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Semi-aquatic spider silks: transcripts, proteins, and silk fibres of the fishing spider, Dolomedes triton (Pisauridae).
Insect Mol Biol 2019; 28(1):35-51IM

Abstract

To survive in terrestrial and aquatic environments, spiders often rely heavily on their silk. The vast majority of silks that have been studied are from orb-web or cob-web weaving species, leaving the silks of water-associated spiders largely undescribed. We characterize transcripts, proteins, and silk fibres from the semi-aquatic spider Dolomedes triton. From silk gland RNAseq libraries, we report 18 silk transcripts representing four categories of known silk protein types: aciniform, ampullate, pyriform, and tubuliform. Proteomic and structural analyses (scanning electron microscopy, energy dispersive X-ray spectrometry, contact angle) of the D. triton submersible egg sac reveal similarities to silks from aquatic caddisfly larvae. We identified two layers in D. triton egg sacs, notably a highly hydrophobic outer layer with a different elemental composition compared to egg sacs of terrestrial spiders. These features may provide D. triton egg sacs with their water repellent properties.

Authors+Show Affiliations

Department of Evolution, Ecology, and Organismal Biology, University of California, Riverside, CA, USA.Department of Evolution, Ecology, and Organismal Biology, University of California, Riverside, CA, USA. Department of Neurology, Oregon Health and Science University, Portland, OR, USA.Materials Science and Engineering Program, University of California, Riverside, CA, USA.Department of Evolution, Ecology, and Organismal Biology, University of California, Riverside, CA, USA. J. Craig Venter Institute, Rockville, MD, USA.Materials Science and Engineering Program, University of California, Riverside, CA, USA.Materials Science and Engineering Program, University of California, Riverside, CA, USA. Department of Chemical and Environmental Engineering, University of California, Riverside, CA, USA.Department of Evolution, Ecology, and Organismal Biology, University of California, Riverside, CA, USA. Division of Invertebrate Zoology and Sackler Institute for Comparative Genomics, American Museum of Natural History, New York, NY, USA.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

30059178

Citation

Correa-Garhwal, S M., et al. "Semi-aquatic Spider Silks: Transcripts, Proteins, and Silk Fibres of the Fishing Spider, Dolomedes Triton (Pisauridae)." Insect Molecular Biology, vol. 28, no. 1, 2019, pp. 35-51.
Correa-Garhwal SM, Chaw RC, Dugger T, et al. Semi-aquatic spider silks: transcripts, proteins, and silk fibres of the fishing spider, Dolomedes triton (Pisauridae). Insect Mol Biol. 2019;28(1):35-51.
Correa-Garhwal, S. M., Chaw, R. C., Dugger, T., Clarke, T. H., Chea, K. H., Kisailus, D., & Hayashi, C. Y. (2019). Semi-aquatic spider silks: transcripts, proteins, and silk fibres of the fishing spider, Dolomedes triton (Pisauridae). Insect Molecular Biology, 28(1), pp. 35-51. doi:10.1111/imb.12527.
Correa-Garhwal SM, et al. Semi-aquatic Spider Silks: Transcripts, Proteins, and Silk Fibres of the Fishing Spider, Dolomedes Triton (Pisauridae). Insect Mol Biol. 2019;28(1):35-51. PubMed PMID: 30059178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Semi-aquatic spider silks: transcripts, proteins, and silk fibres of the fishing spider, Dolomedes triton (Pisauridae). AU - Correa-Garhwal,S M, AU - Chaw,R C, AU - Dugger,T, AU - Clarke,T H,3rd AU - Chea,K H, AU - Kisailus,D, AU - Hayashi,C Y, Y1 - 2018/10/16/ PY - 2018/7/31/pubmed PY - 2019/8/3/medline PY - 2018/7/31/entrez KW - Pisauridae KW - egg sacs KW - fishing spider KW - hydrophobicity KW - semi-aquatic spiders KW - spider silks KW - spidroins SP - 35 EP - 51 JF - Insect molecular biology JO - Insect Mol. Biol. VL - 28 IS - 1 N2 - To survive in terrestrial and aquatic environments, spiders often rely heavily on their silk. The vast majority of silks that have been studied are from orb-web or cob-web weaving species, leaving the silks of water-associated spiders largely undescribed. We characterize transcripts, proteins, and silk fibres from the semi-aquatic spider Dolomedes triton. From silk gland RNAseq libraries, we report 18 silk transcripts representing four categories of known silk protein types: aciniform, ampullate, pyriform, and tubuliform. Proteomic and structural analyses (scanning electron microscopy, energy dispersive X-ray spectrometry, contact angle) of the D. triton submersible egg sac reveal similarities to silks from aquatic caddisfly larvae. We identified two layers in D. triton egg sacs, notably a highly hydrophobic outer layer with a different elemental composition compared to egg sacs of terrestrial spiders. These features may provide D. triton egg sacs with their water repellent properties. SN - 1365-2583 UR - https://www.unboundmedicine.com/medline/citation/30059178/Semi_aquatic_spider_silks:_transcripts_proteins_and_silk_fibres_of_the_fishing_spider_Dolomedes_triton__Pisauridae__ L2 - https://doi.org/10.1111/imb.12527 DB - PRIME DP - Unbound Medicine ER -