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[Electroacupuncture of "Zusanli" (ST 36) Raises Muscular Force by Adjusting AMPK/PGC-1 α Signaling in Rats with Chronic Fatigue Syndrome].
Zhen Ci Yan Jiu. 2018 Jun 25; 43(6):335-40.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) of "Zusanli" (ST 36) on mitochondrial oxidative stress of skeletal muscle in rats with chronic fatigue syndrome (CFS) based on adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK)/ peroxlsome proliferator-activated receptor-γ coactivator-1 α (PGC-1 α) signaling, in order to reveal its mechanism underlying improvement of CFS.

METHODS

Forty SD rats were randomly divided into normal control, CFS model, EA-Zusanli (ST 36) and EA-non-acupoint groups (n=10 rats in each group). The CFS model was established by forced exhausted load-bearing swimming (twice daily), chronic constraint (1 h) and sleep deprivation (20 h/day) for 14 days. Following modeling, EA (2 Hz/100 Hz, 2 V) was applied to bilateral Zusanli (ST 36) or non-acupoint (about 10-15 mm superior to the bilateral Iliac creast and about 20 mm lateral to the posterior median line) for 20 min, once a day for 10 days. The expression levels of ATP synthase, AMPK, phosphorylated (p)-AMPK, silent mating type information regulation 2 homolog-1 (SIRT 1) and PGC-1 α proteins, and ATP synthase, SIRT 1 and PGC-1 α mRNAs of the quadriceps femoris muscle were detected by Western blot and fluorescence quantitative PCR, respectively. The rats' grabbing force was detected by using a grabbing-force detector.

RESULTS

Compared with the normal group, the grabbing force, and the expression levels of ATP synthase and PGC-1 α proteins and mRNAs were significantly decreased (P<0.05, P<0.01), while the expression of SIRT 1 protein was significantly up-regulated (P<0.05) in the CFS model group. Following EA intervention, the grabbing force and the expression levels of ATP synthase mRNA, SIRT 1 and PGC-1 α proteins and mRNAs, and p-AMPK/AMPK were significantly up-regulated in the EA-Zusanli (ST 36) group (P<0.05, P<0.01).

CONCLUSION

EA of ST 36 can raise the grabbing force of CFS rats, which may be related to its effects in up-regulating the expression of ATP synthase mRNA, SIRT 1 and PGC-1 α proteins and mRNAs, and p-AMPK/AMPK to reduce mitochondrial oxidative stress reaction and in increasing ATP synthesis.

Authors+Show Affiliations

Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China.Liaoning Cancer Hospital, Shenyang 110042.Shunde Hospital for Memorizing Wu Zhong-pei, Foshan 528333, Guangdong Province.Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China.Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China.Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China.Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

30091537

Citation

Dong, Jia-Zi, et al. "[Electroacupuncture of "Zusanli" (ST 36) Raises Muscular Force By Adjusting AMPK/PGC-1 Α Signaling in Rats With Chronic Fatigue Syndrome]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 43, no. 6, 2018, pp. 335-40.
Dong JZ, Wei YT, Xu HY, et al. [Electroacupuncture of "Zusanli" (ST 36) Raises Muscular Force by Adjusting AMPK/PGC-1 α Signaling in Rats with Chronic Fatigue Syndrome]. Zhen Ci Yan Jiu. 2018;43(6):335-40.
Dong, J. Z., Wei, Y. T., Xu, H. Y., Zhang, Y., Yong, R. L., Xue, Y. N., & Zhang, L. D. (2018). [Electroacupuncture of "Zusanli" (ST 36) Raises Muscular Force by Adjusting AMPK/PGC-1 α Signaling in Rats with Chronic Fatigue Syndrome]. Zhen Ci Yan Jiu = Acupuncture Research, 43(6), 335-40. https://doi.org/10.13702/j.1000-0607.171010
Dong JZ, et al. [Electroacupuncture of "Zusanli" (ST 36) Raises Muscular Force By Adjusting AMPK/PGC-1 Α Signaling in Rats With Chronic Fatigue Syndrome]. Zhen Ci Yan Jiu. 2018 Jun 25;43(6):335-40. PubMed PMID: 30091537.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Electroacupuncture of "Zusanli" (ST 36) Raises Muscular Force by Adjusting AMPK/PGC-1 α Signaling in Rats with Chronic Fatigue Syndrome]. AU - Dong,Jia-Zi, AU - Wei,Yun-Tao, AU - Xu,Huan-Yu, AU - Zhang,Yu, AU - Yong,Ru-Lin, AU - Xue,Ya-Nan, AU - Zhang,Li-de, PY - 2018/8/10/entrez PY - 2018/8/10/pubmed PY - 2019/4/6/medline KW - AMPK/PGC-1 α signaling KW - Chronic fatigue syndrome KW - Electroacupuncture KW - Grabbing force KW - Mitochondrial oxidative stress KW - Skeletal muscle SP - 335 EP - 40 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 43 IS - 6 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) of "Zusanli" (ST 36) on mitochondrial oxidative stress of skeletal muscle in rats with chronic fatigue syndrome (CFS) based on adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK)/ peroxlsome proliferator-activated receptor-γ coactivator-1 α (PGC-1 α) signaling, in order to reveal its mechanism underlying improvement of CFS. METHODS: Forty SD rats were randomly divided into normal control, CFS model, EA-Zusanli (ST 36) and EA-non-acupoint groups (n=10 rats in each group). The CFS model was established by forced exhausted load-bearing swimming (twice daily), chronic constraint (1 h) and sleep deprivation (20 h/day) for 14 days. Following modeling, EA (2 Hz/100 Hz, 2 V) was applied to bilateral Zusanli (ST 36) or non-acupoint (about 10-15 mm superior to the bilateral Iliac creast and about 20 mm lateral to the posterior median line) for 20 min, once a day for 10 days. The expression levels of ATP synthase, AMPK, phosphorylated (p)-AMPK, silent mating type information regulation 2 homolog-1 (SIRT 1) and PGC-1 α proteins, and ATP synthase, SIRT 1 and PGC-1 α mRNAs of the quadriceps femoris muscle were detected by Western blot and fluorescence quantitative PCR, respectively. The rats' grabbing force was detected by using a grabbing-force detector. RESULTS: Compared with the normal group, the grabbing force, and the expression levels of ATP synthase and PGC-1 α proteins and mRNAs were significantly decreased (P<0.05, P<0.01), while the expression of SIRT 1 protein was significantly up-regulated (P<0.05) in the CFS model group. Following EA intervention, the grabbing force and the expression levels of ATP synthase mRNA, SIRT 1 and PGC-1 α proteins and mRNAs, and p-AMPK/AMPK were significantly up-regulated in the EA-Zusanli (ST 36) group (P<0.05, P<0.01). CONCLUSION: EA of ST 36 can raise the grabbing force of CFS rats, which may be related to its effects in up-regulating the expression of ATP synthase mRNA, SIRT 1 and PGC-1 α proteins and mRNAs, and p-AMPK/AMPK to reduce mitochondrial oxidative stress reaction and in increasing ATP synthesis. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/30091537/[Electroacupuncture_of_"Zusanli"__ST_36__Raises_Muscular_Force_by_Adjusting_AMPK/PGC_1_α_Signaling_in_Rats_with_Chronic_Fatigue_Syndrome]_ L2 - https://medlineplus.gov/chronicfatiguesyndrome.html DB - PRIME DP - Unbound Medicine ER -