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Cryo-EM structure of the SARS coronavirus spike glycoprotein in complex with its host cell receptor ACE2.
PLoS Pathog. 2018 Aug; 14(8):e1007236.PP

Abstract

The trimeric SARS coronavirus (SARS-CoV) surface spike (S) glycoprotein consisting of three S1-S2 heterodimers binds the cellular receptor angiotensin-converting enzyme 2 (ACE2) and mediates fusion of the viral and cellular membranes through a pre- to postfusion conformation transition. Here, we report the structure of the SARS-CoV S glycoprotein in complex with its host cell receptor ACE2 revealed by cryo-electron microscopy (cryo-EM). The complex structure shows that only one receptor-binding domain of the trimeric S glycoprotein binds ACE2 and adopts a protruding "up" conformation. In addition, we studied the structures of the SARS-CoV S glycoprotein and its complexes with ACE2 in different in vitro conditions, which may mimic different conformational states of the S glycoprotein during virus entry. Disassociation of the S1-ACE2 complex from some of the prefusion spikes was observed and characterized. We also characterized the rosette-like structures of the clustered SARS-CoV S2 trimers in the postfusion state observed on electron micrographs. Structural comparisons suggested that the SARS-CoV S glycoprotein retains a prefusion architecture after trypsin cleavage into the S1 and S2 subunits and acidic pH treatment. However, binding to the receptor opens up the receptor-binding domain of S1, which could promote the release of the S1-ACE2 complex and S1 monomers from the prefusion spike and trigger the pre- to postfusion conformational transition.

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Authors+Show Affiliations

Song W
The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University, Beijing, China.
Gui M
Center for Infectious Disease Research, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing Advanced Innovation Center for Structural Biology, Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China.
Wang X
The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University, Beijing, China. Collaborative Innovation Center for Biotherapy, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan, China.
Xiang Y
Center for Infectious Disease Research, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing Advanced Innovation Center for Structural Biology, Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China.

MeSH

Angiotensin-Converting Enzyme 2Cryoelectron MicroscopyModels, MolecularPeptidyl-Dipeptidase AProtein BindingProtein Interaction Domains and MotifsProtein MultimerizationProtein Structure, QuaternaryReceptors, VirusSevere acute respiratory syndrome-related coronavirusSevere Acute Respiratory SyndromeSpike Glycoprotein, CoronavirusVirus Internalization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30102747

Citation

Song, Wenfei, et al. "Cryo-EM Structure of the SARS Coronavirus Spike Glycoprotein in Complex With Its Host Cell Receptor ACE2." PLoS Pathogens, vol. 14, no. 8, 2018, pp. e1007236.
Song W, Gui M, Wang X, et al. Cryo-EM structure of the SARS coronavirus spike glycoprotein in complex with its host cell receptor ACE2. PLoS Pathog. 2018;14(8):e1007236.
Song, W., Gui, M., Wang, X., & Xiang, Y. (2018). Cryo-EM structure of the SARS coronavirus spike glycoprotein in complex with its host cell receptor ACE2. PLoS Pathogens, 14(8), e1007236. https://doi.org/10.1371/journal.ppat.1007236
Song W, et al. Cryo-EM Structure of the SARS Coronavirus Spike Glycoprotein in Complex With Its Host Cell Receptor ACE2. PLoS Pathog. 2018;14(8):e1007236. PubMed PMID: 30102747.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cryo-EM structure of the SARS coronavirus spike glycoprotein in complex with its host cell receptor ACE2. AU - Song,Wenfei, AU - Gui,Miao, AU - Wang,Xinquan, AU - Xiang,Ye, Y1 - 2018/08/13/ PY - 2018/2/15/received PY - 2018/7/23/accepted PY - 2018/8/23/revised PY - 2018/8/14/pubmed PY - 2019/1/23/medline PY - 2018/8/14/entrez SP - e1007236 EP - e1007236 JF - PLoS pathogens JO - PLoS Pathog VL - 14 IS - 8 N2 - The trimeric SARS coronavirus (SARS-CoV) surface spike (S) glycoprotein consisting of three S1-S2 heterodimers binds the cellular receptor angiotensin-converting enzyme 2 (ACE2) and mediates fusion of the viral and cellular membranes through a pre- to postfusion conformation transition. Here, we report the structure of the SARS-CoV S glycoprotein in complex with its host cell receptor ACE2 revealed by cryo-electron microscopy (cryo-EM). The complex structure shows that only one receptor-binding domain of the trimeric S glycoprotein binds ACE2 and adopts a protruding "up" conformation. In addition, we studied the structures of the SARS-CoV S glycoprotein and its complexes with ACE2 in different in vitro conditions, which may mimic different conformational states of the S glycoprotein during virus entry. Disassociation of the S1-ACE2 complex from some of the prefusion spikes was observed and characterized. We also characterized the rosette-like structures of the clustered SARS-CoV S2 trimers in the postfusion state observed on electron micrographs. Structural comparisons suggested that the SARS-CoV S glycoprotein retains a prefusion architecture after trypsin cleavage into the S1 and S2 subunits and acidic pH treatment. However, binding to the receptor opens up the receptor-binding domain of S1, which could promote the release of the S1-ACE2 complex and S1 monomers from the prefusion spike and trigger the pre- to postfusion conformational transition. SN - 1553-7374 UR - https://www.unboundmedicine.com/medline/citation/30102747/Cryo_EM_structure_of_the_SARS_coronavirus_spike_glycoprotein_in_complex_with_its_host_cell_receptor_ACE2_ DB - PRIME DP - Unbound Medicine ER -