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Evaluation of the Impacts of Long-Term Enriched Artemia with Bacillus subtilis on Growth Performance, Reproduction, Intestinal Microflora, and Resistance to Aeromonas hydrophila of Ornamental Fish Poecilia latipinna.
Probiotics Antimicrob Proteins. 2019 09; 11(3):957-965.PA

Abstract

The present study investigated the effect of enriched Artemia with Bacillus subtilis on growth performance, reproductive factors, proximate composition, intestinal microflora, and resistance to Aeromonas hydrophila of ornamental fish, Poecilia latipinna. Using a completely randomized design, the experiment included three groups. The first group was fed with commercial food without any probiotic. The second group was fed with unenriched Artemia, and the last group consumed long-time enriched Artemia with Bacillus subtilis. The bacteria B. subtilis with a density of 1 × 105 CFU mL-1 was added daily to Artemia culture medium. The total microflora and Bacillus subtilis counts were significantly increased in enriched Artemia compared to the unenriched group (P < 0.05). In fish fed groups, growth factors did not show any significant difference (P > 0.05). The maximum relative fecundity (28.65 ± 2.52 egg number g-1), fry production (62.93 ± 4.6 individual per female), and fry survival (70.97 ± 1.56%) obtained in the third group were found to be significantly more than those in the first and the second groups. Moreover, intestinal bacterial count for Bacillus revealed that the higher concentration of bacteria was significantly related to the third group (6.24 ± 0.11 log CFU g-1) (P < 0.05). Maximum protein and fat contents were observed in fish fed with Bacillus-enriched Artemia; however, no significant difference was found between control and unenriched Artemia groups (P > 0.05). The highest amount of ash was observed in fish fed with commercial food without any probiotic (P < 0.05). At the end of the feeding period, each of the three groups along with positive group (oxytetracycline 100 mg kg-1 of commercial food) was exposed to A. hydrophila (BCCM5/LMG3770) bacteria intraperitoneally. Based on the results, the lowest cumulative mortality was significantly found in group three (68.75 ± 3.6%) and positive group (62.5 ± 7.0%) compared to control and unenriched Artemia groups (P < 0.05). Hence, B. subtilis with a concentration of 1 × 105 CFU mL-1 during the period of Artemia culturing can improve the reproductive parameters, intestinal microflora, and resistance to pathogenic bacteria of Poecilia latipinna.

Authors+Show Affiliations

Department of Fisheries, Faculty of Natural Resources, Urmia University, P.O. Box: 46414-356, Urmia, West Azerbaijan, Iran. n.ahmadifard@urmia.ac.ir.Department of Fisheries, Faculty of Natural Resources, Urmia University, P.O. Box: 46414-356, Urmia, West Azerbaijan, Iran.Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azerbaijan, Iran.Department of Artemia, Urmia Lake Research Institute, Urmia University, Urmia, West Azerbaijan, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30097884

Citation

Ahmadifard, Nasrollah, et al. "Evaluation of the Impacts of Long-Term Enriched Artemia With Bacillus Subtilis On Growth Performance, Reproduction, Intestinal Microflora, and Resistance to Aeromonas Hydrophila of Ornamental Fish Poecilia Latipinna." Probiotics and Antimicrobial Proteins, vol. 11, no. 3, 2019, pp. 957-965.
Ahmadifard N, Rezaei Aminlooi V, Tukmechi A, et al. Evaluation of the Impacts of Long-Term Enriched Artemia with Bacillus subtilis on Growth Performance, Reproduction, Intestinal Microflora, and Resistance to Aeromonas hydrophila of Ornamental Fish Poecilia latipinna. Probiotics Antimicrob Proteins. 2019;11(3):957-965.
Ahmadifard, N., Rezaei Aminlooi, V., Tukmechi, A., & Agh, N. (2019). Evaluation of the Impacts of Long-Term Enriched Artemia with Bacillus subtilis on Growth Performance, Reproduction, Intestinal Microflora, and Resistance to Aeromonas hydrophila of Ornamental Fish Poecilia latipinna. Probiotics and Antimicrobial Proteins, 11(3), 957-965. https://doi.org/10.1007/s12602-018-9453-4
Ahmadifard N, et al. Evaluation of the Impacts of Long-Term Enriched Artemia With Bacillus Subtilis On Growth Performance, Reproduction, Intestinal Microflora, and Resistance to Aeromonas Hydrophila of Ornamental Fish Poecilia Latipinna. Probiotics Antimicrob Proteins. 2019;11(3):957-965. PubMed PMID: 30097884.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the Impacts of Long-Term Enriched Artemia with Bacillus subtilis on Growth Performance, Reproduction, Intestinal Microflora, and Resistance to Aeromonas hydrophila of Ornamental Fish Poecilia latipinna. AU - Ahmadifard,Nasrollah, AU - Rezaei Aminlooi,Vahid, AU - Tukmechi,Amir, AU - Agh,Naser, PY - 2018/8/12/pubmed PY - 2018/8/12/medline PY - 2018/8/12/entrez KW - Aeromonas hydrophila KW - Artemia KW - Bacillus subtilis KW - Poecilia latipinna KW - Probiotics KW - Reproduction SP - 957 EP - 965 JF - Probiotics and antimicrobial proteins JO - Probiotics Antimicrob Proteins VL - 11 IS - 3 N2 - The present study investigated the effect of enriched Artemia with Bacillus subtilis on growth performance, reproductive factors, proximate composition, intestinal microflora, and resistance to Aeromonas hydrophila of ornamental fish, Poecilia latipinna. Using a completely randomized design, the experiment included three groups. The first group was fed with commercial food without any probiotic. The second group was fed with unenriched Artemia, and the last group consumed long-time enriched Artemia with Bacillus subtilis. The bacteria B. subtilis with a density of 1 × 105 CFU mL-1 was added daily to Artemia culture medium. The total microflora and Bacillus subtilis counts were significantly increased in enriched Artemia compared to the unenriched group (P < 0.05). In fish fed groups, growth factors did not show any significant difference (P > 0.05). The maximum relative fecundity (28.65 ± 2.52 egg number g-1), fry production (62.93 ± 4.6 individual per female), and fry survival (70.97 ± 1.56%) obtained in the third group were found to be significantly more than those in the first and the second groups. Moreover, intestinal bacterial count for Bacillus revealed that the higher concentration of bacteria was significantly related to the third group (6.24 ± 0.11 log CFU g-1) (P < 0.05). Maximum protein and fat contents were observed in fish fed with Bacillus-enriched Artemia; however, no significant difference was found between control and unenriched Artemia groups (P > 0.05). The highest amount of ash was observed in fish fed with commercial food without any probiotic (P < 0.05). At the end of the feeding period, each of the three groups along with positive group (oxytetracycline 100 mg kg-1 of commercial food) was exposed to A. hydrophila (BCCM5/LMG3770) bacteria intraperitoneally. Based on the results, the lowest cumulative mortality was significantly found in group three (68.75 ± 3.6%) and positive group (62.5 ± 7.0%) compared to control and unenriched Artemia groups (P < 0.05). Hence, B. subtilis with a concentration of 1 × 105 CFU mL-1 during the period of Artemia culturing can improve the reproductive parameters, intestinal microflora, and resistance to pathogenic bacteria of Poecilia latipinna. SN - 1867-1314 UR - https://www.unboundmedicine.com/medline/citation/30097884/Evaluation_of_the_Impacts_of_Long_Term_Enriched_Artemia_with_Bacillus_subtilis_on_Growth_Performance_Reproduction_Intestinal_Microflora_and_Resistance_to_Aeromonas_hydrophila_of_Ornamental_Fish_Poecilia_latipinna_ L2 - https://dx.doi.org/10.1007/s12602-018-9453-4 DB - PRIME DP - Unbound Medicine ER -