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The Agropyron cristatum karyotype, chromosome structure and cross-genome homoeology as revealed by fluorescence in situ hybridization with tandem repeats and wheat single-gene probes.
Theor Appl Genet. 2018 Oct; 131(10):2213-2227.TA

Abstract

KEY MESSAGE

Fluorescence in situ hybridization with probes for 45 cDNAs and five tandem repeats revealed homoeologous relationships of Agropyron cristatum with wheat. The results will contribute to alien gene introgression in wheat improvement. Crested wheatgrass (Agropyron cristatum L. Gaertn.) is a wild relative of wheat and a promising source of novel genes for wheat improvement. To date, identification of A. cristatum chromosomes has not been possible, and its molecular karyotype has not been available. Furthermore, homoeologous relationship between the genomes of A. cristatum and wheat has not been determined. To develop chromosome-specific landmarks, A. cristatum genomic DNA was sequenced, and new tandem repeats were discovered. Their distribution on mitotic chromosomes was studied by fluorescence in situ hybridization (FISH), which revealed specific patterns for five repeats in addition to 5S and 45S ribosomal DNA and rye subtelomeric repeats pSc119.2 and pSc200. FISH with one tandem repeat together with 45S rDNA enabled identification of all A. cristatum chromosomes. To analyze the structure and cross-species homoeology of A. cristatum chromosomes with wheat, probes for 45 mapped wheat cDNAs covering all seven chromosome groups were localized by FISH. Thirty-four cDNAs hybridized to homoeologous chromosomes of A. cristatum, nine hybridized to homoeologous and non-homoeologous chromosomes, and two hybridized to unique positions on non-homoeologous chromosomes. FISH using single-gene probes revealed that the wheat-A. cristatum collinearity was distorted, and important structural rearrangements were observed for chromosomes 2P, 4P, 5P, 6P and 7P. Chromosomal inversions were found for pericentric region of 4P and whole chromosome arm 6PL. Furthermore, reciprocal translocations between 2PS and 4PL were detected. These results provide new insights into the genome evolution within Triticeae and will facilitate the use of crested wheatgrass in alien gene introgression into wheat.

Authors+Show Affiliations

Institute of Experimental Botany, Center of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 78371, Olomouc, Czech Republic. Field Crops Research Institute, Agricultural Research Centre, 9 Gamma Street, Giza, Cairo, 12619, Egypt.Institute of Experimental Botany, Center of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 78371, Olomouc, Czech Republic.Wheat Genetics Resource Center, Kansas State University, 1712 Claflin Road, 4024 Throckmorton PSC, Manhattan, KS, 66506, USA.Institute of Experimental Botany, Center of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 78371, Olomouc, Czech Republic.Institute of Experimental Botany, Center of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 78371, Olomouc, Czech Republic.Wheat Genetics Resource Center, Kansas State University, 1712 Claflin Road, 4024 Throckmorton PSC, Manhattan, KS, 66506, USA.Institute of Experimental Botany, Center of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 78371, Olomouc, Czech Republic.Wheat Genetics Resource Center, Kansas State University, 1712 Claflin Road, 4024 Throckmorton PSC, Manhattan, KS, 66506, USA.Institute of Experimental Botany, Center of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 78371, Olomouc, Czech Republic. vrana@ueb.cas.cz.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30069594

Citation

Said, Mahmoud, et al. "The Agropyron Cristatum Karyotype, Chromosome Structure and Cross-genome Homoeology as Revealed By Fluorescence in Situ Hybridization With Tandem Repeats and Wheat Single-gene Probes." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 131, no. 10, 2018, pp. 2213-2227.
Said M, Hřibová E, Danilova TV, et al. The Agropyron cristatum karyotype, chromosome structure and cross-genome homoeology as revealed by fluorescence in situ hybridization with tandem repeats and wheat single-gene probes. Theor Appl Genet. 2018;131(10):2213-2227.
Said, M., Hřibová, E., Danilova, T. V., Karafiátová, M., Čížková, J., Friebe, B., Doležel, J., Gill, B. S., & Vrána, J. (2018). The Agropyron cristatum karyotype, chromosome structure and cross-genome homoeology as revealed by fluorescence in situ hybridization with tandem repeats and wheat single-gene probes. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 131(10), 2213-2227. https://doi.org/10.1007/s00122-018-3148-9
Said M, et al. The Agropyron Cristatum Karyotype, Chromosome Structure and Cross-genome Homoeology as Revealed By Fluorescence in Situ Hybridization With Tandem Repeats and Wheat Single-gene Probes. Theor Appl Genet. 2018;131(10):2213-2227. PubMed PMID: 30069594.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Agropyron cristatum karyotype, chromosome structure and cross-genome homoeology as revealed by fluorescence in situ hybridization with tandem repeats and wheat single-gene probes. AU - Said,Mahmoud, AU - Hřibová,Eva, AU - Danilova,Tatiana V, AU - Karafiátová,Miroslava, AU - Čížková,Jana, AU - Friebe,Bernd, AU - Doležel,Jaroslav, AU - Gill,Bikram S, AU - Vrána,Jan, Y1 - 2018/08/01/ PY - 2018/01/08/received PY - 2018/07/23/accepted PY - 2018/8/3/pubmed PY - 2018/9/21/medline PY - 2018/8/3/entrez SP - 2213 EP - 2227 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 131 IS - 10 N2 - KEY MESSAGE: Fluorescence in situ hybridization with probes for 45 cDNAs and five tandem repeats revealed homoeologous relationships of Agropyron cristatum with wheat. The results will contribute to alien gene introgression in wheat improvement. Crested wheatgrass (Agropyron cristatum L. Gaertn.) is a wild relative of wheat and a promising source of novel genes for wheat improvement. To date, identification of A. cristatum chromosomes has not been possible, and its molecular karyotype has not been available. Furthermore, homoeologous relationship between the genomes of A. cristatum and wheat has not been determined. To develop chromosome-specific landmarks, A. cristatum genomic DNA was sequenced, and new tandem repeats were discovered. Their distribution on mitotic chromosomes was studied by fluorescence in situ hybridization (FISH), which revealed specific patterns for five repeats in addition to 5S and 45S ribosomal DNA and rye subtelomeric repeats pSc119.2 and pSc200. FISH with one tandem repeat together with 45S rDNA enabled identification of all A. cristatum chromosomes. To analyze the structure and cross-species homoeology of A. cristatum chromosomes with wheat, probes for 45 mapped wheat cDNAs covering all seven chromosome groups were localized by FISH. Thirty-four cDNAs hybridized to homoeologous chromosomes of A. cristatum, nine hybridized to homoeologous and non-homoeologous chromosomes, and two hybridized to unique positions on non-homoeologous chromosomes. FISH using single-gene probes revealed that the wheat-A. cristatum collinearity was distorted, and important structural rearrangements were observed for chromosomes 2P, 4P, 5P, 6P and 7P. Chromosomal inversions were found for pericentric region of 4P and whole chromosome arm 6PL. Furthermore, reciprocal translocations between 2PS and 4PL were detected. These results provide new insights into the genome evolution within Triticeae and will facilitate the use of crested wheatgrass in alien gene introgression into wheat. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/30069594/The_Agropyron_cristatum_karyotype_chromosome_structure_and_cross_genome_homoeology_as_revealed_by_fluorescence_in_situ_hybridization_with_tandem_repeats_and_wheat_single_gene_probes_ L2 - https://dx.doi.org/10.1007/s00122-018-3148-9 DB - PRIME DP - Unbound Medicine ER -