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Short-Term Exposure to Nitrogen Dioxide Provides Basal Pathogen Resistance.
Plant Physiol. 2018 09; 178(1):468-487.PP

Abstract

Nitrogen dioxide (NO2) forms in plants under stress conditions, but little is known about its physiological functions. Here, we explored the physiological functions of NO2 in plant cells using short-term fumigation of Arabidopsis (Arabidopsis thaliana) for 1 h with 10 µL L-1 NO2. Although leaf symptoms were absent, the expression of genes related to pathogen resistance was induced. Fumigated plants developed basal disease resistance, or pattern-triggered immunity, against the necrotrophic fungus Botrytis cinerea and the hemibiotrophic bacterium Pseudomonas syringae Functional salicylic acid and jasmonic acid (JA) signaling pathways were both required for the full expression of NO2-induced resistance against B. cinerea An early peak of salicylic acid accumulation immediately after NO2 exposure was followed by a transient accumulation of oxophytodienoic acid. The simultaneous NO2-induced expression of genes involved in jasmonate biosynthesis and jasmonate catabolism resulted in the complete suppression of JA and JA-isoleucine (JA-Ile) accumulation, which was accompanied by a rise in the levels of their catabolic intermediates 12-OH-JA, 12-OH-JA-Ile, and 12-COOH-JA-Ile. NO2-treated plants emitted the volatile monoterpene α-pinene and the sesquiterpene longifolene (syn. junipene), which could function in signaling or direct defense against pathogens. NO2-triggered B. cinerea resistance was dependent on enhanced early callose deposition and CYTOCHROME P450 79B2 (CYP79B2), CYP79B3, and PHYTOALEXIN DEFICIENT3 gene functions but independent of camalexin, CYP81F2, and 4-OH-indol-3-ylmethylglucosinolate derivatives. In sum, exogenous NO2 triggers basal pathogen resistance, pointing to a possible role for endogenous NO2 in defense signaling. Additionally, this study revealed the involvement of jasmonate catabolism and volatiles in pathogen immunity.

Authors+Show Affiliations

Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, German Research Center for Environmental Health, D-85764 Neuherberg, Germany.Max Planck Institute for Chemical Ecology, Department Bioorganic Chemistry, D-07745 Jena, Germany.Department of Plant Sciences, Technical University of Munich, D-85354 Freising, Germany.Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, German Research Center for Environmental Health, D-85764 Neuherberg, Germany.Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, German Research Center for Environmental Health, D-85764 Neuherberg, Germany.Analytical BioGeoChemistry, Helmholtz Zentrum München, German Research Center for Environmental Health, D-85764 Neuherberg, Germany.Analytical BioGeoChemistry, Helmholtz Zentrum München, German Research Center for Environmental Health, D-85764 Neuherberg, Germany.Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, German Research Center for Environmental Health, D-85764 Neuherberg, Germany.Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, German Research Center for Environmental Health, D-85764 Neuherberg, Germany.Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, German Research Center for Environmental Health, D-85764 Neuherberg, Germany frank.gaupels@helmholtz-muenchen.de.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30076223

Citation

Mayer, Dörte, et al. "Short-Term Exposure to Nitrogen Dioxide Provides Basal Pathogen Resistance." Plant Physiology, vol. 178, no. 1, 2018, pp. 468-487.
Mayer D, Mithöfer A, Glawischnig E, et al. Short-Term Exposure to Nitrogen Dioxide Provides Basal Pathogen Resistance. Plant Physiol. 2018;178(1):468-487.
Mayer, D., Mithöfer, A., Glawischnig, E., Georgii, E., Ghirardo, A., Kanawati, B., Schmitt-Kopplin, P., Schnitzler, J. P., Durner, J., & Gaupels, F. (2018). Short-Term Exposure to Nitrogen Dioxide Provides Basal Pathogen Resistance. Plant Physiology, 178(1), 468-487. https://doi.org/10.1104/pp.18.00704
Mayer D, et al. Short-Term Exposure to Nitrogen Dioxide Provides Basal Pathogen Resistance. Plant Physiol. 2018;178(1):468-487. PubMed PMID: 30076223.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Short-Term Exposure to Nitrogen Dioxide Provides Basal Pathogen Resistance. AU - Mayer,Dörte, AU - Mithöfer,Axel, AU - Glawischnig,Erich, AU - Georgii,Elisabeth, AU - Ghirardo,Andrea, AU - Kanawati,Basem, AU - Schmitt-Kopplin,Philippe, AU - Schnitzler,Jörg-Peter, AU - Durner,Jörg, AU - Gaupels,Frank, Y1 - 2018/08/03/ PY - 2018/06/08/received PY - 2018/07/27/accepted PY - 2018/8/5/pubmed PY - 2019/3/29/medline PY - 2018/8/5/entrez SP - 468 EP - 487 JF - Plant physiology JO - Plant Physiol VL - 178 IS - 1 N2 - Nitrogen dioxide (NO2) forms in plants under stress conditions, but little is known about its physiological functions. Here, we explored the physiological functions of NO2 in plant cells using short-term fumigation of Arabidopsis (Arabidopsis thaliana) for 1 h with 10 µL L-1 NO2. Although leaf symptoms were absent, the expression of genes related to pathogen resistance was induced. Fumigated plants developed basal disease resistance, or pattern-triggered immunity, against the necrotrophic fungus Botrytis cinerea and the hemibiotrophic bacterium Pseudomonas syringae Functional salicylic acid and jasmonic acid (JA) signaling pathways were both required for the full expression of NO2-induced resistance against B. cinerea An early peak of salicylic acid accumulation immediately after NO2 exposure was followed by a transient accumulation of oxophytodienoic acid. The simultaneous NO2-induced expression of genes involved in jasmonate biosynthesis and jasmonate catabolism resulted in the complete suppression of JA and JA-isoleucine (JA-Ile) accumulation, which was accompanied by a rise in the levels of their catabolic intermediates 12-OH-JA, 12-OH-JA-Ile, and 12-COOH-JA-Ile. NO2-treated plants emitted the volatile monoterpene α-pinene and the sesquiterpene longifolene (syn. junipene), which could function in signaling or direct defense against pathogens. NO2-triggered B. cinerea resistance was dependent on enhanced early callose deposition and CYTOCHROME P450 79B2 (CYP79B2), CYP79B3, and PHYTOALEXIN DEFICIENT3 gene functions but independent of camalexin, CYP81F2, and 4-OH-indol-3-ylmethylglucosinolate derivatives. In sum, exogenous NO2 triggers basal pathogen resistance, pointing to a possible role for endogenous NO2 in defense signaling. Additionally, this study revealed the involvement of jasmonate catabolism and volatiles in pathogen immunity. SN - 1532-2548 UR - https://www.unboundmedicine.com/medline/citation/30076223/Short_Term_Exposure_to_Nitrogen_Dioxide_Provides_Basal_Pathogen_Resistance_ L2 - http://www.plantphysiol.org/lookup/pmidlookup?view=long&pmid=30076223 DB - PRIME DP - Unbound Medicine ER -