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Developing New Oligo Probes to Distinguish Specific Chromosomal Segments and the A, B, D Genomes of Wheat (Triticum aestivum L.) Using ND-FISH.
Front Plant Sci. 2018; 9:1104.FP

Abstract

Non-denaturing FISH (ND-FISH) technology has been widely used to study the chromosomes of Triticeae species because of its convenience. The oligo probes for ND-FISH analysis of wheat (Triticum aestivum L.) chromosomes are still limited. In this study, the whole genome shotgun assembly sequences (IWGSC WGA v0.4) and the first version of the reference sequences (IWGSC RefSeq v1.0) of Chinese Spring (T. aestivum L.) were used to find new tandem repeats. One hundred and twenty oligo probes were designed according to the new tandem repeats and used for ND-FISH analysis of chromosomes of wheat Chinese Spring. Twenty nine of the 120 oligo probes produce clear or strong signals on wheat chromosomes. Two of the 29 oligo probes can be used to conveniently distinguish wheat A-, B-, and D-genome chromosomes. Sixteen of the 29 oligo probes only produce clear or strong signals on the subtelomeric regions of 1AS, 5AS, 7AL, 4BS, 5BS, and 3DS arms, on the telomeric regions of 1AL, 5AL, 2BS, 3BL, 6DS, and 7DL arms, on the intercalary regions of 4AL and 2DL arms, and on the pericentromeric regions of 3DL and 6DS arms. Eleven of the 29 oligo probes generate distinct signal bands on several chromosomes and they are different from those previously reported. In addition, the short and long arms of 6D chromosome have been confirmed. The new oligo probes developed in this study are useful and convenient for distinguishing wheat chromosomes or specific segments of wheat chromosomes.

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Authors+Show Affiliations

Tang S
Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, China. Institute of Ecological Agriculture, Sichuan Agricultural University, Chengdu, China.
Tang Z
Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, China. Institute of Ecological Agriculture, Sichuan Agricultural University, Chengdu, China.
Qiu L
Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, China.
Yang Z
Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, China.
Li G
Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, China.
Lang T
Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, China.
Zhu W
Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, China. Institute of Ecological Agriculture, Sichuan Agricultural University, Chengdu, China.
Zhang J
Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, China.
Fu S
Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30093909

Citation

Tang, Shuyao, et al. "Developing New Oligo Probes to Distinguish Specific Chromosomal Segments and the A, B, D Genomes of Wheat (Triticum Aestivum L.) Using ND-FISH." Frontiers in Plant Science, vol. 9, 2018, p. 1104.
Tang S, Tang Z, Qiu L, et al. Developing New Oligo Probes to Distinguish Specific Chromosomal Segments and the A, B, D Genomes of Wheat (Triticum aestivum L.) Using ND-FISH. Front Plant Sci. 2018;9:1104.
Tang, S., Tang, Z., Qiu, L., Yang, Z., Li, G., Lang, T., Zhu, W., Zhang, J., & Fu, S. (2018). Developing New Oligo Probes to Distinguish Specific Chromosomal Segments and the A, B, D Genomes of Wheat (Triticum aestivum L.) Using ND-FISH. Frontiers in Plant Science, 9, 1104. https://doi.org/10.3389/fpls.2018.01104
Tang S, et al. Developing New Oligo Probes to Distinguish Specific Chromosomal Segments and the A, B, D Genomes of Wheat (Triticum Aestivum L.) Using ND-FISH. Front Plant Sci. 2018;9:1104. PubMed PMID: 30093909.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Developing New Oligo Probes to Distinguish Specific Chromosomal Segments and the A, B, D Genomes of Wheat (Triticum aestivum L.) Using ND-FISH. AU - Tang,Shuyao, AU - Tang,Zongxiang, AU - Qiu,Ling, AU - Yang,Zujun, AU - Li,Guangrong, AU - Lang,Tao, AU - Zhu,Wenqian, AU - Zhang,Jiehong, AU - Fu,Shulan, Y1 - 2018/07/26/ PY - 2018/04/25/received PY - 2018/07/09/accepted PY - 2018/8/11/entrez PY - 2018/8/11/pubmed PY - 2018/8/11/medline KW - ND-FISH KW - chromosome KW - oligo probe KW - tandem repeats KW - wheat SP - 1104 EP - 1104 JF - Frontiers in plant science JO - Front Plant Sci VL - 9 N2 - Non-denaturing FISH (ND-FISH) technology has been widely used to study the chromosomes of Triticeae species because of its convenience. The oligo probes for ND-FISH analysis of wheat (Triticum aestivum L.) chromosomes are still limited. In this study, the whole genome shotgun assembly sequences (IWGSC WGA v0.4) and the first version of the reference sequences (IWGSC RefSeq v1.0) of Chinese Spring (T. aestivum L.) were used to find new tandem repeats. One hundred and twenty oligo probes were designed according to the new tandem repeats and used for ND-FISH analysis of chromosomes of wheat Chinese Spring. Twenty nine of the 120 oligo probes produce clear or strong signals on wheat chromosomes. Two of the 29 oligo probes can be used to conveniently distinguish wheat A-, B-, and D-genome chromosomes. Sixteen of the 29 oligo probes only produce clear or strong signals on the subtelomeric regions of 1AS, 5AS, 7AL, 4BS, 5BS, and 3DS arms, on the telomeric regions of 1AL, 5AL, 2BS, 3BL, 6DS, and 7DL arms, on the intercalary regions of 4AL and 2DL arms, and on the pericentromeric regions of 3DL and 6DS arms. Eleven of the 29 oligo probes generate distinct signal bands on several chromosomes and they are different from those previously reported. In addition, the short and long arms of 6D chromosome have been confirmed. The new oligo probes developed in this study are useful and convenient for distinguishing wheat chromosomes or specific segments of wheat chromosomes. SN - 1664-462X UR - https://www.unboundmedicine.com/medline/citation/30093909/Developing_New_Oligo_Probes_to_Distinguish_Specific_Chromosomal_Segments_and_the_A_B_D_Genomes_of_Wheat__Triticum_aestivum_L___Using_ND_FISH_ DB - PRIME DP - Unbound Medicine ER -
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