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Use of ultrasound irradiation to inactivate Cryptosporidium parvum oocysts in effluents from municipal wastewater treatment plants.
Ultrason Sonochem. 2018 Nov; 48:118-126.US

Abstract

Water reuse is currently considered an innovative way to addressing water shortage that can provide significant economic, social and environmental benefits, particularly -but not exclusively- in water deficient areas. The potential transmission of infectious diseases is the most common concern in relation to water reclamation. Cryptosporidium is an important genus of protozoan enteropathogens that infect a wide range of vertebrate hosts, including humans. The infective form (oocyst) is highly resistant to the environmental conditions and disinfection treatments. Consequently, Cryptosporidium is the most common etiological agent identified in waterborne outbreaks attributed to parasitic protozoa worldwide. The present study evaluates the efficacy of ultrasound disinfection, at three power levels (60, 80 and 100 W), pulsed at 50% or in continuous mode, for inactivating the waterborne protozoan parasite Cryptosporidium parvum in simulated and real effluents from municipal wastewater treatment plants (MWTPs). Overall interpretation of the results shows that the application of ultrasound irradiation at 80 W power in continuous mode for an exposure time of 10 min drastically reduced the viability of C. parvum. Thus, oocyst viabilities of 4.16 ± 1.93%; 1.29 ± 0.86%; 3.16 ± 0.69%; and 3.15 ± 0.87% were obtained in distilled water, simulated, real and filtered MWTP effluents, respectively (vs 98.57 ± 0.01%, initial oocyst viability), as determined using inclusion/exclusion of the fluorogenic vital dye propidium iodide, an indicator of the integrity of the oocyst wall. Independently of the mode used (pulsed/continuous) and at 80 W power, higher level of oocyst inactivation was detected in MWTP effluents than in distilled water used as a control solution, may be due to the differences in the chemical composition of the samples. Comparison of the results obtained in both modes showed that use of the continuous mode yielded significantly lower oocyst viability. However, when the Dose parameter was considered (energy per volume unit), no statistically significant differences in oocyst viability were observed in relation to the type of mode used. The results demonstrate that ultrasound technology represents a promising alternative to the disinfection methods (ultraviolet irradiation and chlorine products) currently used in water reclamation as it drastically reduces the survival of Cryptosporidium oocysts, without changing the chemical composition of the water or producing toxic by-products.

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Authors+Show Affiliations

Abeledo-Lameiro MJ
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, Campus Vida, 15782 Santiago de Compostela, A Coruña, Spain.
Ares-Mazás E
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, Campus Vida, 15782 Santiago de Compostela, A Coruña, Spain.
Goméz-Couso H
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, Campus Vida, 15782 Santiago de Compostela, A Coruña, Spain; Institute of Food Research and Analysis, University of Santiago de Compostela, Campus Vida, 15782 Santiago de Compostela, A Coruña, Spain. Electronic address: hipolito.gomez@usc.es.

MeSH

CitiesCryptosporidium parvumOocystsUltrasonic WavesWastewaterWater Purification

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30080534

Citation

Abeledo-Lameiro, María Jesús, et al. "Use of Ultrasound Irradiation to Inactivate Cryptosporidium Parvum Oocysts in Effluents From Municipal Wastewater Treatment Plants." Ultrasonics Sonochemistry, vol. 48, 2018, pp. 118-126.
Abeledo-Lameiro MJ, Ares-Mazás E, Goméz-Couso H. Use of ultrasound irradiation to inactivate Cryptosporidium parvum oocysts in effluents from municipal wastewater treatment plants. Ultrason Sonochem. 2018;48:118-126.
Abeledo-Lameiro, M. J., Ares-Mazás, E., & Goméz-Couso, H. (2018). Use of ultrasound irradiation to inactivate Cryptosporidium parvum oocysts in effluents from municipal wastewater treatment plants. Ultrasonics Sonochemistry, 48, 118-126. https://doi.org/10.1016/j.ultsonch.2018.05.013
Abeledo-Lameiro MJ, Ares-Mazás E, Goméz-Couso H. Use of Ultrasound Irradiation to Inactivate Cryptosporidium Parvum Oocysts in Effluents From Municipal Wastewater Treatment Plants. Ultrason Sonochem. 2018;48:118-126. PubMed PMID: 30080534.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Use of ultrasound irradiation to inactivate Cryptosporidium parvum oocysts in effluents from municipal wastewater treatment plants. AU - Abeledo-Lameiro,María Jesús, AU - Ares-Mazás,Elvira, AU - Goméz-Couso,Hipólito, Y1 - 2018/05/17/ PY - 2017/11/22/received PY - 2018/04/12/revised PY - 2018/05/15/accepted PY - 2018/8/7/entrez PY - 2018/8/7/pubmed PY - 2018/9/25/medline KW - Cryptosporidium parvum KW - Oocyst inactivation KW - Reclaimed water KW - Ultrasound irradiation KW - Water reuse SP - 118 EP - 126 JF - Ultrasonics sonochemistry JO - Ultrason Sonochem VL - 48 N2 - Water reuse is currently considered an innovative way to addressing water shortage that can provide significant economic, social and environmental benefits, particularly -but not exclusively- in water deficient areas. The potential transmission of infectious diseases is the most common concern in relation to water reclamation. Cryptosporidium is an important genus of protozoan enteropathogens that infect a wide range of vertebrate hosts, including humans. The infective form (oocyst) is highly resistant to the environmental conditions and disinfection treatments. Consequently, Cryptosporidium is the most common etiological agent identified in waterborne outbreaks attributed to parasitic protozoa worldwide. The present study evaluates the efficacy of ultrasound disinfection, at three power levels (60, 80 and 100 W), pulsed at 50% or in continuous mode, for inactivating the waterborne protozoan parasite Cryptosporidium parvum in simulated and real effluents from municipal wastewater treatment plants (MWTPs). Overall interpretation of the results shows that the application of ultrasound irradiation at 80 W power in continuous mode for an exposure time of 10 min drastically reduced the viability of C. parvum. Thus, oocyst viabilities of 4.16 ± 1.93%; 1.29 ± 0.86%; 3.16 ± 0.69%; and 3.15 ± 0.87% were obtained in distilled water, simulated, real and filtered MWTP effluents, respectively (vs 98.57 ± 0.01%, initial oocyst viability), as determined using inclusion/exclusion of the fluorogenic vital dye propidium iodide, an indicator of the integrity of the oocyst wall. Independently of the mode used (pulsed/continuous) and at 80 W power, higher level of oocyst inactivation was detected in MWTP effluents than in distilled water used as a control solution, may be due to the differences in the chemical composition of the samples. Comparison of the results obtained in both modes showed that use of the continuous mode yielded significantly lower oocyst viability. However, when the Dose parameter was considered (energy per volume unit), no statistically significant differences in oocyst viability were observed in relation to the type of mode used. The results demonstrate that ultrasound technology represents a promising alternative to the disinfection methods (ultraviolet irradiation and chlorine products) currently used in water reclamation as it drastically reduces the survival of Cryptosporidium oocysts, without changing the chemical composition of the water or producing toxic by-products. SN - 1873-2828 UR - https://www.unboundmedicine.com/medline/citation/30080534/Use_of_ultrasound_irradiation_to_inactivate_Cryptosporidium_parvum_oocysts_in_effluents_from_municipal_wastewater_treatment_plants_ DB - PRIME DP - Unbound Medicine ER -