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Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury.
J Integr Med. 2018 09; 16(5):342-349.JI

Abstract

OBJECTIVE

Vigna subterranea is widely consumed as a traditional staple food in Nigeria and some West African countries. The ethanolic seed extract of V. subterranea (EEVS) was investigated for its gastroprotective effects on aspirin plus pylorus ligation-induced gastric ulcerated rats using an in vivo assay.

METHODS

Gastric mucosal ulceration was induced experimentally in Groups 2 to 5 using aspirin plus pylorus ligation. Rats in Group 1 were orally pretreated with 3% Tween 80 only as normal control. Groups 2 to 5 were pretreated with 3% Tween 80 (ulcer group), 20 mg/kg of omeprazole (positive group), and 200 and 400 mg/kg of EEVS (experimental groups), respectively, once daily for 21 days before ulcer induction. Parameters including those for gastric secretions, ulcerated areas and gastric wall histology were assessed. Levels of superoxide dismutase (SOD), glutathione peroxidase (GPX), and malondialdehyde (MDA) in the gastric tissue homogenate were also determined.

RESULTS

Pretreatment with EEVS significantly (P < 0.05) reduced the ulcer index, gastric volume and total acidity in rats with aspirin plus pylorus ligation-induced ulcer. The pH and mucus of gastric content increased significantly (P < 0.05) while the levels of SOD and GPX were observed to be elevated with a reduced amount of MDA. Significant severe gastric mucosal injury was exhibited in the ulcer group and EEVS or omeprazole offered significant (P < 0.05) protection against mucosal ulceration. Histologically, the gastric submucosal layer showed remarkable decrease in edema and leucocytes infiltration compared with ulcer group.

CONCLUSION

The study suggests that EEVS offered a protective action against aspirin plus pylorus ligation-induced gastric ulcers in Wistar rats. The protective effect might be mediated via antisecretory, cytoprotective and antioxidative mechanisms.

Authors+Show Affiliations

Department of Physiology, Faculty of Medicine, Ebonyi State University, Ebonyi State, Abakaliki 480214, Nigeria. Electronic address: marufbalogun@yahoo.com.Department of Physiology, Faculty of Medicine, Ebonyi State University, Ebonyi State, Abakaliki 480214, Nigeria.Department of Physiology, Faculty of Medicine, Ebonyi State University, Ebonyi State, Abakaliki 480214, Nigeria.Department of Physiology, Faculty of Medicine, Ebonyi State University, Ebonyi State, Abakaliki 480214, Nigeria; Clinical Services Department, National Obstetrics Fistula Centre, Ebonyi State, Abakaliki 480214, Nigeria.Department of Physiology, Faculty of Medicine, Ebonyi State University, Ebonyi State, Abakaliki 480214, Nigeria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30077645

Citation

Balogun, Morufu Eyitayo, et al. "Protective Roles of Vigna Subterranea (Bambara Nut) in Rats With Aspirin-induced Gastric Mucosal Injury." Journal of Integrative Medicine, vol. 16, no. 5, 2018, pp. 342-349.
Balogun ME, Besong EE, Obimma JN, et al. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. J Integr Med. 2018;16(5):342-349.
Balogun, M. E., Besong, E. E., Obimma, J. N., Mbamalu, O. S., & Djobissie, F. S. A. (2018). Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 16(5), 342-349. https://doi.org/10.1016/j.joim.2018.07.004
Balogun ME, et al. Protective Roles of Vigna Subterranea (Bambara Nut) in Rats With Aspirin-induced Gastric Mucosal Injury. J Integr Med. 2018;16(5):342-349. PubMed PMID: 30077645.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. AU - Balogun,Morufu Eyitayo, AU - Besong,Elizabeth Enohnyaket, AU - Obimma,Jacinta Nkechi, AU - Mbamalu,Ogochukwu Sophia, AU - Djobissie,Fankou Serges Athanase, Y1 - 2018/08/01/ PY - 2018/01/06/received PY - 2018/05/06/accepted PY - 2018/8/6/pubmed PY - 2018/12/18/medline PY - 2018/8/6/entrez KW - Anti-oxidative KW - Aspirin plus pylorus ligation KW - Gastric ulcers KW - Rats KW - Vigna subterranea SP - 342 EP - 349 JF - Journal of integrative medicine JO - J Integr Med VL - 16 IS - 5 N2 - OBJECTIVE: Vigna subterranea is widely consumed as a traditional staple food in Nigeria and some West African countries. The ethanolic seed extract of V. subterranea (EEVS) was investigated for its gastroprotective effects on aspirin plus pylorus ligation-induced gastric ulcerated rats using an in vivo assay. METHODS: Gastric mucosal ulceration was induced experimentally in Groups 2 to 5 using aspirin plus pylorus ligation. Rats in Group 1 were orally pretreated with 3% Tween 80 only as normal control. Groups 2 to 5 were pretreated with 3% Tween 80 (ulcer group), 20 mg/kg of omeprazole (positive group), and 200 and 400 mg/kg of EEVS (experimental groups), respectively, once daily for 21 days before ulcer induction. Parameters including those for gastric secretions, ulcerated areas and gastric wall histology were assessed. Levels of superoxide dismutase (SOD), glutathione peroxidase (GPX), and malondialdehyde (MDA) in the gastric tissue homogenate were also determined. RESULTS: Pretreatment with EEVS significantly (P < 0.05) reduced the ulcer index, gastric volume and total acidity in rats with aspirin plus pylorus ligation-induced ulcer. The pH and mucus of gastric content increased significantly (P < 0.05) while the levels of SOD and GPX were observed to be elevated with a reduced amount of MDA. Significant severe gastric mucosal injury was exhibited in the ulcer group and EEVS or omeprazole offered significant (P < 0.05) protection against mucosal ulceration. Histologically, the gastric submucosal layer showed remarkable decrease in edema and leucocytes infiltration compared with ulcer group. CONCLUSION: The study suggests that EEVS offered a protective action against aspirin plus pylorus ligation-induced gastric ulcers in Wistar rats. The protective effect might be mediated via antisecretory, cytoprotective and antioxidative mechanisms. SN - 2095-4964 UR - https://www.unboundmedicine.com/medline/citation/30077645/Protective_roles_of_Vigna_subterranea__Bambara_nut__in_rats_with_aspirin_induced_gastric_mucosal_injury_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S2095-4964(18)30080-3 DB - PRIME DP - Unbound Medicine ER -