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Survey for major viruses in commercial Vitis vinifera wine grapes in Ontario.
Virol J. 2018 08 13; 15(1):127.VJ

Abstract

BACKGROUND

In recent years, the Ontario grape and wine industry has experienced outbreaks of viral diseases across the province. Little is known about the prevalence of viruses and viral diseases in Ontario. Since 2015, we have conducted large-scale surveys for major viruses in commercial wine grapes in order to obtain a comprehensive understanding of the prevalence and severity of viral diseases in Ontario.

METHODS

A total of 657 composite leaf samples representing 3285 vines collected from 137 vine blocks of 33 vineyards from three appellations: Niagara Peninsula, Lake Erie North Shore and Prince Edward County. These samples covered six major red cultivars and five major white grape cultivars. Using a multiplex RT-PCR format, we tested these samples for 17 viruses including those involved in all major viral diseases of the grapevine, such as five grapevine leafroll-associated viruses (GLRaV-1, 2, 3, 4, 7), grapevine red blotch virus (GRBV), grapevine Pinot gris virus (GPGV), grapevine rupestris stem sitting-associated virus (GRSPaV), grapevine virus A (GVA), grapevine virus B (GVB), grapevine fleck virus (GFkV), arabis mosaic virus (ArMV), tomato ringspot virus (ToRSV), trapevine fanleaf virus (GFLV), among others.

RESULTS

Fourteen of the 17 viruses were detected from these samples and the predominant viruses are GRSPaV, GLRaV-3, GFkV, GPGV and GRBaV with an incidence of 84.0, 47.9, 21.8, 21.6 and 18.3%, respectively. As expected, mixed infections with multiple viruses are common. 95.6% of the samples included in the survey were infected with at least one virus; 67% of the samples with 2-4 viruses and 4.7% of the samples with 5-6 viruses. The major grape cultivars all tested positive for these major viruses. The results also suggested that the use of infected planting material may have been one of the chief factors responsible for the recent outbreaks of viral diseases across the province.

CONCLUSIONS

This is the first such comprehensive survey for grapevine viruses in Ontario and one of the most extensive surveys ever conducted in Canada. The recent outbreaks of viral diseases in Ontario vineyards were likely caused by GLRaV-3, GRBV and GPGV. Findings from this survey provides a baseline for the grape and wine industry in developing strategies for managing grapevine viral diseases in Ontario vineyards.

Authors+Show Affiliations

Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, ON, N1G 2W1, Canada.Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, ON, N1G 2W1, Canada.Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, ON, N1G 2W1, Canada.Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, ON, N1G 2W1, Canada.Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, ON, N1G 2W1, Canada. bmeng@uoguelph.ca.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30103767

Citation

Xiao, Huogen, et al. "Survey for Major Viruses in Commercial Vitis Vinifera Wine Grapes in Ontario." Virology Journal, vol. 15, no. 1, 2018, p. 127.
Xiao H, Shabanian M, Moore C, et al. Survey for major viruses in commercial Vitis vinifera wine grapes in Ontario. Virol J. 2018;15(1):127.
Xiao, H., Shabanian, M., Moore, C., Li, C., & Meng, B. (2018). Survey for major viruses in commercial Vitis vinifera wine grapes in Ontario. Virology Journal, 15(1), 127. https://doi.org/10.1186/s12985-018-1036-1
Xiao H, et al. Survey for Major Viruses in Commercial Vitis Vinifera Wine Grapes in Ontario. Virol J. 2018 08 13;15(1):127. PubMed PMID: 30103767.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Survey for major viruses in commercial Vitis vinifera wine grapes in Ontario. AU - Xiao,Huogen, AU - Shabanian,Mehdi, AU - Moore,Clayton, AU - Li,Caihong, AU - Meng,Baozhong, Y1 - 2018/08/13/ PY - 2018/05/29/received PY - 2018/07/31/accepted PY - 2018/8/15/entrez PY - 2018/8/15/pubmed PY - 2018/12/28/medline KW - Grapevine fleck KW - Grapevine leafroll KW - Grapevine red blotch KW - Grapevine rugose wood KW - Grapevine viral disease KW - Ontario KW - RT-PCR KW - Virus survey KW - Vitis vinifera SP - 127 EP - 127 JF - Virology journal JO - Virol J VL - 15 IS - 1 N2 - BACKGROUND: In recent years, the Ontario grape and wine industry has experienced outbreaks of viral diseases across the province. Little is known about the prevalence of viruses and viral diseases in Ontario. Since 2015, we have conducted large-scale surveys for major viruses in commercial wine grapes in order to obtain a comprehensive understanding of the prevalence and severity of viral diseases in Ontario. METHODS: A total of 657 composite leaf samples representing 3285 vines collected from 137 vine blocks of 33 vineyards from three appellations: Niagara Peninsula, Lake Erie North Shore and Prince Edward County. These samples covered six major red cultivars and five major white grape cultivars. Using a multiplex RT-PCR format, we tested these samples for 17 viruses including those involved in all major viral diseases of the grapevine, such as five grapevine leafroll-associated viruses (GLRaV-1, 2, 3, 4, 7), grapevine red blotch virus (GRBV), grapevine Pinot gris virus (GPGV), grapevine rupestris stem sitting-associated virus (GRSPaV), grapevine virus A (GVA), grapevine virus B (GVB), grapevine fleck virus (GFkV), arabis mosaic virus (ArMV), tomato ringspot virus (ToRSV), trapevine fanleaf virus (GFLV), among others. RESULTS: Fourteen of the 17 viruses were detected from these samples and the predominant viruses are GRSPaV, GLRaV-3, GFkV, GPGV and GRBaV with an incidence of 84.0, 47.9, 21.8, 21.6 and 18.3%, respectively. As expected, mixed infections with multiple viruses are common. 95.6% of the samples included in the survey were infected with at least one virus; 67% of the samples with 2-4 viruses and 4.7% of the samples with 5-6 viruses. The major grape cultivars all tested positive for these major viruses. The results also suggested that the use of infected planting material may have been one of the chief factors responsible for the recent outbreaks of viral diseases across the province. CONCLUSIONS: This is the first such comprehensive survey for grapevine viruses in Ontario and one of the most extensive surveys ever conducted in Canada. The recent outbreaks of viral diseases in Ontario vineyards were likely caused by GLRaV-3, GRBV and GPGV. Findings from this survey provides a baseline for the grape and wine industry in developing strategies for managing grapevine viral diseases in Ontario vineyards. SN - 1743-422X UR - https://www.unboundmedicine.com/medline/citation/30103767/Survey_for_major_viruses_in_commercial_Vitis_vinifera_wine_grapes_in_Ontario_ L2 - https://virologyj.biomedcentral.com/articles/10.1186/s12985-018-1036-1 DB - PRIME DP - Unbound Medicine ER -