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Are single-stranded circles intermediates in plasmid DNA replication?
EMBO J. 1986 Mar; 5(3):631-7.EJ

Abstract

Plasmid pC194 exists as circular double-stranded and single-stranded DNA in Bacillus subtilis and Staphylococcus aureus. We report here that the plasmid pHV33, composed of pBR322 and pC194, exists as double- and single-stranded DNA in Escherichia coli, provided that the replication functions of pC194 are intact. Single-stranded pHV33 DNA is converted to double-stranded DNA by complementary strand synthesis probably initiated at rriB, a primosome assembly site present on pBR322. The efficiency of complementary strand synthesis affects the double-stranded copy number, which suggests that single-stranded DNA is a plasmid replication intermediate.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3011418

Citation

te Riele, H, et al. "Are Single-stranded Circles Intermediates in Plasmid DNA Replication?" The EMBO Journal, vol. 5, no. 3, 1986, pp. 631-7.
te Riele H, Michel B, Ehrlich SD. Are single-stranded circles intermediates in plasmid DNA replication? EMBO J. 1986;5(3):631-7.
te Riele, H., Michel, B., & Ehrlich, S. D. (1986). Are single-stranded circles intermediates in plasmid DNA replication? The EMBO Journal, 5(3), 631-7.
te Riele H, Michel B, Ehrlich SD. Are Single-stranded Circles Intermediates in Plasmid DNA Replication. EMBO J. 1986;5(3):631-7. PubMed PMID: 3011418.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Are single-stranded circles intermediates in plasmid DNA replication? AU - te Riele,H, AU - Michel,B, AU - Ehrlich,S D, PY - 1986/3/1/pubmed PY - 1986/3/1/medline PY - 1986/3/1/entrez SP - 631 EP - 7 JF - The EMBO journal JO - EMBO J. VL - 5 IS - 3 N2 - Plasmid pC194 exists as circular double-stranded and single-stranded DNA in Bacillus subtilis and Staphylococcus aureus. We report here that the plasmid pHV33, composed of pBR322 and pC194, exists as double- and single-stranded DNA in Escherichia coli, provided that the replication functions of pC194 are intact. Single-stranded pHV33 DNA is converted to double-stranded DNA by complementary strand synthesis probably initiated at rriB, a primosome assembly site present on pBR322. The efficiency of complementary strand synthesis affects the double-stranded copy number, which suggests that single-stranded DNA is a plasmid replication intermediate. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/3011418/Are_single_stranded_circles_intermediates_in_plasmid_DNA_replication L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0261-4189&date=1986&volume=5&issue=3&spage=631 DB - PRIME DP - Unbound Medicine ER -