Tags

Type your tag names separated by a space and hit enter

Visual and fluorometric lateral flow immunoassay combined with a dual-functional test mode for rapid determination of tetracycline antibiotics.
Mikrochim Acta 2018; 185(9):404MA

Abstract

A fluorometric immunochromatographic assay (FICA) is described where ZnCdSe/ZnS quantum dots (QDs) act as fluorescent label and gold nanoparticles (AuNPs) act as quencher. The assay works in the "turn-on" mode, i.e. the fluorescent signal (best measured at excitation/emission wavelengths of 302/525 nm) increases with the increase of analyte concentration. This assay can detect tetracycline antibiotics including tetracycline, oxytetracycline, chlortetracycline, and doxycycline. It is not interfered by other veterinary drugs. The visual limits of detection (LODs) for the tetracycline antibiotics are 2 μg·L-1 in buffer, 20 μg·L-1 in milk, and 40 μg·kg-1 in animal muscle tissue. The assay (including sample treatment) can be performed within 30 min. The FICA based on "turn on" mode is more sensitive than the colloidal gold-based immunochromatographic assay (CGICA) and quantum dot-based immunochromatographic assay (QDICA) based on "turn off" mode using either AuNPs or QDs as signal labels. One strip can simultaneously provide the fluorescent test results in the "turn on" mode on the basis of QD luminescence quenching under UV light. The colorimetric test is of the "turn off" mode based on the formation of a red coloration due to the use of AuNPs under natural light. The use of such a dual-functional test mode allows for rapid semi-quantitative determination of tetracycline antibiotics in milk and tissue samples. Graphical abstract Schematoc of a fluorometric immunochromatographic assay (FICA) based on fluorescence quenching of quantum dot (QD) by gold nanoparticle (AuNP) combined with a dual-functional test mode under UV light (turn on mode) and natural light (turn off mode) to visually detect tetracycline antibiotics.

Authors+Show Affiliations

State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China. shengweijunchen@163.com.State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China. s.wang@tust.edu.cn. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU), Beijing, 100048, China. s.wang@tust.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30088104

Citation

Sheng, Wei, et al. "Visual and Fluorometric Lateral Flow Immunoassay Combined With a Dual-functional Test Mode for Rapid Determination of Tetracycline Antibiotics." Mikrochimica Acta, vol. 185, no. 9, 2018, p. 404.
Sheng W, Chang Q, Shi Y, et al. Visual and fluorometric lateral flow immunoassay combined with a dual-functional test mode for rapid determination of tetracycline antibiotics. Mikrochim Acta. 2018;185(9):404.
Sheng, W., Chang, Q., Shi, Y., Duan, W., Zhang, Y., & Wang, S. (2018). Visual and fluorometric lateral flow immunoassay combined with a dual-functional test mode for rapid determination of tetracycline antibiotics. Mikrochimica Acta, 185(9), p. 404. doi:10.1007/s00604-018-2945-9.
Sheng W, et al. Visual and Fluorometric Lateral Flow Immunoassay Combined With a Dual-functional Test Mode for Rapid Determination of Tetracycline Antibiotics. Mikrochim Acta. 2018 08 7;185(9):404. PubMed PMID: 30088104.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Visual and fluorometric lateral flow immunoassay combined with a dual-functional test mode for rapid determination of tetracycline antibiotics. AU - Sheng,Wei, AU - Chang,Qing, AU - Shi,Yingjie, AU - Duan,Wenxia, AU - Zhang,Yan, AU - Wang,Shuo, Y1 - 2018/08/07/ PY - 2018/04/30/received PY - 2018/08/02/accepted PY - 2018/8/9/entrez PY - 2018/8/9/pubmed PY - 2019/2/5/medline KW - Antibiotics residues KW - Food safety KW - Gold nanoparticles KW - Immunochromatographic assay KW - On-site detection KW - Quantum dots KW - Semi-quantitative analysis KW - Test strip KW - Unaided eye readout SP - 404 EP - 404 JF - Mikrochimica acta JO - Mikrochim Acta VL - 185 IS - 9 N2 - A fluorometric immunochromatographic assay (FICA) is described where ZnCdSe/ZnS quantum dots (QDs) act as fluorescent label and gold nanoparticles (AuNPs) act as quencher. The assay works in the "turn-on" mode, i.e. the fluorescent signal (best measured at excitation/emission wavelengths of 302/525 nm) increases with the increase of analyte concentration. This assay can detect tetracycline antibiotics including tetracycline, oxytetracycline, chlortetracycline, and doxycycline. It is not interfered by other veterinary drugs. The visual limits of detection (LODs) for the tetracycline antibiotics are 2 μg·L-1 in buffer, 20 μg·L-1 in milk, and 40 μg·kg-1 in animal muscle tissue. The assay (including sample treatment) can be performed within 30 min. The FICA based on "turn on" mode is more sensitive than the colloidal gold-based immunochromatographic assay (CGICA) and quantum dot-based immunochromatographic assay (QDICA) based on "turn off" mode using either AuNPs or QDs as signal labels. One strip can simultaneously provide the fluorescent test results in the "turn on" mode on the basis of QD luminescence quenching under UV light. The colorimetric test is of the "turn off" mode based on the formation of a red coloration due to the use of AuNPs under natural light. The use of such a dual-functional test mode allows for rapid semi-quantitative determination of tetracycline antibiotics in milk and tissue samples. Graphical abstract Schematoc of a fluorometric immunochromatographic assay (FICA) based on fluorescence quenching of quantum dot (QD) by gold nanoparticle (AuNP) combined with a dual-functional test mode under UV light (turn on mode) and natural light (turn off mode) to visually detect tetracycline antibiotics. SN - 1436-5073 UR - https://www.unboundmedicine.com/medline/citation/30088104/Visual_and_fluorometric_lateral_flow_immunoassay_combined_with_a_dual_functional_test_mode_for_rapid_determination_of_tetracycline_antibiotics_ L2 - https://medlineplus.gov/antibiotics.html DB - PRIME DP - Unbound Medicine ER -