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Altered anabolic signalling and reduced stimulation of myofibrillar protein synthesis after feeding and resistance exercise in people with obesity.
J Physiol. 2018 11; 596(21):5119-5133.JP

Abstract

KEY POINTS

Lifestyle modifications that include the regular performance of exercise are probably important for counteracting the negative consequences of obesity on postprandial myofibrillar protein synthetic responses to protein dense food ingestion. We show that the interactive effect of resistance exercise and feeding on the stimulation of myofibrillar protein synthesis rates is diminished with obesity compared to normal weight adults. The blunted myofibrillar protein synthetic response with resistance exercise in people with obesity may be underpinned by alterations in muscle anabolic signalling phosphorylation (p70S6K and 4E-BP1). The results obtained in the present study suggest that further exercise prescription manipulation may be necessary to optimize post-exercise myofibrillar protein synthesis rates in adults with obesity.

ABSTRACT

We aimed to determine whether obesity alters muscle anabolic and inflammatory signalling phosphorylation and also muscle protein synthesis within the myofibrillar (MYO) and sarcoplasmic (SARC) protein fractions after resistance exercise. Nine normal weight (NW) (21 ± 1 years, body mass index 22 ± 1 kg m-2) and nine obese (OB) (22 ± 1 years, body mass index 36 ± 2 kg m-2) adults received l-[ring-13 C6 ]phenylalanine infusions with blood and muscle sampling at basal and fed-state of the exercise (EX) and non-exercise (CON) legs. Participants performed unilateral leg extensions and consumed pork (36 g of protein) immediately after exercise. Basal muscle Toll-like receptor 4 (TLR4) protein was similar between OB and NW groups (P > 0.05) but increased at 300 min after pork ingestion only in the OB group (P = 0.03). Resistance exercise reduced TLR4 protein in the OB group at 300 min (EX vs. CON leg in OB: P = 0.04). Pork ingestion increased p70S6K phosphorylation at 300 min in CON and EX of the OB and NW groups (P > 0.05), although the response was lower in the EX leg of OB vs. NW at 300 min (P = 0.05). Basal MYO was similar between the NW and OB groups (P > 0.05) and was stimulated by pork ingestion in the EX and CON legs in both groups (Δ from basal NW: CON 0.04 ± 0.01% h-1 ; EX 0.10 ± 0.02% h-1 ; OB: CON 0.06 ± 0.01% h-1 ; EX 0.06 ± 0.01% h-1 ; P < 0.05). MYO was more strongly stimulated in the EX vs. CON legs in NW (P = 0.02) but not OB (P = 0.26). SARC was feeding sensitive but not further potentiated by resistance exercise in both groups. Our results suggest that obesity may attenuate the effectiveness of resistance exercise to augment fed-state MYO.

Authors+Show Affiliations

Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA.Department of Kinesiology and Community Health, University of Illinois at Urbana-Champaign, Urbana, IL, USA.Department of Kinesiology and Community Health, University of Illinois at Urbana-Champaign, Urbana, IL, USA.Department of Kinesiology and Community Health, University of Illinois at Urbana-Champaign, Urbana, IL, USA.Department of Kinesiology and Community Health, University of Illinois at Urbana-Champaign, Urbana, IL, USA.Department of Kinesiology and Community Health, University of Illinois at Urbana-Champaign, Urbana, IL, USA.Department of Kinesiology and Community Health, University of Illinois at Urbana-Champaign, Urbana, IL, USA.Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign, Urbana, IL, USA.Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign, Urbana, IL, USA.Department of Family Medicine, University of Illinois at Urbana-Champaign, Urbana, IL, USA.Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA. Department of Kinesiology and Community Health, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30113718

Citation

Beals, Joseph W., et al. "Altered Anabolic Signalling and Reduced Stimulation of Myofibrillar Protein Synthesis After Feeding and Resistance Exercise in People With Obesity." The Journal of Physiology, vol. 596, no. 21, 2018, pp. 5119-5133.
Beals JW, Skinner SK, McKenna CF, et al. Altered anabolic signalling and reduced stimulation of myofibrillar protein synthesis after feeding and resistance exercise in people with obesity. J Physiol. 2018;596(21):5119-5133.
Beals, J. W., Skinner, S. K., McKenna, C. F., Poozhikunnel, E. G., Farooqi, S. A., van Vliet, S., Martinez, I. G., Ulanov, A. V., Li, Z., Paluska, S. A., & Burd, N. A. (2018). Altered anabolic signalling and reduced stimulation of myofibrillar protein synthesis after feeding and resistance exercise in people with obesity. The Journal of Physiology, 596(21), 5119-5133. https://doi.org/10.1113/JP276210
Beals JW, et al. Altered Anabolic Signalling and Reduced Stimulation of Myofibrillar Protein Synthesis After Feeding and Resistance Exercise in People With Obesity. J Physiol. 2018;596(21):5119-5133. PubMed PMID: 30113718.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Altered anabolic signalling and reduced stimulation of myofibrillar protein synthesis after feeding and resistance exercise in people with obesity. AU - Beals,Joseph W, AU - Skinner,Sarah K, AU - McKenna,Colleen F, AU - Poozhikunnel,Elizabeth G, AU - Farooqi,Samee A, AU - van Vliet,Stephan, AU - Martinez,Isabel G, AU - Ulanov,Alexander V, AU - Li,Zhong, AU - Paluska,Scott A, AU - Burd,Nicholas A, Y1 - 2018/09/30/ PY - 2018/06/08/received PY - 2018/08/15/accepted PY - 2018/8/17/pubmed PY - 2019/11/5/medline PY - 2018/8/17/entrez KW - TLR4 KW - inflammation KW - leucine KW - mTORC1 KW - muscle mass KW - strength training SP - 5119 EP - 5133 JF - The Journal of physiology JO - J Physiol VL - 596 IS - 21 N2 - KEY POINTS: Lifestyle modifications that include the regular performance of exercise are probably important for counteracting the negative consequences of obesity on postprandial myofibrillar protein synthetic responses to protein dense food ingestion. We show that the interactive effect of resistance exercise and feeding on the stimulation of myofibrillar protein synthesis rates is diminished with obesity compared to normal weight adults. The blunted myofibrillar protein synthetic response with resistance exercise in people with obesity may be underpinned by alterations in muscle anabolic signalling phosphorylation (p70S6K and 4E-BP1). The results obtained in the present study suggest that further exercise prescription manipulation may be necessary to optimize post-exercise myofibrillar protein synthesis rates in adults with obesity. ABSTRACT: We aimed to determine whether obesity alters muscle anabolic and inflammatory signalling phosphorylation and also muscle protein synthesis within the myofibrillar (MYO) and sarcoplasmic (SARC) protein fractions after resistance exercise. Nine normal weight (NW) (21 ± 1 years, body mass index 22 ± 1 kg m-2) and nine obese (OB) (22 ± 1 years, body mass index 36 ± 2 kg m-2) adults received l-[ring-13 C6 ]phenylalanine infusions with blood and muscle sampling at basal and fed-state of the exercise (EX) and non-exercise (CON) legs. Participants performed unilateral leg extensions and consumed pork (36 g of protein) immediately after exercise. Basal muscle Toll-like receptor 4 (TLR4) protein was similar between OB and NW groups (P > 0.05) but increased at 300 min after pork ingestion only in the OB group (P = 0.03). Resistance exercise reduced TLR4 protein in the OB group at 300 min (EX vs. CON leg in OB: P = 0.04). Pork ingestion increased p70S6K phosphorylation at 300 min in CON and EX of the OB and NW groups (P > 0.05), although the response was lower in the EX leg of OB vs. NW at 300 min (P = 0.05). Basal MYO was similar between the NW and OB groups (P > 0.05) and was stimulated by pork ingestion in the EX and CON legs in both groups (Δ from basal NW: CON 0.04 ± 0.01% h-1 ; EX 0.10 ± 0.02% h-1 ; OB: CON 0.06 ± 0.01% h-1 ; EX 0.06 ± 0.01% h-1 ; P < 0.05). MYO was more strongly stimulated in the EX vs. CON legs in NW (P = 0.02) but not OB (P = 0.26). SARC was feeding sensitive but not further potentiated by resistance exercise in both groups. Our results suggest that obesity may attenuate the effectiveness of resistance exercise to augment fed-state MYO. SN - 1469-7793 UR - https://www.unboundmedicine.com/medline/citation/30113718/Altered_anabolic_signalling_and_reduced_stimulation_of_myofibrillar_protein_synthesis_after_feeding_and_resistance_exercise_in_people_with_obesity_ L2 - https://doi.org/10.1113/JP276210 DB - PRIME DP - Unbound Medicine ER -