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Moxibustion treatment modulates the gut microbiota and immune function in a dextran sulphate sodium-induced colitis rat model.
World J Gastroenterol. 2018 Jul 28; 24(28):3130-3144.WJ

Abstract

AIM

To investigate the effect and mechanism of moxibustion in rats with ulcerative colitis.

METHODS

A rat colitis model was established by administering 4% dextran sulphate sodium solution. Seventy male rats were randomly divided into seven groups: Healthy controls (HC), ulcerative colitis model group (UC), UC with 7 d of moxibustion (UC-7), UC with 14 d of moxibustion (UC-14), UC with mesalazine gavage (UC-W), HC with 7 d of moxibustion (HC-7), HC with 14 d of moxibustion (HC-14). Moxibustion was applied to the bilateral Tianshu (ST25). Gut microbiome profiling was conducted by 16S rRNA amplicon sequencing, and PCR and ELISA determined the expression of inflammatory cytokines in colon mucosa and serum, respectively.

RESULTS

Moxibustion treatment restored the colonic mucosa and decreased submucosal inflammatory cell infiltration in colitis rats. Rats treated with moxibustion and mesalazine had significantly lower levels of the dominant phyla Proteobacteria and the genera Saccharibacteria, Sphingomonas and Barnesiella than colitis rats, and they could restore the microbiome to levels similar to those observed in healthy rats. UC rats had reduced alpha diversity, which could be alleviated by moxibustion therapy, and UC-7 had a higher alpha diversity than UC-14. This finding suggests that short-term (7 d) but no longer term (14 d) moxibustion treatment may significantly affect the gut microbiome. The potential bacterial functions affected by moxibustion may be ascorbate and aldarate metabolism, and amino acid metabolism. Compared with HC group, the levels of the cytokines interleukin-12 (IL-12) (P < 0.05) and IL-6, IL-17, IL-23, interferon-γ, lipopolysaccharide, IgA, tumour necrosis factor-α and its receptors 1 (TNFR1) and TNFR2 (P < 0.01) were all increased, whereas anti-inflammatory cytokine IL-2 and IL-10 (P < 0.01) and transforming growth factor-β (P < 0.05) were decreased in UC rats. These changes were reversed by moxibustion.

CONCLUSION

Our findings suggest that moxibustion exerts its therapeutic effect by repairing mucosal tissue damage and modulating the gut microbiome and intestinal mucosal immunity.

Authors+Show Affiliations

Yueyang Clinical Medical College, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.Yueyang Clinical Medical College, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.Stark Neurosciences Research Institute and Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN 46202, United States.Zhejiang Institute for Food and Drug Control, Hangzhou 310052, Zhejiang Province, China.Yueyang Clinical Medical College, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.Shanghai Research Institute of Acupuncture-moxibustion and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China.Shanghai Research Institute of Acupuncture-moxibustion and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China.Shanghai Research Institute of Acupuncture-moxibustion and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China.Shanghai Research Institute of Acupuncture-moxibustion and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30065559

Citation

Qi, Qin, et al. "Moxibustion Treatment Modulates the Gut Microbiota and Immune Function in a Dextran Sulphate Sodium-induced Colitis Rat Model." World Journal of Gastroenterology, vol. 24, no. 28, 2018, pp. 3130-3144.
Qi Q, Liu YN, Jin XM, et al. Moxibustion treatment modulates the gut microbiota and immune function in a dextran sulphate sodium-induced colitis rat model. World J Gastroenterol. 2018;24(28):3130-3144.
Qi, Q., Liu, Y. N., Jin, X. M., Zhang, L. S., Wang, C., Bao, C. H., Liu, H. R., Wu, H. G., & Wang, X. M. (2018). Moxibustion treatment modulates the gut microbiota and immune function in a dextran sulphate sodium-induced colitis rat model. World Journal of Gastroenterology, 24(28), 3130-3144. https://doi.org/10.3748/wjg.v24.i28.3130
Qi Q, et al. Moxibustion Treatment Modulates the Gut Microbiota and Immune Function in a Dextran Sulphate Sodium-induced Colitis Rat Model. World J Gastroenterol. 2018 Jul 28;24(28):3130-3144. PubMed PMID: 30065559.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Moxibustion treatment modulates the gut microbiota and immune function in a dextran sulphate sodium-induced colitis rat model. AU - Qi,Qin, AU - Liu,Ya-Nan, AU - Jin,Xiao-Ming, AU - Zhang,Lin-Shuang, AU - Wang,Cun, AU - Bao,Chun-Hui, AU - Liu,Hui-Rong, AU - Wu,Huan-Gan, AU - Wang,Xiao-Mei, PY - 2018/04/09/received PY - 2018/05/22/revised PY - 2018/06/25/accepted PY - 2018/8/2/entrez PY - 2018/8/2/pubmed PY - 2018/11/6/medline KW - 16S rRNA KW - Gut microbiome KW - Inflammatory cytokine KW - Moxibustion KW - Ulcerative colitis SP - 3130 EP - 3144 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 24 IS - 28 N2 - AIM: To investigate the effect and mechanism of moxibustion in rats with ulcerative colitis. METHODS: A rat colitis model was established by administering 4% dextran sulphate sodium solution. Seventy male rats were randomly divided into seven groups: Healthy controls (HC), ulcerative colitis model group (UC), UC with 7 d of moxibustion (UC-7), UC with 14 d of moxibustion (UC-14), UC with mesalazine gavage (UC-W), HC with 7 d of moxibustion (HC-7), HC with 14 d of moxibustion (HC-14). Moxibustion was applied to the bilateral Tianshu (ST25). Gut microbiome profiling was conducted by 16S rRNA amplicon sequencing, and PCR and ELISA determined the expression of inflammatory cytokines in colon mucosa and serum, respectively. RESULTS: Moxibustion treatment restored the colonic mucosa and decreased submucosal inflammatory cell infiltration in colitis rats. Rats treated with moxibustion and mesalazine had significantly lower levels of the dominant phyla Proteobacteria and the genera Saccharibacteria, Sphingomonas and Barnesiella than colitis rats, and they could restore the microbiome to levels similar to those observed in healthy rats. UC rats had reduced alpha diversity, which could be alleviated by moxibustion therapy, and UC-7 had a higher alpha diversity than UC-14. This finding suggests that short-term (7 d) but no longer term (14 d) moxibustion treatment may significantly affect the gut microbiome. The potential bacterial functions affected by moxibustion may be ascorbate and aldarate metabolism, and amino acid metabolism. Compared with HC group, the levels of the cytokines interleukin-12 (IL-12) (P < 0.05) and IL-6, IL-17, IL-23, interferon-γ, lipopolysaccharide, IgA, tumour necrosis factor-α and its receptors 1 (TNFR1) and TNFR2 (P < 0.01) were all increased, whereas anti-inflammatory cytokine IL-2 and IL-10 (P < 0.01) and transforming growth factor-β (P < 0.05) were decreased in UC rats. These changes were reversed by moxibustion. CONCLUSION: Our findings suggest that moxibustion exerts its therapeutic effect by repairing mucosal tissue damage and modulating the gut microbiome and intestinal mucosal immunity. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/30065559/Moxibustion_treatment_modulates_the_gut_microbiota_and_immune_function_in_a_dextran_sulphate_sodium_induced_colitis_rat_model_ L2 - http://www.wjgnet.com/1007-9327/full/v24/i28/3130.htm DB - PRIME DP - Unbound Medicine ER -