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Evaluation of the immune responses following co-administration of PilQ and type b-flagellin from Pseudomonas aeruginosa in the burn mouse model.
Microb Pathog. 2018 Oct; 123:426-432.MP

Abstract

Considering the increased antibiotic resistance of Pseudomonas aeruginosa, the evaluation of immune response against the antigens of this bacterium seems necessary. In this study, the protective efficacy and immunological properties of P. aeruginosa recombinant PilQ (r-PilQ) and type b-flagellin (FLB) proteins was evaluated in the burn mouse model of infection. The inbred BALB/c mice were immunized with r-PilQ and FLB antigens. To investigate the type of induced immune response, sera were analyzed by ELISA for total IgG, IgG1, and IgG2a isotypes. After the final immunization, the IL-4, IFN-γ, and IL-17 cytokines level were examined in the spleen of non-challenged mice. Fifty days after lethal challenge, the survival rate and bacterial burden in the skin and other internal organs of experimental mice were assessed. The in vivo administration of r-PilQ, FLB and combined antigen resulted in a significant increase in the survival of mice (66%, 75%, and 83%, respectively) infected by the PAO1 strain of P. aeruginosa in the burn model of infection. Immunization of mice with r-PilQ and FLB mixture induced high titers of IL-4 and IL-17 cytokines compared to control groups (P < 0.05). The high titer of antisera raised against combined antigen was able to inhibit the systemic spread of the PAO1 strain from the site of infection to the internal organs. We concluded that the parallel role of IL-4 and IL-17 is necessary for elimination of the bacteria and promotion of survival in the immunized burn mice.

Authors+Show Affiliations

Medical Biotechnology Research Center, Laboratory of Microbiology and Immunology of Infectious Diseases, Paramedicine Faculty, Guilan University of Medical Sciences, Rasht, Iran.Medical Biotechnology Research Center, Laboratory of Microbiology and Immunology of Infectious Diseases, Paramedicine Faculty, Guilan University of Medical Sciences, Rasht, Iran; Medical Biotechnology Research Center, Paramedicine Faculty, Guilan University of Medical Sciences, Rasht, Iran. Electronic address: Nikokariraj@yahoo.com.Medical Biotechnology Research Center, Paramedicine Faculty, Guilan University of Medical Sciences, Rasht, Iran.Medical Biotechnology Research Center, Laboratory of Microbiology and Immunology of Infectious Diseases, Paramedicine Faculty, Guilan University of Medical Sciences, Rasht, Iran.Recombinant Vaccine Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Immunology, Pasteur Institute of Iran, Tehran, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30075242

Citation

Bakht Azad, Sima, et al. "Evaluation of the Immune Responses Following Co-administration of PilQ and Type B-flagellin From Pseudomonas Aeruginosa in the Burn Mouse Model." Microbial Pathogenesis, vol. 123, 2018, pp. 426-432.
Bakht Azad S, Nikokar I, Faezi S, et al. Evaluation of the immune responses following co-administration of PilQ and type b-flagellin from Pseudomonas aeruginosa in the burn mouse model. Microb Pathog. 2018;123:426-432.
Bakht Azad, S., Nikokar, I., Faezi, S., Rasooly, S., & Mahdavi, M. (2018). Evaluation of the immune responses following co-administration of PilQ and type b-flagellin from Pseudomonas aeruginosa in the burn mouse model. Microbial Pathogenesis, 123, 426-432. https://doi.org/10.1016/j.micpath.2018.07.042
Bakht Azad S, et al. Evaluation of the Immune Responses Following Co-administration of PilQ and Type B-flagellin From Pseudomonas Aeruginosa in the Burn Mouse Model. Microb Pathog. 2018;123:426-432. PubMed PMID: 30075242.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the immune responses following co-administration of PilQ and type b-flagellin from Pseudomonas aeruginosa in the burn mouse model. AU - Bakht Azad,Sima, AU - Nikokar,Iraj, AU - Faezi,Sobhan, AU - Rasooly,Soheila, AU - Mahdavi,Mehdi, Y1 - 2018/07/31/ PY - 2017/12/30/received PY - 2018/07/16/revised PY - 2018/07/30/accepted PY - 2018/8/4/pubmed PY - 2018/12/29/medline PY - 2018/8/4/entrez KW - Burn KW - Cytokine KW - Flagellin KW - Immunization KW - PilQ KW - Pseudomonas aeruginosa SP - 426 EP - 432 JF - Microbial pathogenesis JO - Microb. Pathog. VL - 123 N2 - Considering the increased antibiotic resistance of Pseudomonas aeruginosa, the evaluation of immune response against the antigens of this bacterium seems necessary. In this study, the protective efficacy and immunological properties of P. aeruginosa recombinant PilQ (r-PilQ) and type b-flagellin (FLB) proteins was evaluated in the burn mouse model of infection. The inbred BALB/c mice were immunized with r-PilQ and FLB antigens. To investigate the type of induced immune response, sera were analyzed by ELISA for total IgG, IgG1, and IgG2a isotypes. After the final immunization, the IL-4, IFN-γ, and IL-17 cytokines level were examined in the spleen of non-challenged mice. Fifty days after lethal challenge, the survival rate and bacterial burden in the skin and other internal organs of experimental mice were assessed. The in vivo administration of r-PilQ, FLB and combined antigen resulted in a significant increase in the survival of mice (66%, 75%, and 83%, respectively) infected by the PAO1 strain of P. aeruginosa in the burn model of infection. Immunization of mice with r-PilQ and FLB mixture induced high titers of IL-4 and IL-17 cytokines compared to control groups (P < 0.05). The high titer of antisera raised against combined antigen was able to inhibit the systemic spread of the PAO1 strain from the site of infection to the internal organs. We concluded that the parallel role of IL-4 and IL-17 is necessary for elimination of the bacteria and promotion of survival in the immunized burn mice. SN - 1096-1208 UR - https://www.unboundmedicine.com/medline/citation/30075242/Evaluation_of_the_immune_responses_following_co_administration_of_PilQ_and_type_b_flagellin_from_Pseudomonas_aeruginosa_in_the_burn_mouse_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0882-4010(17)31796-5 DB - PRIME DP - Unbound Medicine ER -