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Purple pitanga fruit (Eugenia uniflora L.) protects against oxidative stress and increase the lifespan in Caenorhabditis elegans via the DAF-16/FOXO pathway.
Food Chem Toxicol 2018; 120:639-650FC

Abstract

Pitanga, a fruit of the pitangueira tree (Eugenia uniflora L.), is native to Brazil and has a high antioxidant capacity due to the elevated amount of anthocyanins. The present study aimed to investigate the chemical composition of the purple pitanga fruit and to evaluate its antioxidant effect in the nematode Caenorhabditis elegans. We observed that the ethanolic extract of purple pitanga did not cause any toxic effects but notably increased worm lifespan. The extract improved the survival, reproduction and lifespan of the worms in pre- and post-exposure to stressors H2O2 and juglone, as well as improved the lifespan of the oxidative stress hypersensitive strain mev-1. Notably, PPE extract decreased reactive oxygen species by DCF-DA probe and protein carbonyl content from worms stressed with H2O2. The extract also affected the expression of superoxide dismutase SOD-3 and heat shock protein HSP-16.2 levels, daf 16 target genes that modulate lifespan and antioxidant metabolism. In addition, we demonstrate that these effects are dependent on DAF-16, as PPE extract did not provide protection in daf-16 mutants. Therefore, these results suggest that PPE significantly protected against oxidative stress modulating daf-16 target genes.

Authors+Show Affiliations

Universidade Federal Do Pampa, Campus Uruguaiana, BR 472, Km 592, Caixa Postal 118, Uruguaiana, RS, Brazil.Universidade Federal Do Pampa, Campus Uruguaiana, BR 472, Km 592, Caixa Postal 118, Uruguaiana, RS, Brazil.Universidade Federal Do Pampa, Campus Uruguaiana, BR 472, Km 592, Caixa Postal 118, Uruguaiana, RS, Brazil.Universidade Federal Do Pampa, Campus Uruguaiana, BR 472, Km 592, Caixa Postal 118, Uruguaiana, RS, Brazil.Universidade Federal Do Pampa, Campus Uruguaiana, BR 472, Km 592, Caixa Postal 118, Uruguaiana, RS, Brazil.Universidade Federal Do Pampa, Campus Uruguaiana, BR 472, Km 592, Caixa Postal 118, Uruguaiana, RS, Brazil.Department of Food Science, Faculty of Food Engineering, University of Campinas (UNICAMP), 13083-162, Campinas, Brazil.Department of Food Science, Faculty of Food Engineering, University of Campinas (UNICAMP), 13083-162, Campinas, Brazil.Universidade Federal Do Pampa, Campus Uruguaiana, BR 472, Km 592, Caixa Postal 118, Uruguaiana, RS, Brazil.Universidade Federal Do Pampa, Campus Uruguaiana, BR 472, Km 592, Caixa Postal 118, Uruguaiana, RS, Brazil. Electronic address: cristianedenardin@unipampa.edu.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30077708

Citation

Tambara, Andréia Limana, et al. "Purple Pitanga Fruit (Eugenia Uniflora L.) Protects Against Oxidative Stress and Increase the Lifespan in Caenorhabditis Elegans Via the DAF-16/FOXO Pathway." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 120, 2018, pp. 639-650.
Tambara AL, de Los Santos Moraes L, Dal Forno AH, et al. Purple pitanga fruit (Eugenia uniflora L.) protects against oxidative stress and increase the lifespan in Caenorhabditis elegans via the DAF-16/FOXO pathway. Food Chem Toxicol. 2018;120:639-650.
Tambara, A. L., de Los Santos Moraes, L., Dal Forno, A. H., Boldori, J. R., Gonçalves Soares, A. T., de Freitas Rodrigues, C., ... Denardin, C. C. (2018). Purple pitanga fruit (Eugenia uniflora L.) protects against oxidative stress and increase the lifespan in Caenorhabditis elegans via the DAF-16/FOXO pathway. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 120, pp. 639-650. doi:10.1016/j.fct.2018.07.057.
Tambara AL, et al. Purple Pitanga Fruit (Eugenia Uniflora L.) Protects Against Oxidative Stress and Increase the Lifespan in Caenorhabditis Elegans Via the DAF-16/FOXO Pathway. Food Chem Toxicol. 2018;120:639-650. PubMed PMID: 30077708.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purple pitanga fruit (Eugenia uniflora L.) protects against oxidative stress and increase the lifespan in Caenorhabditis elegans via the DAF-16/FOXO pathway. AU - Tambara,Andréia Limana, AU - de Los Santos Moraes,Liliana, AU - Dal Forno,Ana Helena, AU - Boldori,Jean Ramos, AU - Gonçalves Soares,Ana Thalita, AU - de Freitas Rodrigues,Cristiane, AU - Mariutti,Lilian Regina Barros, AU - Mercadante,Adriana Zerlotti, AU - de Ávila,Daiana Silva, AU - Denardin,Cristiane Casagrande, Y1 - 2018/08/02/ PY - 2018/03/15/received PY - 2018/07/19/revised PY - 2018/07/30/accepted PY - 2018/8/6/pubmed PY - 2018/12/12/medline PY - 2018/8/6/entrez KW - Aging KW - Antioxidants KW - Berries KW - Oxidative stress SP - 639 EP - 650 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem. Toxicol. VL - 120 N2 - Pitanga, a fruit of the pitangueira tree (Eugenia uniflora L.), is native to Brazil and has a high antioxidant capacity due to the elevated amount of anthocyanins. The present study aimed to investigate the chemical composition of the purple pitanga fruit and to evaluate its antioxidant effect in the nematode Caenorhabditis elegans. We observed that the ethanolic extract of purple pitanga did not cause any toxic effects but notably increased worm lifespan. The extract improved the survival, reproduction and lifespan of the worms in pre- and post-exposure to stressors H2O2 and juglone, as well as improved the lifespan of the oxidative stress hypersensitive strain mev-1. Notably, PPE extract decreased reactive oxygen species by DCF-DA probe and protein carbonyl content from worms stressed with H2O2. The extract also affected the expression of superoxide dismutase SOD-3 and heat shock protein HSP-16.2 levels, daf 16 target genes that modulate lifespan and antioxidant metabolism. In addition, we demonstrate that these effects are dependent on DAF-16, as PPE extract did not provide protection in daf-16 mutants. Therefore, these results suggest that PPE significantly protected against oxidative stress modulating daf-16 target genes. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/30077708/Purple_pitanga_fruit__Eugenia_uniflora_L___protects_against_oxidative_stress_and_increase_the_lifespan_in_Caenorhabditis_elegans_via_the_DAF_16/FOXO_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(18)30502-7 DB - PRIME DP - Unbound Medicine ER -