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Mechanism mediating the protective effect of diacerein in ischemia-reperfusion-induced testicular injury in rats.
Life Sci. 2018 Sep 15; 209:57-62.LS

Abstract

AIMS

Torsion of the spermatic cord is a common urologic emergency and can lead to testicular necrosis and infertility. Diacerein (DIA) is interleukin-1b (IL-1b) blocker which has a protective role against myocardial ischemia-reperfusion, however, to-date this role has not been investigated in testicular ischemia- reperfusion (TIR). We aimed to investigate the role and mechanism of action of DIA in induced TIR.

MAIN METHODS

DIA (50 mg/kg) was administered i.m (intramuscular) to rats in the presence or absence of TIR. Testicular weight changes and serum testosterone and total cholesterol levels were evaluated. In addition; the level of testicular tissue reduced glutathione (GSH), malondialdehyde (MDA), total nitrites (NOx) and the activity of superoxide dismutase (SOD) were measured. Histopathology and interleukin1b (IL-1b) immunoexpression were evaluated.

KEY FINDINGS

TIR manifested by significant decrease in testicular weight, serum testosterone and testicular tissue GSH levels and SOD activity as well as increase in serum total cholesterol, testicular MDA and NOx levels. TIR showed the histopathological changes of marked testicular damage with increase in IL-1b immunoexpression. DIA was able to normalize both testicular weight, serum testosterone and cholesterol levels with attenuation of oxidative stress parameters along with amelioration of histopathological changes and IL-1 b immunostaining induced by TIR.

SIGNIFICANCE

DIA has a protective effect against TIR induced injury in rats mediated by its anti-inflammatory and anti-oxidant activities.

Authors+Show Affiliations

Department of Pharmacology, Faculty of Medicine, Minia University, 61511 Minia, Egypt. Electronic address: sehamabdelwakeel@yahoo.com.Department of Pathology, Faculty of Medicine, Minia University, 61511 Minia, Egypt.Department of Pharmacology, Faculty of Medicine, Minia University, 61511 Minia, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30076921

Citation

Abdel-Gaber, Seham A., et al. "Mechanism Mediating the Protective Effect of Diacerein in Ischemia-reperfusion-induced Testicular Injury in Rats." Life Sciences, vol. 209, 2018, pp. 57-62.
Abdel-Gaber SA, Mohammed RK, Refaie MMM. Mechanism mediating the protective effect of diacerein in ischemia-reperfusion-induced testicular injury in rats. Life Sci. 2018;209:57-62.
Abdel-Gaber, S. A., Mohammed, R. K., & Refaie, M. M. M. (2018). Mechanism mediating the protective effect of diacerein in ischemia-reperfusion-induced testicular injury in rats. Life Sciences, 209, 57-62. https://doi.org/10.1016/j.lfs.2018.07.060
Abdel-Gaber SA, Mohammed RK, Refaie MMM. Mechanism Mediating the Protective Effect of Diacerein in Ischemia-reperfusion-induced Testicular Injury in Rats. Life Sci. 2018 Sep 15;209:57-62. PubMed PMID: 30076921.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanism mediating the protective effect of diacerein in ischemia-reperfusion-induced testicular injury in rats. AU - Abdel-Gaber,Seham A, AU - Mohammed,Rehab K, AU - Refaie,Marwa M M, Y1 - 2018/08/01/ PY - 2018/07/17/received PY - 2018/07/28/revised PY - 2018/07/31/accepted PY - 2018/8/5/pubmed PY - 2018/10/4/medline PY - 2018/8/5/entrez KW - Diacerein KW - Histopathology KW - Immunoexpression KW - Inflammation KW - Oxidative stress KW - Testicular ischemia-reperfusion SP - 57 EP - 62 JF - Life sciences JO - Life Sci VL - 209 N2 - AIMS: Torsion of the spermatic cord is a common urologic emergency and can lead to testicular necrosis and infertility. Diacerein (DIA) is interleukin-1b (IL-1b) blocker which has a protective role against myocardial ischemia-reperfusion, however, to-date this role has not been investigated in testicular ischemia- reperfusion (TIR). We aimed to investigate the role and mechanism of action of DIA in induced TIR. MAIN METHODS: DIA (50 mg/kg) was administered i.m (intramuscular) to rats in the presence or absence of TIR. Testicular weight changes and serum testosterone and total cholesterol levels were evaluated. In addition; the level of testicular tissue reduced glutathione (GSH), malondialdehyde (MDA), total nitrites (NOx) and the activity of superoxide dismutase (SOD) were measured. Histopathology and interleukin1b (IL-1b) immunoexpression were evaluated. KEY FINDINGS: TIR manifested by significant decrease in testicular weight, serum testosterone and testicular tissue GSH levels and SOD activity as well as increase in serum total cholesterol, testicular MDA and NOx levels. TIR showed the histopathological changes of marked testicular damage with increase in IL-1b immunoexpression. DIA was able to normalize both testicular weight, serum testosterone and cholesterol levels with attenuation of oxidative stress parameters along with amelioration of histopathological changes and IL-1 b immunostaining induced by TIR. SIGNIFICANCE: DIA has a protective effect against TIR induced injury in rats mediated by its anti-inflammatory and anti-oxidant activities. SN - 1879-0631 UR - https://www.unboundmedicine.com/medline/citation/30076921/Mechanism_mediating_the_protective_effect_of_diacerein_in_ischemia_reperfusion_induced_testicular_injury_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(18)30446-6 DB - PRIME DP - Unbound Medicine ER -