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Global regulatory function of the low oxygen-induced transcriptional regulator LoiA in Salmonella Typhimurium revealed by RNA sequencing.
Biochem Biophys Res Commun. 2018 09 10; 503(3):2022-2027.BB

Abstract

Salmonella enterica serovar Typhimurium (S. Typhimurium) is a major intestinal pathogen that can infect both humans and a variety of animals. LoiA, a novel virulence-regulating protein encoded in Salmonella pathogenicity island (SPI)-14, has been shown to be induced under low oxygen conditions and contribute to S. Typhimurium invasion into intestinal epithetical cells by activating the SPI-1 invasion genes. However, the global regulatory network of LoiA remains unknown. Here, we used high-throughput RNA sequencing (RNA-seq) technology to investigate the regulatory function of LoiA in S. Typhimurium under low oxygen conditions. A total of 1250 genes were differentially expressed between the loiA mutant and the wild-type strain; 413 genes were up-regulated and 837 were down-regulated. SPI-1 gene expression was down-regulated in the loiA mutant, consistent with previous results. SPI-2 gene expression was not affected by deletion of loiA; the expression of most genes involved in flagellar basal body and hook biosynthesis was up-regulated in the loiA mutant, while the expression of genes associated with flagellin, motility, and chemotaxis was down-regulated; the expression of lon, encoding an ATP-dependent protease, was up-regulated in the mutant. This study indicates that LoiA regulates a variety of virulence-associated genes in S. Typhimurium. The negative regulation of Lon protease by LoiA indicates that LoiA can regulates several virulence-associated genes in S. Typhimurium via the Lon protease.

Authors+Show Affiliations

TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, China; Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, 300457, China; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin, 300457, China; College of Life Sciences, Nankai University, Tianjin, 300071, China.TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, China; Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, 300457, China; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin, 300457, China.TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, China; Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, 300457, China; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin, 300457, China.TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, China; Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, 300457, China; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin, 300457, China.TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, China; Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, 300457, China; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin, 300457, China; College of Life Sciences, Nankai University, Tianjin, 300071, China.TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, China; Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, 300457, China; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin, 300457, China; College of Life Sciences, Nankai University, Tianjin, 300071, China.TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, China; Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, 300457, China; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin, 300457, China. Electronic address: jianglingyan@nankai.edu.cn.TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, China; Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, 300457, China; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin, 300457, China. Electronic address: liubin1981@nankai.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30077369

Citation

Li, Huiying, et al. "Global Regulatory Function of the Low Oxygen-induced Transcriptional Regulator LoiA in Salmonella Typhimurium Revealed By RNA Sequencing." Biochemical and Biophysical Research Communications, vol. 503, no. 3, 2018, pp. 2022-2027.
Li H, Li X, Lv R, et al. Global regulatory function of the low oxygen-induced transcriptional regulator LoiA in Salmonella Typhimurium revealed by RNA sequencing. Biochem Biophys Res Commun. 2018;503(3):2022-2027.
Li, H., Li, X., Lv, R., Jiang, X., Cao, H., Du, Y., Jiang, L., & Liu, B. (2018). Global regulatory function of the low oxygen-induced transcriptional regulator LoiA in Salmonella Typhimurium revealed by RNA sequencing. Biochemical and Biophysical Research Communications, 503(3), 2022-2027. https://doi.org/10.1016/j.bbrc.2018.07.151
Li H, et al. Global Regulatory Function of the Low Oxygen-induced Transcriptional Regulator LoiA in Salmonella Typhimurium Revealed By RNA Sequencing. Biochem Biophys Res Commun. 2018 09 10;503(3):2022-2027. PubMed PMID: 30077369.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Global regulatory function of the low oxygen-induced transcriptional regulator LoiA in Salmonella Typhimurium revealed by RNA sequencing. AU - Li,Huiying, AU - Li,Xiaomin, AU - Lv,Runxia, AU - Jiang,Xiaolong, AU - Cao,Hengchun, AU - Du,Yuhui, AU - Jiang,Lingyan, AU - Liu,Bin, Y1 - 2018/08/01/ PY - 2018/07/23/received PY - 2018/07/30/accepted PY - 2018/8/6/pubmed PY - 2019/5/14/medline PY - 2018/8/6/entrez KW - LoiA KW - RNA sequencing KW - Regulatory network KW - Salmonella Typhimurium SP - 2022 EP - 2027 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 503 IS - 3 N2 - Salmonella enterica serovar Typhimurium (S. Typhimurium) is a major intestinal pathogen that can infect both humans and a variety of animals. LoiA, a novel virulence-regulating protein encoded in Salmonella pathogenicity island (SPI)-14, has been shown to be induced under low oxygen conditions and contribute to S. Typhimurium invasion into intestinal epithetical cells by activating the SPI-1 invasion genes. However, the global regulatory network of LoiA remains unknown. Here, we used high-throughput RNA sequencing (RNA-seq) technology to investigate the regulatory function of LoiA in S. Typhimurium under low oxygen conditions. A total of 1250 genes were differentially expressed between the loiA mutant and the wild-type strain; 413 genes were up-regulated and 837 were down-regulated. SPI-1 gene expression was down-regulated in the loiA mutant, consistent with previous results. SPI-2 gene expression was not affected by deletion of loiA; the expression of most genes involved in flagellar basal body and hook biosynthesis was up-regulated in the loiA mutant, while the expression of genes associated with flagellin, motility, and chemotaxis was down-regulated; the expression of lon, encoding an ATP-dependent protease, was up-regulated in the mutant. This study indicates that LoiA regulates a variety of virulence-associated genes in S. Typhimurium. The negative regulation of Lon protease by LoiA indicates that LoiA can regulates several virulence-associated genes in S. Typhimurium via the Lon protease. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/30077369/Global_regulatory_function_of_the_low_oxygen_induced_transcriptional_regulator_LoiA_in_Salmonella_Typhimurium_revealed_by_RNA_sequencing_ DB - PRIME DP - Unbound Medicine ER -