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[Effect of Electroacupuncture at "Shenmen" (HT 7)- "Tongli" (HT 5) of Heart Meridian on Neuronal Activities in Paraventricular Nucleus of Hypothalamus in Myocardial Ischemia Rats].
Zhen Ci Yan Jiu. 2018 Jul 25; 43(7):406-13.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) stimulation of "Shenmen" (HT 7) - "Tongli" (HT 5) segment of the Heart Meridian on neuronal electrical activities of hypothalamic paraventricular nucleus (PVN) in rats with myocardial ischemia (MI), so as to investigate its possible mechanism underlying improvement of MI.

METHODS

Thirty-two SD rats were randomly divided into sham control, model, HT 7-HT 5 and "Taiyuan" (LU 9)- "Lieque" (LU 7) groups (n=8 in each group). EA preconditioning (2 Hz, 1 V, 20 min) was applied to bilateral HT 7-HT 5 and bilateral LU 9-LU 7, respectively, once everyday for 7 days. The electrical activities of the right PVN region were recorded by the implanted microelectrode array(2×4)and Plexon multi-channel acquisition system. Cluster analysis of neuronal signals was carried out by Offline Sorter software. The discharge waveforms, autocorrelation and cross-correlation of neuronal activities were analyzed by using Neuro Explorer software.

RESULTS

Cluster analysis of neuronal signals showed that 2, 2, 1 and 1 interneuron in the sham, model, HT 7-HT 5, and LU 9-LU 7 groups, and 3 pyramidal neurons in the HT 7-HT 5 were acquired. Cross correlation analysis showed that the SPK 02 a and SPK 02 b neurons of the HT 7-HT 5 group had an inhibitory relationship. The total discharge frequency was significantly increased in the model group relevant to the sham group (P<0.01), and was markedly lower in the HT 7-HT 5 group than in the model group and LU 9-LU 7 group (P<0.01). Real-time spectrum analysis showed that the local field potential spectrum energy of the HT 7-HT 5 group was significantly lower than that of the model group and the LU 9-LU 7 group.

CONCLUSION

EA of HT 7-HT 5 segment of the Heart Meridian can inhibit the electrical activity of interneuron and activate the electrical activity of pyramidal neuron in PVN region, and an inhibitory relationship exists between the interneuron and pyramidal neuron in MI rats, which may be a mechanism of EA in regulating activities of the ischemic heart.

Authors+Show Affiliations

Research Institute of Acupuncture-moxibustion and Meridians, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.Research Institute of Acupuncture-moxibustion and Meridians, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.Research Institute of Acupuncture-moxibustion and Meridians, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.Research Institute of Acupuncture-moxibustion and Meridians, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.Research Institute of Acupuncture-moxibustion and Meridians, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.Research Institute of Acupuncture-moxibustion and Meridians, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.Research Institute of Acupuncture-moxibustion and Meridians, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.Research Institute of Acupuncture-moxibustion and Meridians, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.Research Institute of Acupuncture-moxibustion and Meridians, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

30094975

Citation

Cai, Rong-Lin, et al. "[Effect of Electroacupuncture at "Shenmen" (HT 7)- "Tongli" (HT 5) of Heart Meridian On Neuronal Activities in Paraventricular Nucleus of Hypothalamus in Myocardial Ischemia Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 43, no. 7, 2018, pp. 406-13.
Cai RL, Cui S, Wu ZJ, et al. [Effect of Electroacupuncture at "Shenmen" (HT 7)- "Tongli" (HT 5) of Heart Meridian on Neuronal Activities in Paraventricular Nucleus of Hypothalamus in Myocardial Ischemia Rats]. Zhen Ci Yan Jiu. 2018;43(7):406-13.
Cai, R. L., Cui, S., Wu, Z. J., He, L., Yu, Q., Wang, J., Peng, C. Y., Hu, L., & Zhou, Y. P. (2018). [Effect of Electroacupuncture at "Shenmen" (HT 7)- "Tongli" (HT 5) of Heart Meridian on Neuronal Activities in Paraventricular Nucleus of Hypothalamus in Myocardial Ischemia Rats]. Zhen Ci Yan Jiu = Acupuncture Research, 43(7), 406-13. https://doi.org/10.13702/j.1000-0607.180144
Cai RL, et al. [Effect of Electroacupuncture at "Shenmen" (HT 7)- "Tongli" (HT 5) of Heart Meridian On Neuronal Activities in Paraventricular Nucleus of Hypothalamus in Myocardial Ischemia Rats]. Zhen Ci Yan Jiu. 2018 Jul 25;43(7):406-13. PubMed PMID: 30094975.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of Electroacupuncture at "Shenmen" (HT 7)- "Tongli" (HT 5) of Heart Meridian on Neuronal Activities in Paraventricular Nucleus of Hypothalamus in Myocardial Ischemia Rats]. AU - Cai,Rong-Lin, AU - Cui,Shuai, AU - Wu,Zi-Jian, AU - He,Lu, AU - Yu,Qing, AU - Wang,Jie, AU - Peng,Chuan-Yu, AU - Hu,Ling, AU - Zhou,Yi-Ping, PY - 2018/8/11/entrez PY - 2018/8/11/pubmed PY - 2019/4/6/medline KW - Electroacupuncture KW - Heart Meridian KW - Hypothalamic paraventricular nucleus KW - Microelectrode array KW - Myocardial ischemia KW - Neuronal activity SP - 406 EP - 13 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 43 IS - 7 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) stimulation of "Shenmen" (HT 7) - "Tongli" (HT 5) segment of the Heart Meridian on neuronal electrical activities of hypothalamic paraventricular nucleus (PVN) in rats with myocardial ischemia (MI), so as to investigate its possible mechanism underlying improvement of MI. METHODS: Thirty-two SD rats were randomly divided into sham control, model, HT 7-HT 5 and "Taiyuan" (LU 9)- "Lieque" (LU 7) groups (n=8 in each group). EA preconditioning (2 Hz, 1 V, 20 min) was applied to bilateral HT 7-HT 5 and bilateral LU 9-LU 7, respectively, once everyday for 7 days. The electrical activities of the right PVN region were recorded by the implanted microelectrode array(2×4)and Plexon multi-channel acquisition system. Cluster analysis of neuronal signals was carried out by Offline Sorter software. The discharge waveforms, autocorrelation and cross-correlation of neuronal activities were analyzed by using Neuro Explorer software. RESULTS: Cluster analysis of neuronal signals showed that 2, 2, 1 and 1 interneuron in the sham, model, HT 7-HT 5, and LU 9-LU 7 groups, and 3 pyramidal neurons in the HT 7-HT 5 were acquired. Cross correlation analysis showed that the SPK 02 a and SPK 02 b neurons of the HT 7-HT 5 group had an inhibitory relationship. The total discharge frequency was significantly increased in the model group relevant to the sham group (P<0.01), and was markedly lower in the HT 7-HT 5 group than in the model group and LU 9-LU 7 group (P<0.01). Real-time spectrum analysis showed that the local field potential spectrum energy of the HT 7-HT 5 group was significantly lower than that of the model group and the LU 9-LU 7 group. CONCLUSION: EA of HT 7-HT 5 segment of the Heart Meridian can inhibit the electrical activity of interneuron and activate the electrical activity of pyramidal neuron in PVN region, and an inhibitory relationship exists between the interneuron and pyramidal neuron in MI rats, which may be a mechanism of EA in regulating activities of the ischemic heart. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/30094975/[Effect_of_Electroacupuncture_at_"Shenmen"__HT_7___"Tongli"__HT_5__of_Heart_Meridian_on_Neuronal_Activities_in_Paraventricular_Nucleus_of_Hypothalamus_in_Myocardial_Ischemia_Rats]_ DB - PRIME DP - Unbound Medicine ER -