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Meiotic homoeologous recombination-based mapping of wheat chromosome 2B and its homoeologues in Aegilops speltoides and Thinopyrum elongatum.
Theor Appl Genet. 2018 Nov; 131(11):2381-2395.TA

Abstract

KEY MESSAGE

We physically dissected and mapped wheat chromosome 2B and its homoeologues in Aegilops speltoides and Thinopyrum elongatum based on meiotic homoeologous recombination, providing a unique physical framework for genome studies. Common wheat has a large and complex genome with narrow genetic diversity and various degrees of recombination between the A, B, and D subgenomes. This has limited the homologous recombination-based genome studies in wheat. Here, we exploited meiotic homoeologous recombination for molecular mapping of wheat chromosome 2B and its homoeologue 2S from Aegilops speltoides and 2E from Thinopyrum elongatum. The 2B-2S and 2B-2E recombination was induced by the ph1b mutant, and recovered using molecular markers and fluorescent genomic in situ hybridization (FGISH). A total of 112 2B-2S and 87 2B-2E recombinants involving different chromosome regions were developed and physically delineated by FGISH. The 2B-2S and 2B-2E recombination hotspots mapped to the subterminal regions on both arms. Recombination hotspots with the highest recombination rates mapped to the short arms. Eighty-three 2B-2S and 67 2B-2E recombinants were genotyped using the wheat 90 K SNP arrays. Based on the genotyping results and FGISH patterns of the recombinants, chromosomes 2B, 2S, and 2E were partitioned into 93, 66, and 46 bins, respectively. In total, 1037 SNPs physically mapped onto distinct bins of these three homoeologous chromosomes. A homoeologous recombination-based bin map was constructed for chromosome 2B, providing a unique physical framework for genome studies in wheat and its relatives. Meiotic homoeologous recombination also facilitates gene introgression to diversify the wheat genome for germplasm development. Therefore, homoeologous recombination-based studies enhance understanding of the wheat genome and its homoeologous counterparts from wild grasses, and expand the genetic variability of the wheat genome.

Authors+Show Affiliations

Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA.Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA.Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA.USDA-ARS, Red River Valley Agricultural Research Center, Fargo, ND, 58102, USA.USDA-ARS, Red River Valley Agricultural Research Center, Fargo, ND, 58102, USA.Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA. xiwen.cai@ndsu.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30109393

Citation

Zhang, Wei, et al. "Meiotic Homoeologous Recombination-based Mapping of Wheat Chromosome 2B and Its Homoeologues in Aegilops Speltoides and Thinopyrum Elongatum." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 131, no. 11, 2018, pp. 2381-2395.
Zhang W, Zhu X, Zhang M, et al. Meiotic homoeologous recombination-based mapping of wheat chromosome 2B and its homoeologues in Aegilops speltoides and Thinopyrum elongatum. Theor Appl Genet. 2018;131(11):2381-2395.
Zhang, W., Zhu, X., Zhang, M., Chao, S., Xu, S., & Cai, X. (2018). Meiotic homoeologous recombination-based mapping of wheat chromosome 2B and its homoeologues in Aegilops speltoides and Thinopyrum elongatum. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 131(11), 2381-2395. https://doi.org/10.1007/s00122-018-3160-0
Zhang W, et al. Meiotic Homoeologous Recombination-based Mapping of Wheat Chromosome 2B and Its Homoeologues in Aegilops Speltoides and Thinopyrum Elongatum. Theor Appl Genet. 2018;131(11):2381-2395. PubMed PMID: 30109393.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Meiotic homoeologous recombination-based mapping of wheat chromosome 2B and its homoeologues in Aegilops speltoides and Thinopyrum elongatum. AU - Zhang,Wei, AU - Zhu,Xianwen, AU - Zhang,Mingyi, AU - Chao,Shiaoman, AU - Xu,Steven, AU - Cai,Xiwen, Y1 - 2018/08/14/ PY - 2018/03/16/received PY - 2018/08/03/accepted PY - 2018/8/16/pubmed PY - 2018/10/23/medline PY - 2018/8/16/entrez SP - 2381 EP - 2395 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 131 IS - 11 N2 - KEY MESSAGE: We physically dissected and mapped wheat chromosome 2B and its homoeologues in Aegilops speltoides and Thinopyrum elongatum based on meiotic homoeologous recombination, providing a unique physical framework for genome studies. Common wheat has a large and complex genome with narrow genetic diversity and various degrees of recombination between the A, B, and D subgenomes. This has limited the homologous recombination-based genome studies in wheat. Here, we exploited meiotic homoeologous recombination for molecular mapping of wheat chromosome 2B and its homoeologue 2S from Aegilops speltoides and 2E from Thinopyrum elongatum. The 2B-2S and 2B-2E recombination was induced by the ph1b mutant, and recovered using molecular markers and fluorescent genomic in situ hybridization (FGISH). A total of 112 2B-2S and 87 2B-2E recombinants involving different chromosome regions were developed and physically delineated by FGISH. The 2B-2S and 2B-2E recombination hotspots mapped to the subterminal regions on both arms. Recombination hotspots with the highest recombination rates mapped to the short arms. Eighty-three 2B-2S and 67 2B-2E recombinants were genotyped using the wheat 90 K SNP arrays. Based on the genotyping results and FGISH patterns of the recombinants, chromosomes 2B, 2S, and 2E were partitioned into 93, 66, and 46 bins, respectively. In total, 1037 SNPs physically mapped onto distinct bins of these three homoeologous chromosomes. A homoeologous recombination-based bin map was constructed for chromosome 2B, providing a unique physical framework for genome studies in wheat and its relatives. Meiotic homoeologous recombination also facilitates gene introgression to diversify the wheat genome for germplasm development. Therefore, homoeologous recombination-based studies enhance understanding of the wheat genome and its homoeologous counterparts from wild grasses, and expand the genetic variability of the wheat genome. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/30109393/Meiotic_homoeologous_recombination_based_mapping_of_wheat_chromosome_2B_and_its_homoeologues_in_Aegilops_speltoides_and_Thinopyrum_elongatum_ L2 - https://dx.doi.org/10.1007/s00122-018-3160-0 DB - PRIME DP - Unbound Medicine ER -