Tags

Type your tag names separated by a space and hit enter

Alternate life history phases of a common seaweed have distinct microbial surface communities.
Mol Ecol. 2018 09; 27(17):3555-3568.ME

Abstract

Macroalgal life histories are complex, often involving the alternation of distinct free-living life history phases that differ in morphology, longevity and ploidy. The surfaces of marine macroalgae support diverse microbial biofilms, yet the degree of microbial variation between alternate phases is unknown. We quantified bacterial (16S rRNA gene) and microeukaryote (18S rRNA gene) communities on the surface of the common intertidal seaweed, Mastocarpus spp., which alternates between gametophyte (foliose, haploid) and sporophyte (encrusting, diploid) life history phases. A large portion (97%) of bacterial taxa on the surface Mastocarpus was also present in samples from the environment, indicating that macroalgal surface communities are largely assembled from the surrounding seawater. Still, changes in the relative abundance of bacterial taxa result in significantly different communities on alternate Mastocarpus life history phases, rocky substrate and seawater at all intertidal elevations. For microeukaryote assemblages, only high intertidal samples had significant differences between life history phases although sporophytes were not different from the rocky substrate at this elevation; gametophytes and sporophytes did not differ in microeukaryote communities in the mid and low zones. By sequencing three host genes, we identified three cryptic species of Mastocarpus in our data set, which co-occur in the mid-to-low intertidal zone. In these samples, M. alaskensis sporophytes harboured distinct bacterial communities compared to M. agardhii and M. intermedius sporophytes, which were not distinguishable. Conversely, microeukaryote communities did not differ among species.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Lemay MA
Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada. Hakai Institute, Heriot Bay, British Columbia, Canada.
Martone PT
Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada. Hakai Institute, Heriot Bay, British Columbia, Canada.
Hind KR
Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada. Hakai Institute, Heriot Bay, British Columbia, Canada.
Lindstrom SC
Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada. Hakai Institute, Heriot Bay, British Columbia, Canada.
Wegener Parfrey L
Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada. Hakai Institute, Heriot Bay, British Columbia, Canada. Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada.

MeSH

BacteriaBritish ColumbiaMicrobiotaRNA, Ribosomal, 16SRNA, Ribosomal, 18SRhodophytaSeaweedSpecies Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30055017

Citation

Lemay, Matthew A., et al. "Alternate Life History Phases of a Common Seaweed Have Distinct Microbial Surface Communities." Molecular Ecology, vol. 27, no. 17, 2018, pp. 3555-3568.
Lemay MA, Martone PT, Hind KR, et al. Alternate life history phases of a common seaweed have distinct microbial surface communities. Mol Ecol. 2018;27(17):3555-3568.
Lemay, M. A., Martone, P. T., Hind, K. R., Lindstrom, S. C., & Wegener Parfrey, L. (2018). Alternate life history phases of a common seaweed have distinct microbial surface communities. Molecular Ecology, 27(17), 3555-3568. https://doi.org/10.1111/mec.14815
Lemay MA, et al. Alternate Life History Phases of a Common Seaweed Have Distinct Microbial Surface Communities. Mol Ecol. 2018;27(17):3555-3568. PubMed PMID: 30055017.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alternate life history phases of a common seaweed have distinct microbial surface communities. AU - Lemay,Matthew A, AU - Martone,Patrick T, AU - Hind,Katharine R, AU - Lindstrom,Sandra C, AU - Wegener Parfrey,Laura, Y1 - 2018/08/16/ PY - 2017/08/22/received PY - 2018/07/11/revised PY - 2018/07/19/accepted PY - 2018/7/29/pubmed PY - 2019/6/30/medline PY - 2018/7/29/entrez KW - Mastocarpus KW - bacteria KW - macroalgae KW - marine microbes KW - microbial ecology SP - 3555 EP - 3568 JF - Molecular ecology JO - Mol Ecol VL - 27 IS - 17 N2 - Macroalgal life histories are complex, often involving the alternation of distinct free-living life history phases that differ in morphology, longevity and ploidy. The surfaces of marine macroalgae support diverse microbial biofilms, yet the degree of microbial variation between alternate phases is unknown. We quantified bacterial (16S rRNA gene) and microeukaryote (18S rRNA gene) communities on the surface of the common intertidal seaweed, Mastocarpus spp., which alternates between gametophyte (foliose, haploid) and sporophyte (encrusting, diploid) life history phases. A large portion (97%) of bacterial taxa on the surface Mastocarpus was also present in samples from the environment, indicating that macroalgal surface communities are largely assembled from the surrounding seawater. Still, changes in the relative abundance of bacterial taxa result in significantly different communities on alternate Mastocarpus life history phases, rocky substrate and seawater at all intertidal elevations. For microeukaryote assemblages, only high intertidal samples had significant differences between life history phases although sporophytes were not different from the rocky substrate at this elevation; gametophytes and sporophytes did not differ in microeukaryote communities in the mid and low zones. By sequencing three host genes, we identified three cryptic species of Mastocarpus in our data set, which co-occur in the mid-to-low intertidal zone. In these samples, M. alaskensis sporophytes harboured distinct bacterial communities compared to M. agardhii and M. intermedius sporophytes, which were not distinguishable. Conversely, microeukaryote communities did not differ among species. SN - 1365-294X UR - https://www.unboundmedicine.com/medline/citation/30055017/Alternate_life_history_phases_of_a_common_seaweed_have_distinct_microbial_surface_communities_ DB - PRIME DP - Unbound Medicine ER -