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Differences in the intensity of infection caused by Encephalitozoon cuniculi genotype II and III - Comparison using quantitative real-time PCR.
Exp Parasitol. 2018 Sep; 192:93-97.EP

Abstract

Microsporidia are a group of obligate intracellular eukaryotic parasites, which are able to infect a wide range of animals, including humans. Four genotypes of Encephalitozoon cuniculi have been found to date. The different courses of microsporidiosis described in humans, which are dependent on immunological status of the host and genotype of E. cuniculi, have been successfully imitated in murine models. In the present study, we quantified the microsporidial burden in individual organs of a murine experimental model, using qPCR and we compared the parasitic load of two genotypes of E. cuniculi, namely genotype II and III (EC II and EC III). While the extent of microsporidiosis caused by EC II gradually increased over 35 days post infection (DPI) in both immunocompetent and immunodeficient mice and caused death in the latter at 28 DPI, EC III had spread into all host organs by seven DPI and was not lethal for either mouse strain during the experimental time period. Moreover, EC III persisted in many organs until termination of the experiment. The number of microsporidial spores in individual organs was ten times higher in EC III-infected animals compared to those infected with EC II. EC II infection also progressively shifted towards organs outside the gastrointestinal tract (GIT) in both monitored mouse strains; whereas, EC III infection equally remained in both the GIT and organs outside the GIT. With the increasing use of molecular methods in diagnostics, it is important to better understand the pathophysiology of microsporidia, including its ability to escape from the immune system and persist in host organisms. Our results indicate that pathogenicity is not directly connected to spore burden, as infection caused by E. cuniculi genotype II is less extensive and spreads more slowly within the host organism than infection caused by E. cuniculi genotype III, but which caused the earlier death of immunodeficient mice.

Authors+Show Affiliations

Institute of Parasitology, Biology Centre, Czech Academy of Science, České Budějovice, Czech Republic; Faculty of Agriculture, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic.Institute of Parasitology, Biology Centre, Czech Academy of Science, České Budějovice, Czech Republic. Electronic address: casio@paru.cas.cz.Institute of Parasitology, Biology Centre, Czech Academy of Science, České Budějovice, Czech Republic; Faculty of Agriculture, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

30075234

Citation

Kotková, Michaela, et al. "Differences in the Intensity of Infection Caused By Encephalitozoon Cuniculi Genotype II and III - Comparison Using Quantitative Real-time PCR." Experimental Parasitology, vol. 192, 2018, pp. 93-97.
Kotková M, Sak B, Kváč M. Differences in the intensity of infection caused by Encephalitozoon cuniculi genotype II and III - Comparison using quantitative real-time PCR. Exp Parasitol. 2018;192:93-97.
Kotková, M., Sak, B., & Kváč, M. (2018). Differences in the intensity of infection caused by Encephalitozoon cuniculi genotype II and III - Comparison using quantitative real-time PCR. Experimental Parasitology, 192, 93-97. https://doi.org/10.1016/j.exppara.2018.07.019
Kotková M, Sak B, Kváč M. Differences in the Intensity of Infection Caused By Encephalitozoon Cuniculi Genotype II and III - Comparison Using Quantitative Real-time PCR. Exp Parasitol. 2018;192:93-97. PubMed PMID: 30075234.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differences in the intensity of infection caused by Encephalitozoon cuniculi genotype II and III - Comparison using quantitative real-time PCR. AU - Kotková,Michaela, AU - Sak,Bohumil, AU - Kváč,Martin, Y1 - 2018/07/31/ PY - 2018/04/19/received PY - 2018/07/20/revised PY - 2018/07/30/accepted PY - 2018/8/4/pubmed PY - 2018/9/12/medline PY - 2018/8/4/entrez KW - BALB/C KW - Encephalitozoon cuniculi genotypes II and III KW - Microsporidiosis KW - SCID KW - qRT-PCR SP - 93 EP - 97 JF - Experimental parasitology JO - Exp. Parasitol. VL - 192 N2 - Microsporidia are a group of obligate intracellular eukaryotic parasites, which are able to infect a wide range of animals, including humans. Four genotypes of Encephalitozoon cuniculi have been found to date. The different courses of microsporidiosis described in humans, which are dependent on immunological status of the host and genotype of E. cuniculi, have been successfully imitated in murine models. In the present study, we quantified the microsporidial burden in individual organs of a murine experimental model, using qPCR and we compared the parasitic load of two genotypes of E. cuniculi, namely genotype II and III (EC II and EC III). While the extent of microsporidiosis caused by EC II gradually increased over 35 days post infection (DPI) in both immunocompetent and immunodeficient mice and caused death in the latter at 28 DPI, EC III had spread into all host organs by seven DPI and was not lethal for either mouse strain during the experimental time period. Moreover, EC III persisted in many organs until termination of the experiment. The number of microsporidial spores in individual organs was ten times higher in EC III-infected animals compared to those infected with EC II. EC II infection also progressively shifted towards organs outside the gastrointestinal tract (GIT) in both monitored mouse strains; whereas, EC III infection equally remained in both the GIT and organs outside the GIT. With the increasing use of molecular methods in diagnostics, it is important to better understand the pathophysiology of microsporidia, including its ability to escape from the immune system and persist in host organisms. Our results indicate that pathogenicity is not directly connected to spore burden, as infection caused by E. cuniculi genotype II is less extensive and spreads more slowly within the host organism than infection caused by E. cuniculi genotype III, but which caused the earlier death of immunodeficient mice. SN - 1090-2449 UR - https://www.unboundmedicine.com/medline/citation/30075234/Differences_in_the_intensity_of_infection_caused_by_Encephalitozoon_cuniculi_genotype_II_and_III___Comparison_using_quantitative_real_time_PCR_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4894(18)30216-9 DB - PRIME DP - Unbound Medicine ER -