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Organ-specific and genotype-dependent constitutive biosynthesis of secoiridoid glucosides in Centaurium erythraea Rafn, and its elicitation with methyl jasmonate.
Phytochemistry. 2018 Nov; 155:69-82.P

Abstract

While bioactive properties of Centaurium erythraea Rafn secoiridoid glucosides (SG) are widely recognized, many aspects related to their biochemistry, metabolism and relationship to the overall plant physiology are not yet understood. Here we present for the first time an insight into the molecular background of organ-specific and genotype-dependent constitutive biosynthesis of secoiridoids in C. erythraea, by comparing chemical profiles and secoiridoid glucosides-related gene expression. Genes encoding enzymes for intermediate steps of secoiridoids biosynthesis up to secologanin have been identified by analysing transcriptomic data from C. erythraea leaves. Results suggest an organ-specific capacity for the production and accumulation of secoiridoid glucosides, and highlight leaves as the main biosynthesis site. They also point out that significant differences in SG content among various C. erythraea genotypes, are, at least partially, determined by different expression patterns of SG-related genes. The biosynthesis of SG in C. erythraea leaves is enhanced upon treatments with methyl jasmonate (MeJA), which causes reprogramming of SG-related gene expression, leading to an increased production of valuable bioactive compounds. The present study unveiled several rate-limiting genes (encoding GES, G8O, 8HGO, IS and 7DLGT) in SG biosynthesis. SLS and CPR are highlighted as important genes/enzymes that might regulate biosynthetic flux through SG pathway. Information gathered within this study will help us gain deeper insight into the SG metabolism and develop strategies for enhanced biosynthesis of specific secoiridoid glucosides in homologous or heterologous systems.

Authors+Show Affiliations

Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia. Electronic address: dragana.bozic@ibiss.bg.ac.rs.Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia; Faculty of Biology, University of Belgrade, Takovska 43, 11060 Belgrade, Serbia.Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.Faculty of Biology, University of Belgrade, Takovska 43, 11060 Belgrade, Serbia.Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30077897

Citation

Matekalo, Dragana, et al. "Organ-specific and Genotype-dependent Constitutive Biosynthesis of Secoiridoid Glucosides in Centaurium Erythraea Rafn, and Its Elicitation With Methyl Jasmonate." Phytochemistry, vol. 155, 2018, pp. 69-82.
Matekalo D, Skorić M, Nikolić T, et al. Organ-specific and genotype-dependent constitutive biosynthesis of secoiridoid glucosides in Centaurium erythraea Rafn, and its elicitation with methyl jasmonate. Phytochemistry. 2018;155:69-82.
Matekalo, D., Skorić, M., Nikolić, T., Novaković, L., Lukić, M., Božunović, J., Aničić, N., Filipović, B., & Mišić, D. (2018). Organ-specific and genotype-dependent constitutive biosynthesis of secoiridoid glucosides in Centaurium erythraea Rafn, and its elicitation with methyl jasmonate. Phytochemistry, 155, 69-82. https://doi.org/10.1016/j.phytochem.2018.07.015
Matekalo D, et al. Organ-specific and Genotype-dependent Constitutive Biosynthesis of Secoiridoid Glucosides in Centaurium Erythraea Rafn, and Its Elicitation With Methyl Jasmonate. Phytochemistry. 2018;155:69-82. PubMed PMID: 30077897.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Organ-specific and genotype-dependent constitutive biosynthesis of secoiridoid glucosides in Centaurium erythraea Rafn, and its elicitation with methyl jasmonate. AU - Matekalo,Dragana, AU - Skorić,Marijana, AU - Nikolić,Tijana, AU - Novaković,Lazar, AU - Lukić,Milana, AU - Božunović,Jelena, AU - Aničić,Neda, AU - Filipović,Biljana, AU - Mišić,Danijela, Y1 - 2018/08/02/ PY - 2018/04/05/received PY - 2018/06/20/revised PY - 2018/07/24/accepted PY - 2018/8/6/pubmed PY - 2018/11/6/medline PY - 2018/8/6/entrez KW - Centaurium erythraea Rafn KW - Centaury KW - Gentianaceae KW - Methyl jasmonate KW - Secoiridoid pathway regulation KW - Secoiridoids SP - 69 EP - 82 JF - Phytochemistry JO - Phytochemistry VL - 155 N2 - While bioactive properties of Centaurium erythraea Rafn secoiridoid glucosides (SG) are widely recognized, many aspects related to their biochemistry, metabolism and relationship to the overall plant physiology are not yet understood. Here we present for the first time an insight into the molecular background of organ-specific and genotype-dependent constitutive biosynthesis of secoiridoids in C. erythraea, by comparing chemical profiles and secoiridoid glucosides-related gene expression. Genes encoding enzymes for intermediate steps of secoiridoids biosynthesis up to secologanin have been identified by analysing transcriptomic data from C. erythraea leaves. Results suggest an organ-specific capacity for the production and accumulation of secoiridoid glucosides, and highlight leaves as the main biosynthesis site. They also point out that significant differences in SG content among various C. erythraea genotypes, are, at least partially, determined by different expression patterns of SG-related genes. The biosynthesis of SG in C. erythraea leaves is enhanced upon treatments with methyl jasmonate (MeJA), which causes reprogramming of SG-related gene expression, leading to an increased production of valuable bioactive compounds. The present study unveiled several rate-limiting genes (encoding GES, G8O, 8HGO, IS and 7DLGT) in SG biosynthesis. SLS and CPR are highlighted as important genes/enzymes that might regulate biosynthetic flux through SG pathway. Information gathered within this study will help us gain deeper insight into the SG metabolism and develop strategies for enhanced biosynthesis of specific secoiridoid glucosides in homologous or heterologous systems. SN - 1873-3700 UR - https://www.unboundmedicine.com/medline/citation/30077897/Organ_specific_and_genotype_dependent_constitutive_biosynthesis_of_secoiridoid_glucosides_in_Centaurium_erythraea_Rafn_and_its_elicitation_with_methyl_jasmonate_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0031-9422(18)30377-7 DB - PRIME DP - Unbound Medicine ER -