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[Effect of Electroacupuncture on Hippocampal IL-6, IL-1 β, TNF-α and Norepinephrine Levels in Acute Myocardial Ischemia Rats].
Zhen Ci Yan Jiu. 2018 Jun 25; 43(6):365-9.ZC

Abstract

OBJECTIVE

We have repeatedly demonstrated that electroacupuncture (EA) stimulation of "Shenmen" (HT 7)- "Tongli" (HT 5) segment of the Heart Meridian can improve acute myocardial ischemia (AMI). This study aimed at observing the effect of EA on contents of hippocampal norepinephrine (NE), and interleukin 6 (IL-6), IL-1 β and tumor necrosis factor- α (TNF-α) in AMI rats, so as to explore its underlying mechanism.

METHODS

SD rats were randomly divided into 3 groups: sham operation (sham), model and EA(n=6 rats in each). The anterior descending branch (ADB) of the left coronary artery was occluded to make an AMI model. For rats of the sham group, a surgical suture was simply threaded beneath the ADB without ligation. EA (2 Hz/15 Hz, 1 mA) was applied to bilateral "Shenmen" (HT 7)- "Tongli" (HT 5) and the middle-point between HT 7 and HT 5 for 30 min, once daily for 3 days. Electrocardiogram (ECG) of the neck-thoracic lead was recorded by using PowerLab 16. The contents of serum creatine kinase (CK), hippocampal IL-6, IL-1 β and TNF-α were assayed by ELISA. The concentration of NE in hippocampal CA 1 area was detected by microdialysis combined with electrochemical detector.

RESULTS

Compared with the sham group, the ECG-ST height, serum CK, hippocampal NE, IL-6, IL-1 β and TNF-α contents of the CA 1 region were significantly increased in the model group (P<0.001). Whereas, after EA intervention, the serum CK, hippocampal NE, IL-6, IL-1 β and TNF-α contents were obviously down-regulated relevant to the model group (P<0.05, P<0.001), and the IL-6, IL-1 β and TNF-α contents were positively correlated with the NE level (P<0.001, P<0.01).

CONCLUSION

EA stimulation of the Heart Meridian may improve ischemic myocardial injury in AMI rats, probably by reducing the proinflammatory factors and hippocampal NE.

Authors+Show Affiliations

Graduate School, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.Key Laboratory of Xin'an Medicine of the Ministry of Education, Anhui University of Traditional Chinese Medicine, Hefei 230038, China; Anhui Provincial Key Laboratory for Cultivating Foundation and Technology of Acupuncture and Moxibustion, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.School of Acupuncture and Rehabilitation Medicine, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006.Graduate School, 2Key Laboratory of Xin'an Medicine of the Ministry of Education, 3Anhui Provincial Key Laboratory for Cultivating Foundation and Technology of Acupuncture and Moxibustion, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.Graduate School, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.Key Laboratory of Xin'an Medicine of the Ministry of Education, Anhui University of Traditional Chinese Medicine, Hefei 230038, China; Anhui Provincial Key Laboratory for Cultivating Foundation and Technology of Acupuncture and Moxibustion, Anhui University of Traditional Chinese Medicine, Hefei 230038, China; Institute of Chinese Medicine in Bozhou, Anhui Academy of Chinese Medicine, Anhui Province, BoZhou 236800.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

30091542

Citation

Wang, Kun, et al. "[Effect of Electroacupuncture On Hippocampal IL-6, IL-1 Β, TNF-α and Norepinephrine Levels in Acute Myocardial Ischemia Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 43, no. 6, 2018, pp. 365-9.
Wang K, Wu SB, Cui S, et al. [Effect of Electroacupuncture on Hippocampal IL-6, IL-1 β, TNF-α and Norepinephrine Levels in Acute Myocardial Ischemia Rats]. Zhen Ci Yan Jiu. 2018;43(6):365-9.
Wang, K., Wu, S. B., Cui, S., Xiang, S. Y., Wu, X., & Zhou, M. Q. (2018). [Effect of Electroacupuncture on Hippocampal IL-6, IL-1 β, TNF-α and Norepinephrine Levels in Acute Myocardial Ischemia Rats]. Zhen Ci Yan Jiu = Acupuncture Research, 43(6), 365-9. https://doi.org/10.13702/j.1000-0607.170972
Wang K, et al. [Effect of Electroacupuncture On Hippocampal IL-6, IL-1 Β, TNF-α and Norepinephrine Levels in Acute Myocardial Ischemia Rats]. Zhen Ci Yan Jiu. 2018 Jun 25;43(6):365-9. PubMed PMID: 30091542.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of Electroacupuncture on Hippocampal IL-6, IL-1 β, TNF-α and Norepinephrine Levels in Acute Myocardial Ischemia Rats]. AU - Wang,Kun, AU - Wu,Sheng-Bing, AU - Cui,Shuai, AU - Xiang,Shui-Ying, AU - Wu,Xin, AU - Zhou,Mei-Qi, PY - 2018/8/10/entrez PY - 2018/8/10/pubmed PY - 2019/4/6/medline KW - Acute myocardial ischemia KW - Electroacupuncture KW - Norepinephrine KW - Proinflammatory factors SP - 365 EP - 9 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 43 IS - 6 N2 - OBJECTIVE: We have repeatedly demonstrated that electroacupuncture (EA) stimulation of "Shenmen" (HT 7)- "Tongli" (HT 5) segment of the Heart Meridian can improve acute myocardial ischemia (AMI). This study aimed at observing the effect of EA on contents of hippocampal norepinephrine (NE), and interleukin 6 (IL-6), IL-1 β and tumor necrosis factor- α (TNF-α) in AMI rats, so as to explore its underlying mechanism. METHODS: SD rats were randomly divided into 3 groups: sham operation (sham), model and EA(n=6 rats in each). The anterior descending branch (ADB) of the left coronary artery was occluded to make an AMI model. For rats of the sham group, a surgical suture was simply threaded beneath the ADB without ligation. EA (2 Hz/15 Hz, 1 mA) was applied to bilateral "Shenmen" (HT 7)- "Tongli" (HT 5) and the middle-point between HT 7 and HT 5 for 30 min, once daily for 3 days. Electrocardiogram (ECG) of the neck-thoracic lead was recorded by using PowerLab 16. The contents of serum creatine kinase (CK), hippocampal IL-6, IL-1 β and TNF-α were assayed by ELISA. The concentration of NE in hippocampal CA 1 area was detected by microdialysis combined with electrochemical detector. RESULTS: Compared with the sham group, the ECG-ST height, serum CK, hippocampal NE, IL-6, IL-1 β and TNF-α contents of the CA 1 region were significantly increased in the model group (P<0.001). Whereas, after EA intervention, the serum CK, hippocampal NE, IL-6, IL-1 β and TNF-α contents were obviously down-regulated relevant to the model group (P<0.05, P<0.001), and the IL-6, IL-1 β and TNF-α contents were positively correlated with the NE level (P<0.001, P<0.01). CONCLUSION: EA stimulation of the Heart Meridian may improve ischemic myocardial injury in AMI rats, probably by reducing the proinflammatory factors and hippocampal NE. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/30091542/[Effect_of_Electroacupuncture_on_Hippocampal_IL_6_IL_1_β_TNF_α_and_Norepinephrine_Levels_in_Acute_Myocardial_Ischemia_Rats]_ DB - PRIME DP - Unbound Medicine ER -