Tags

Type your tag names separated by a space and hit enter

Micellar enhanced spectrofluorimetric approach for nanogram detection of certain α1 -blocker drugs: Application in pharmaceutical preparations and human plasma.
Luminescence. 2018 Nov; 33(7):1226-1234.L

Abstract

Alpha1-adrenergic-blocking drugs, namely; alfuzosin hydrochloride (ALF), doxazosin mesylate (DOX) and terazosin hydrochloride (TER) are effective as antihypertensive agents as well as in management of benign prostatic hypertrophy. In this study, a simple, very fast, highly sensitive and cheap technique was optimized for assay of these drugs in pure states and pharmaceutical tablets. The proposed method is dependent on enhancement of the native fluorescence of investigated drugs using the polyoxyethylene 50 stearate (POE50S) micellar system. The method showed excitation at 325, 340 and 250 nm for ALF, DOX and TER, respectively and an emission maxima at 382 nm. The fluorescence intensity-concentration charts of studied drugs were attained utilizing concentration ranges (2.0-60.0 ng mL-1) for DOX and (4.0-100.0 ng mL-1) for ALF and TER with quantitation limits 2.9, 1.6 and 2.5 ng mL-1 for ALF, DOX and TER, respectively. The suggested technique was approved according to International Council for Harmonisation (ICH) standards and the United States Food and Drug Administration (US FDA) bioanalytical method validation and has been effectively applied for assay of these medications in their dosage forms as well as for content uniformity test. The developed procedure was also efficiently applied for determination of these drugs in real human plasma with high accuracy.

Authors+Show Affiliations

Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.Analytical Chemistry Department, Faculty of Pharmacy, Sadat City University, Sadat City, Egypt.Pharmaceutical analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Assiut branch, Assiut, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30094938

Citation

Omar, Mahmoud A., et al. "Micellar Enhanced Spectrofluorimetric Approach for Nanogram Detection of Certain Α1 -blocker Drugs: Application in Pharmaceutical Preparations and Human Plasma." Luminescence : the Journal of Biological and Chemical Luminescence, vol. 33, no. 7, 2018, pp. 1226-1234.
Omar MA, Hammad MA, Salman BI. Micellar enhanced spectrofluorimetric approach for nanogram detection of certain α1 -blocker drugs: Application in pharmaceutical preparations and human plasma. Luminescence. 2018;33(7):1226-1234.
Omar, M. A., Hammad, M. A., & Salman, B. I. (2018). Micellar enhanced spectrofluorimetric approach for nanogram detection of certain α1 -blocker drugs: Application in pharmaceutical preparations and human plasma. Luminescence : the Journal of Biological and Chemical Luminescence, 33(7), 1226-1234. https://doi.org/10.1002/bio.3539
Omar MA, Hammad MA, Salman BI. Micellar Enhanced Spectrofluorimetric Approach for Nanogram Detection of Certain Α1 -blocker Drugs: Application in Pharmaceutical Preparations and Human Plasma. Luminescence. 2018;33(7):1226-1234. PubMed PMID: 30094938.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Micellar enhanced spectrofluorimetric approach for nanogram detection of certain α1 -blocker drugs: Application in pharmaceutical preparations and human plasma. AU - Omar,Mahmoud A, AU - Hammad,Mohamed A, AU - Salman,Baher I, Y1 - 2018/08/09/ PY - 2018/04/10/received PY - 2018/05/22/revised PY - 2018/07/02/accepted PY - 2018/8/11/pubmed PY - 2018/12/19/medline PY - 2018/8/11/entrez KW - Alfuzosin KW - Doxazosin KW - Human plasma KW - Polyoxyethylene 50 stearate KW - Spectrofluorimetry KW - Surfactant KW - Terazosin SP - 1226 EP - 1234 JF - Luminescence : the journal of biological and chemical luminescence JO - Luminescence VL - 33 IS - 7 N2 - Alpha1-adrenergic-blocking drugs, namely; alfuzosin hydrochloride (ALF), doxazosin mesylate (DOX) and terazosin hydrochloride (TER) are effective as antihypertensive agents as well as in management of benign prostatic hypertrophy. In this study, a simple, very fast, highly sensitive and cheap technique was optimized for assay of these drugs in pure states and pharmaceutical tablets. The proposed method is dependent on enhancement of the native fluorescence of investigated drugs using the polyoxyethylene 50 stearate (POE50S) micellar system. The method showed excitation at 325, 340 and 250 nm for ALF, DOX and TER, respectively and an emission maxima at 382 nm. The fluorescence intensity-concentration charts of studied drugs were attained utilizing concentration ranges (2.0-60.0 ng mL-1) for DOX and (4.0-100.0 ng mL-1) for ALF and TER with quantitation limits 2.9, 1.6 and 2.5 ng mL-1 for ALF, DOX and TER, respectively. The suggested technique was approved according to International Council for Harmonisation (ICH) standards and the United States Food and Drug Administration (US FDA) bioanalytical method validation and has been effectively applied for assay of these medications in their dosage forms as well as for content uniformity test. The developed procedure was also efficiently applied for determination of these drugs in real human plasma with high accuracy. SN - 1522-7243 UR - https://www.unboundmedicine.com/medline/citation/30094938/Micellar_enhanced_spectrofluorimetric_approach_for_nanogram_detection_of_certain_α1_-blocker_drugs:_Application_in_pharmaceutical_preparations_and_human_plasma L2 - https://doi.org/10.1002/bio.3539 DB - PRIME DP - Unbound Medicine ER -