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Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of polysaccharide from Cynanchum auriculatum Royle ex Wight.
Int J Biol Macromol. 2018 Nov; 119:1068-1076.IJ

Abstract

In the present study, a novel polysaccharide fraction (CAP2-1) from Cynanchum auriculatum Royle ex Wight was obtained by hot water extraction, ethanol precipitation and sequential purification through anion-exchange and size-exclusion chromatography. CAP2-1 was a homogeneous heteropolysaccharide composed of mannose, rhamnose, glucuronic acid, galacturonic acid, galactose and arabinose, with an average molecular weight of 830.93 kDa. FT-IR and NMR spectra of CAP2-1 exhibited typical characteristic signals of polysaccharide. For antioxidant activity evaluation in vitro, CAP2-1 showed effective scavenging capacities against ABTS, DPPH and superoxide anion radical in a dose-dependent manner, with IC50 values at 0.1232, 0.5543 and 0.5881 mg/mL, respectively. At cellular level, CAP2-1 provided a significant protective effect against hydrogen peroxide-induced oxidative stress in HepG2 cells by a compositive oxidation defense mechanism. CAP2-1 could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme SOD and non-enzymatic antioxidant GSH in oxidative damaged cells, in addition to scavenging ROS directly and improving cell viability and membrane integrity, consequently achieving the intracellular antioxidant activity. The results unveiled that CAP2-1 could be explored as a promising natural antioxidant for application in functional foods.

Authors+Show Affiliations

Institute of Farm Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China.Institute of Farm Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China.Institute of Farm Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China.Institute of Farm Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China.College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.Institute of Farm Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China. Electronic address: lichunyang968@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30096394

Citation

Chai, Zhi, et al. "Preparation, Characterization, Antioxidant Activity and Protective Effect Against Cellular Oxidative Stress of Polysaccharide From Cynanchum Auriculatum Royle Ex Wight." International Journal of Biological Macromolecules, vol. 119, 2018, pp. 1068-1076.
Chai Z, Huang W, Zhao X, et al. Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of polysaccharide from Cynanchum auriculatum Royle ex Wight. Int J Biol Macromol. 2018;119:1068-1076.
Chai, Z., Huang, W., Zhao, X., Wu, H., Zeng, X., & Li, C. (2018). Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of polysaccharide from Cynanchum auriculatum Royle ex Wight. International Journal of Biological Macromolecules, 119, 1068-1076. https://doi.org/10.1016/j.ijbiomac.2018.08.024
Chai Z, et al. Preparation, Characterization, Antioxidant Activity and Protective Effect Against Cellular Oxidative Stress of Polysaccharide From Cynanchum Auriculatum Royle Ex Wight. Int J Biol Macromol. 2018;119:1068-1076. PubMed PMID: 30096394.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of polysaccharide from Cynanchum auriculatum Royle ex Wight. AU - Chai,Zhi, AU - Huang,Wuyang, AU - Zhao,Xue, AU - Wu,Han, AU - Zeng,Xiaoxiong, AU - Li,Chunyang, Y1 - 2018/08/07/ PY - 2018/05/08/received PY - 2018/07/22/revised PY - 2018/08/07/accepted PY - 2018/8/11/pubmed PY - 2019/1/1/medline PY - 2018/8/11/entrez KW - Antioxidant activity KW - Characterization KW - Cynanchum auriculatum Royle ex Wight KW - Oxidative stress KW - Polysaccharide SP - 1068 EP - 1076 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 119 N2 - In the present study, a novel polysaccharide fraction (CAP2-1) from Cynanchum auriculatum Royle ex Wight was obtained by hot water extraction, ethanol precipitation and sequential purification through anion-exchange and size-exclusion chromatography. CAP2-1 was a homogeneous heteropolysaccharide composed of mannose, rhamnose, glucuronic acid, galacturonic acid, galactose and arabinose, with an average molecular weight of 830.93 kDa. FT-IR and NMR spectra of CAP2-1 exhibited typical characteristic signals of polysaccharide. For antioxidant activity evaluation in vitro, CAP2-1 showed effective scavenging capacities against ABTS, DPPH and superoxide anion radical in a dose-dependent manner, with IC50 values at 0.1232, 0.5543 and 0.5881 mg/mL, respectively. At cellular level, CAP2-1 provided a significant protective effect against hydrogen peroxide-induced oxidative stress in HepG2 cells by a compositive oxidation defense mechanism. CAP2-1 could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme SOD and non-enzymatic antioxidant GSH in oxidative damaged cells, in addition to scavenging ROS directly and improving cell viability and membrane integrity, consequently achieving the intracellular antioxidant activity. The results unveiled that CAP2-1 could be explored as a promising natural antioxidant for application in functional foods. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/30096394/Preparation_characterization_antioxidant_activity_and_protective_effect_against_cellular_oxidative_stress_of_polysaccharide_from_Cynanchum_auriculatum_Royle_ex_Wight_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(18)32205-0 DB - PRIME DP - Unbound Medicine ER -