TY - JOUR T1 - Functional and structural biomarkers to monitor heavy metal pollution of one of the most contaminated freshwater sites in Southern Europe. AU - Maresca,Viviana, AU - Fusaro,Lina, AU - Sorbo,Sergio, AU - Siciliano,Antonietta, AU - Loppi,Stefano, AU - Paoli,Luca, AU - Monaci,Fabrizio, AU - Karam,Elham Asadi, AU - Piscopo,Marina, AU - Guida,Marco, AU - Galdiero,Emilia, AU - Insolvibile,Marilena, AU - Basile,Adriana, Y1 - 2018/08/08/ PY - 2018/03/28/received PY - 2018/07/04/revised PY - 2018/07/30/accepted PY - 2018/8/12/pubmed PY - 2018/11/9/medline PY - 2018/8/12/entrez KW - Biomarkers KW - Biomonitoring KW - DNA damages KW - Heavy metal pollution KW - Leptodictyum riparium KW - Oxidative stress KW - Ultrastructure damage SP - 665 EP - 673 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 163 N2 - This study evaluated the biological effects of highly polluted freshwater environment (Regi Lagni channels, S Italy) on the aquatic moss Leptodictyum riparium, exposed in bags at three sites representative of different environmental conditions and characterized by different heavy metal burdens. Bioaccumulation, ultrastructural alterations, Reactive Oxygen Species (ROS) production, antioxidant enzymes activity and DNA damage were assessed. To better evaluate the biological response of the moss species to heavy metals, the same biological parameters were assessed also in L. riparium samples cultured in vitro using metal mixtures at the same concentrations as measured at the 3 field exposure sites. Heavy metals were accumulated into the moss tissues causing severe ultra-structural damages at higher concentration case studies, and the ROS production as well as the activity of the enzyme followed a concentration-dependent increase. However, the DNA damage trend suggested a threshold effect that changed between field and in vitro experiment. The enrichment factor suggests that the concentration in the most polluted site is close to the upper limit of L. riparium to accumulate metals. Overall, combining measures of the morpho-functional traits at different level contribute to improving the knowledge about the tolerance of L. riparium to heavy metal stress, suggesting that this moss could be suitable for biomonitoring activity in field conditions. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/30098556/Functional_and_structural_biomarkers_to_monitor_heavy_metal_pollution_of_one_of_the_most_contaminated_freshwater_sites_in_Southern_Europe_ DB - PRIME DP - Unbound Medicine ER -