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Optimization of total phenolic content from Terminalia chebula Retz. fruits using response surface methodology and evaluation of their antioxidant activities.
PLoS One. 2018; 13(8):e0202368.Plos

Abstract

Ultrasonic-assisted extraction (UAE), using aqueous ethanol as the solvent, was firstly applied to extract phenolic compounds from Terminalia chebula Retz. fruits (T. chebula fruits). In this study, ethanol concentration (%), ultrasonic intensity (W/cm2), particle diameter (mm), extraction temperature (°C), ultrasonic time (min), liquid-solid ratio (mL/g) and extraction cycle were investigated by single-factor experiment and then optimized using a Box-Behnken design. The optimized result for UAE was 68% ethanol concentration, ultrasonic intensity of 3.6 W/cm2, solid-liquid ratio of 23 mg/mL, particle size of 0.18 mm and ultrasonic time of 20 min for 2 times at 70 °C. The yield of total phenolic was 448.7 ± 2.15 mg GAE/g DW under the above optimum conditions, which agreed with the predicted value (447.8 mg GAE/g DW). Compared to conventional solvent extraction (CSE), UAE extracts showed excellent DPPH radical, DPPH, ABTS scavenging activities and reducing power in a dose-dependent manner, and better than that of CSE extracts. Additionally, the extract of the T. chebula fruits was analyzed by HPLC-ESI/MS. In summary, UAE could effectively extract phenolic compounds from T. chebula fruits. In addition, the extract could be used as a potential source of natural antioxidants.

Authors+Show Affiliations

College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30106980

Citation

Sheng, Zunlai, et al. "Optimization of Total Phenolic Content From Terminalia Chebula Retz. Fruits Using Response Surface Methodology and Evaluation of Their Antioxidant Activities." PloS One, vol. 13, no. 8, 2018, pp. e0202368.
Sheng Z, Zhao J, Muhammad I, et al. Optimization of total phenolic content from Terminalia chebula Retz. fruits using response surface methodology and evaluation of their antioxidant activities. PLoS ONE. 2018;13(8):e0202368.
Sheng, Z., Zhao, J., Muhammad, I., & Zhang, Y. (2018). Optimization of total phenolic content from Terminalia chebula Retz. fruits using response surface methodology and evaluation of their antioxidant activities. PloS One, 13(8), e0202368. https://doi.org/10.1371/journal.pone.0202368
Sheng Z, et al. Optimization of Total Phenolic Content From Terminalia Chebula Retz. Fruits Using Response Surface Methodology and Evaluation of Their Antioxidant Activities. PLoS ONE. 2018;13(8):e0202368. PubMed PMID: 30106980.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimization of total phenolic content from Terminalia chebula Retz. fruits using response surface methodology and evaluation of their antioxidant activities. AU - Sheng,Zunlai, AU - Zhao,Jiahong, AU - Muhammad,Ishfaq, AU - Zhang,Ying, Y1 - 2018/08/14/ PY - 2018/05/30/received PY - 2018/08/01/accepted PY - 2018/8/15/entrez PY - 2018/8/15/pubmed PY - 2019/2/5/medline SP - e0202368 EP - e0202368 JF - PloS one JO - PLoS ONE VL - 13 IS - 8 N2 - Ultrasonic-assisted extraction (UAE), using aqueous ethanol as the solvent, was firstly applied to extract phenolic compounds from Terminalia chebula Retz. fruits (T. chebula fruits). In this study, ethanol concentration (%), ultrasonic intensity (W/cm2), particle diameter (mm), extraction temperature (°C), ultrasonic time (min), liquid-solid ratio (mL/g) and extraction cycle were investigated by single-factor experiment and then optimized using a Box-Behnken design. The optimized result for UAE was 68% ethanol concentration, ultrasonic intensity of 3.6 W/cm2, solid-liquid ratio of 23 mg/mL, particle size of 0.18 mm and ultrasonic time of 20 min for 2 times at 70 °C. The yield of total phenolic was 448.7 ± 2.15 mg GAE/g DW under the above optimum conditions, which agreed with the predicted value (447.8 mg GAE/g DW). Compared to conventional solvent extraction (CSE), UAE extracts showed excellent DPPH radical, DPPH, ABTS scavenging activities and reducing power in a dose-dependent manner, and better than that of CSE extracts. Additionally, the extract of the T. chebula fruits was analyzed by HPLC-ESI/MS. In summary, UAE could effectively extract phenolic compounds from T. chebula fruits. In addition, the extract could be used as a potential source of natural antioxidants. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/30106980/Optimization_of_total_phenolic_content_from_Terminalia_chebula_Retz__fruits_using_response_surface_methodology_and_evaluation_of_their_antioxidant_activities_ L2 - http://dx.plos.org/10.1371/journal.pone.0202368 DB - PRIME DP - Unbound Medicine ER -