 15; 1095:50-58.JC

Abstract

An assay method to quantify ondansetron (OND), granisetron (GRA) and tropisetron (TRO) in goat plasma has been successfully developed and validated. This method procedure for the analysis of OND, GRA and TRO was involved of extracting samples with hydrophilic interaction liquid chromatography (HILIC) solid phase extraction (SPE) and determination by liquid chromatography coupled to tandem mass spectroscopy. An SPE method for the simultaneous extraction of OND, GRA and TRO with high efficiency and selectivity was developed. Prior to HPLC-MS/MS analysis, most of the sources of interference present in the supernatant after protein precipitation of plasma proteins was efficiently removed from the samples by the HILIC SPE treatment. For the quantification of OND, GRA and TRO in the samples, tandem mass spectrometry operating in positive electrospray ionization mode with multiple reaction monitoring was used. The calibration curve was performed in the range of 0.2-20 ng/mL for the target OND, GRA and TRO in goat plasma samples. The precision of the intra- and inter-day assay for OND, GRA and TRO were 1.84-6.23% and 3.89-5.31%, 2.63-6.29% and 3.76-5.31%, 1.99-5.67% and 2.64-4.70%, respectively. The accuracy of the intra- and inter-day assay for OND, GRA and TRO were 89.15-97.39% and 89.46-95.17%, 91.08-100.82% and 91.24-99.47%, 92.30-100.74% and 94.21-97.90%, respectively. For the determination of OND, GRA and TRO in plasma samples, no significant matrix effects were observed. The mean absolute recoveries were 103-150%, 115-121%, and 98-141% for OND, GRA and TRO, respectively. Furthermore, the mean process efficiency values of silica SPE were 98-135%, 92-124%, and 72-109% for OND, GRA and TRO, respectively.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Huang C
Faculty of Chemical, Environmental and Biological Science and Technology, Analytical Center, Dalian University of Technoloy, Dalian 116024, China.
Wan H
Faculty of Chemical, Environmental and Biological Science and Technology, Analytical Center, Dalian University of Technoloy, Dalian 116024, China. Electronic address: wanhuihui@dlut.edu.cn.
Zhang J
Faculty of Chemical, Environmental and Biological Science and Technology, Analytical Center, Dalian University of Technoloy, Dalian 116024, China.
Zhong H
Faculty of Chemical, Environmental and Biological Science and Technology, Analytical Center, Dalian University of Technoloy, Dalian 116024, China.
Li J
Department of Radiation Oncology, Second Affiliated Hospital of Dalian Medical University, Dalian 116027, China.
Sun Y
Faculty of Chemical, Environmental and Biological Science and Technology, Analytical Center, Dalian University of Technoloy, Dalian 116024, China.
Wang Q
School of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian 116024, China.
Zhang H
Faculty of Chemical, Environmental and Biological Science and Technology, Analytical Center, Dalian University of Technoloy, Dalian 116024, China. Electronic address: zhanghua@dlut.edu.cn.

MeSH

AcetonitrilesAnimalsChromatography, LiquidFormatesGoatsGranisetronHydrophobic and Hydrophilic InteractionsIndolesLinear ModelsOndansetronReproducibility of ResultsSensitivity and SpecificitySolid Phase ExtractionTandem Mass SpectrometryTropisetron

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30053687

Citation

Huang, Cunying, et al. "Quantification of Ondansetron, Granisetron and Tropisetron in Goat Plasma Using Hydrophilic Interaction Liquid Chromatography-solid Phase Extraction Coupled With Hydrophilic Interaction Liquid Chromatography-triple Quadrupole Tandem Mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 1095, 2018, pp. 50-58.
Huang C, Wan H, Zhang J, et al. Quantification of ondansetron, granisetron and tropisetron in goat plasma using hydrophilic interaction liquid chromatography-solid phase extraction coupled with hydrophilic interaction liquid chromatography-triple quadrupole tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2018;1095:50-58.
Huang, C., Wan, H., Zhang, J., Zhong, H., Li, J., Sun, Y., Wang, Q., & Zhang, H. (2018). Quantification of ondansetron, granisetron and tropisetron in goat plasma using hydrophilic interaction liquid chromatography-solid phase extraction coupled with hydrophilic interaction liquid chromatography-triple quadrupole tandem mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 1095, 50-58. https://doi.org/10.1016/j.jchromb.2018.07.009
Huang C, et al. Quantification of Ondansetron, Granisetron and Tropisetron in Goat Plasma Using Hydrophilic Interaction Liquid Chromatography-solid Phase Extraction Coupled With Hydrophilic Interaction Liquid Chromatography-triple Quadrupole Tandem Mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Sep 15;1095:50-58. PubMed PMID: 30053687.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantification of ondansetron, granisetron and tropisetron in goat plasma using hydrophilic interaction liquid chromatography-solid phase extraction coupled with hydrophilic interaction liquid chromatography-triple quadrupole tandem mass spectrometry. AU - Huang,Cunying, AU - Wan,Huihui, AU - Zhang,Jing, AU - Zhong,Hongmin, AU - Li,Juan, AU - Sun,YuMing, AU - Wang,Qing, AU - Zhang,Hua, Y1 - 2018/07/11/ PY - 2018/03/09/received PY - 2018/05/23/revised PY - 2018/07/10/accepted PY - 2018/7/28/pubmed PY - 2018/10/10/medline PY - 2018/7/28/entrez KW - Granisetron KW - HILIC-MS/MS KW - HILIC-SPE KW - Ondansetron KW - Plasma KW - Tropisetron SP - 50 EP - 58 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 1095 N2 - An assay method to quantify ondansetron (OND), granisetron (GRA) and tropisetron (TRO) in goat plasma has been successfully developed and validated. This method procedure for the analysis of OND, GRA and TRO was involved of extracting samples with hydrophilic interaction liquid chromatography (HILIC) solid phase extraction (SPE) and determination by liquid chromatography coupled to tandem mass spectroscopy. An SPE method for the simultaneous extraction of OND, GRA and TRO with high efficiency and selectivity was developed. Prior to HPLC-MS/MS analysis, most of the sources of interference present in the supernatant after protein precipitation of plasma proteins was efficiently removed from the samples by the HILIC SPE treatment. For the quantification of OND, GRA and TRO in the samples, tandem mass spectrometry operating in positive electrospray ionization mode with multiple reaction monitoring was used. The calibration curve was performed in the range of 0.2-20 ng/mL for the target OND, GRA and TRO in goat plasma samples. The precision of the intra- and inter-day assay for OND, GRA and TRO were 1.84-6.23% and 3.89-5.31%, 2.63-6.29% and 3.76-5.31%, 1.99-5.67% and 2.64-4.70%, respectively. The accuracy of the intra- and inter-day assay for OND, GRA and TRO were 89.15-97.39% and 89.46-95.17%, 91.08-100.82% and 91.24-99.47%, 92.30-100.74% and 94.21-97.90%, respectively. For the determination of OND, GRA and TRO in plasma samples, no significant matrix effects were observed. The mean absolute recoveries were 103-150%, 115-121%, and 98-141% for OND, GRA and TRO, respectively. Furthermore, the mean process efficiency values of silica SPE were 98-135%, 92-124%, and 72-109% for OND, GRA and TRO, respectively. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/30053687/Quantification_of_ondansetron_granisetron_and_tropisetron_in_goat_plasma_using_hydrophilic_interaction_liquid_chromatography_solid_phase_extraction_coupled_with_hydrophilic_interaction_liquid_chromatography_triple_quadrupole_tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -
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