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Molecular basis of quinolone resistance in clinical isolates of Klebsiella pneumoniae from Pakistan.
Pak J Pharm Sci. 2018 Jul; 31(4(Supplementary)):1591-1596.PJ

Abstract

Antibiotic resistant Klebsiella pneumoniae, is associated with various nosocomial infections that are difficult to treat. This study is designed to find out the patterns of resistance against commonly used antibiotics in K. pneumoniae clinical isolates with special attention to fluoroquinolones. A total number of 200 K. pneumoniae clinical isolates collected from various tertiary care hospitals of Punjab, Pakistan for a span of 1 year were investigated. Isolates were identified biochemically and genetically using VITEK® system and species-specific PCR, respectively. Antibiogram of isolates was studied by using disc diffusion and broth micro-dilution assays. Highest infection of K. pneumoniae detected in urinary tract (43%) followed by respiratory tract (25.5%). Most of the isolates displayed strong resistance against ampicillin, cefotetan, tazobactam, cefuroxime, cefixime, ceftriaxone, ampicillin-sulbactam imipenem, meropenem, ciprofloxacin and moxifloxacin, while sensetive to cefotaxime. Chromosoaml mutation was deteted in gyrA gene, gyrA harbors a strong mutation which provides resistance against ciprofloxacin by substituting Ser83→Ile. However, no mutation was detected in gyrB gene. Moreover, qnrB1 plasmid born resistant gene was only detected among qnrA, qnrB and qnrS. The story depicts an alarming situation of antibiotic resistance among K. pneumoniae associated with various nosocomial infections.

Authors+Show Affiliations

Department of Microbiology, Government College University, Faisalabad, Pakistan.Department of Microbiology, Government College University, Faisalabad, Pakistan.School of Biological Sciences, University of the Punjab, Lahore, Pakistan.Department of Microbiology, Government College University, Faisalabad, Pakistan.Department of Microbiology, Government College University, Faisalabad, Pakistan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30058553

Citation

Alvi, Roman Farooq, et al. "Molecular Basis of Quinolone Resistance in Clinical Isolates of Klebsiella Pneumoniae From Pakistan." Pakistan Journal of Pharmaceutical Sciences, vol. 31, no. 4(Supplementary), 2018, pp. 1591-1596.
Alvi RF, Aslam B, Shahzad N, et al. Molecular basis of quinolone resistance in clinical isolates of Klebsiella pneumoniae from Pakistan. Pak J Pharm Sci. 2018;31(4(Supplementary)):1591-1596.
Alvi, R. F., Aslam, B., Shahzad, N., Rasool, M. H., & Shafique, M. (2018). Molecular basis of quinolone resistance in clinical isolates of Klebsiella pneumoniae from Pakistan. Pakistan Journal of Pharmaceutical Sciences, 31(4(Supplementary)), 1591-1596.
Alvi RF, et al. Molecular Basis of Quinolone Resistance in Clinical Isolates of Klebsiella Pneumoniae From Pakistan. Pak J Pharm Sci. 2018;31(4(Supplementary)):1591-1596. PubMed PMID: 30058553.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular basis of quinolone resistance in clinical isolates of Klebsiella pneumoniae from Pakistan. AU - Alvi,Roman Farooq, AU - Aslam,Bilal, AU - Shahzad,Naveed, AU - Rasool,Muhammad Hidayat, AU - Shafique,Muhammad, PY - 2018/7/31/entrez PY - 2018/7/31/pubmed PY - 2019/8/10/medline SP - 1591 EP - 1596 JF - Pakistan journal of pharmaceutical sciences JO - Pak J Pharm Sci VL - 31 IS - 4(Supplementary) N2 - Antibiotic resistant Klebsiella pneumoniae, is associated with various nosocomial infections that are difficult to treat. This study is designed to find out the patterns of resistance against commonly used antibiotics in K. pneumoniae clinical isolates with special attention to fluoroquinolones. A total number of 200 K. pneumoniae clinical isolates collected from various tertiary care hospitals of Punjab, Pakistan for a span of 1 year were investigated. Isolates were identified biochemically and genetically using VITEK® system and species-specific PCR, respectively. Antibiogram of isolates was studied by using disc diffusion and broth micro-dilution assays. Highest infection of K. pneumoniae detected in urinary tract (43%) followed by respiratory tract (25.5%). Most of the isolates displayed strong resistance against ampicillin, cefotetan, tazobactam, cefuroxime, cefixime, ceftriaxone, ampicillin-sulbactam imipenem, meropenem, ciprofloxacin and moxifloxacin, while sensetive to cefotaxime. Chromosoaml mutation was deteted in gyrA gene, gyrA harbors a strong mutation which provides resistance against ciprofloxacin by substituting Ser83→Ile. However, no mutation was detected in gyrB gene. Moreover, qnrB1 plasmid born resistant gene was only detected among qnrA, qnrB and qnrS. The story depicts an alarming situation of antibiotic resistance among K. pneumoniae associated with various nosocomial infections. SN - 1011-601X UR - https://www.unboundmedicine.com/medline/citation/30058553/Molecular_basis_of_quinolone_resistance_in_clinical_isolates_of_Klebsiella_pneumoniae_from_Pakistan L2 - http://www.diseaseinfosearch.org/result/4006 DB - PRIME DP - Unbound Medicine ER -