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Improvement of cellulase production in Trichoderma reesei Rut-C30 by overexpression of a novel regulatory gene Trvib-1.
Bioresour Technol. 2018 Jan; 247:676-683.BT

Abstract

Trichoderma reesei is a widely used cellulase producer, and development of robust strains for improved cellulase production is of great interest. In this study, the gene Trvib-1 encoding a putative transcription factor was overexpressed in T. reesei Rut-C30, and effects on cellulase production by the manipulation as well as corn stover degradation by the crude enzyme were investigated. Cellulase production and protein secretion were significantly improved in the culture of the recombinant T. reesei Vib-1, which were 200% and 219%, respectively, higher than that produced by the parent strain. Cellulase induction was enhanced in the presence of pure cellulose as well as various soluble inducers. Glucose released from the pretreated corn stover hydrolyzed by the crude enzyme in the recombinant strain was improved 40%. These results indicate that the overexpression of Trvib-1 is a feasible strategy for producing cellulase to enhance bioconversion efficiency of lignocellulosic biomass.

Authors+Show Affiliations

State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: xqzhao@sjtu.edu.cn.State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30060399

Citation

Zhang, Fei, et al. "Improvement of Cellulase Production in Trichoderma Reesei Rut-C30 By Overexpression of a Novel Regulatory Gene Trvib-1." Bioresource Technology, vol. 247, 2018, pp. 676-683.
Zhang F, Zhao X, Bai F. Improvement of cellulase production in Trichoderma reesei Rut-C30 by overexpression of a novel regulatory gene Trvib-1. Bioresour Technol. 2018;247:676-683.
Zhang, F., Zhao, X., & Bai, F. (2018). Improvement of cellulase production in Trichoderma reesei Rut-C30 by overexpression of a novel regulatory gene Trvib-1. Bioresource Technology, 247, 676-683. https://doi.org/10.1016/j.biortech.2017.09.126
Zhang F, Zhao X, Bai F. Improvement of Cellulase Production in Trichoderma Reesei Rut-C30 By Overexpression of a Novel Regulatory Gene Trvib-1. Bioresour Technol. 2018;247:676-683. PubMed PMID: 30060399.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improvement of cellulase production in Trichoderma reesei Rut-C30 by overexpression of a novel regulatory gene Trvib-1. AU - Zhang,Fei, AU - Zhao,Xinqing, AU - Bai,Fengwu, Y1 - 2017/09/20/ PY - 2017/07/31/received PY - 2017/09/17/revised PY - 2017/09/18/accepted PY - 2018/8/1/entrez PY - 2018/8/1/pubmed PY - 2018/10/12/medline KW - Cellulase production KW - Hydrolysis of lignocellulosic biomass KW - Protein secretion KW - Trichoderma reesei KW - Trvib-1 SP - 676 EP - 683 JF - Bioresource technology JO - Bioresour. Technol. VL - 247 N2 - Trichoderma reesei is a widely used cellulase producer, and development of robust strains for improved cellulase production is of great interest. In this study, the gene Trvib-1 encoding a putative transcription factor was overexpressed in T. reesei Rut-C30, and effects on cellulase production by the manipulation as well as corn stover degradation by the crude enzyme were investigated. Cellulase production and protein secretion were significantly improved in the culture of the recombinant T. reesei Vib-1, which were 200% and 219%, respectively, higher than that produced by the parent strain. Cellulase induction was enhanced in the presence of pure cellulose as well as various soluble inducers. Glucose released from the pretreated corn stover hydrolyzed by the crude enzyme in the recombinant strain was improved 40%. These results indicate that the overexpression of Trvib-1 is a feasible strategy for producing cellulase to enhance bioconversion efficiency of lignocellulosic biomass. SN - 1873-2976 UR - https://www.unboundmedicine.com/medline/citation/30060399/Improvement_of_cellulase_production_in_Trichoderma_reesei_Rut_C30_by_overexpression_of_a_novel_regulatory_gene_Trvib_1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(17)31691-7 DB - PRIME DP - Unbound Medicine ER -