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Preparation and evaluation of a reversed-phase/hydrophilic interaction/ion-exchange mixed-mode chromatographic stationary phase functionalized with dopamine-based dendrimers.
J Chromatogr A 2018; 1571:165-175JC

Abstract

In this work, a novel dendritic stationary phase was synthesized by the repeated grafting of 1,4-butanediol diglycidyl ether (BDDE) and dopamine (DA) on the surface of silica for performing mixed-mode high-performance liquid chromatography (MHPLC). Elemental analysis (EA), thermogravimetric analysis (TGA) and Fourier transform infrared spectrometry (FT-IR) showed the successful preparation of the dendritic stationary phase. The prepared stationary phase showed the retention mechanisms of reversed-phase liquid chromatography (RPLC), hydrophilic interaction chromatography (HILIC) and ion-exchange chromatography (IEC) under different mobile phase conditions. In detail, alkylbenzenes, polycyclic aromatic hydrocarbons (PAHs) and hydrophobic positional isomers were separated successfully in the RPLC mode. The baseline separation of nucleobases, nucleosides and flavonoids was achieved under HILIC mode, respectively. Meanwhile, some acidic and basic analytes were used to evaluate the IEC mode. The effects of different chromatographic conditions, such as acetonitrile content, salt concentration and pH in the mobile phase, on the different chromatographic modes were also investigated. In addition, the application of the mixed-mode dendritic stationary phase was demonstrated by the analysis of traditional Chinese medicine (TCM), including Carthamus tinctorius L. and Abelmoschus manihot (Linn.) Medicus. Interestingly, the stationary phase also has the ability for the capture and separation of boric acids. These meaningful applications confirmed that the mixed-mode dendritic stationary phase can be potentially applied in the analysis of complex samples.

Authors+Show Affiliations

School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China.School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China.School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China.School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China.School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China.School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China.Analytical and Testing Center of Chongqing University, Chongqing, 401331, China.School of Pharmacy, Chongqing University, Chongqing, 401331, China.School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China. Electronic address: wlj@swmu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30115386

Citation

Zhou, Di, et al. "Preparation and Evaluation of a Reversed-phase/hydrophilic Interaction/ion-exchange Mixed-mode Chromatographic Stationary Phase Functionalized With Dopamine-based Dendrimers." Journal of Chromatography. A, vol. 1571, 2018, pp. 165-175.
Zhou D, Zeng J, Fu Q, et al. Preparation and evaluation of a reversed-phase/hydrophilic interaction/ion-exchange mixed-mode chromatographic stationary phase functionalized with dopamine-based dendrimers. J Chromatogr A. 2018;1571:165-175.
Zhou, D., Zeng, J., Fu, Q., Gao, D., Zhang, K., Ren, X., ... Wang, L. (2018). Preparation and evaluation of a reversed-phase/hydrophilic interaction/ion-exchange mixed-mode chromatographic stationary phase functionalized with dopamine-based dendrimers. Journal of Chromatography. A, 1571, pp. 165-175. doi:10.1016/j.chroma.2018.08.018.
Zhou D, et al. Preparation and Evaluation of a Reversed-phase/hydrophilic Interaction/ion-exchange Mixed-mode Chromatographic Stationary Phase Functionalized With Dopamine-based Dendrimers. J Chromatogr A. 2018 Oct 12;1571:165-175. PubMed PMID: 30115386.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation and evaluation of a reversed-phase/hydrophilic interaction/ion-exchange mixed-mode chromatographic stationary phase functionalized with dopamine-based dendrimers. AU - Zhou,Di, AU - Zeng,Jing, AU - Fu,Qifeng, AU - Gao,Die, AU - Zhang,Kailian, AU - Ren,Xiujun, AU - Zhou,Kai, AU - Xia,Zhining, AU - Wang,Lujun, Y1 - 2018/08/08/ PY - 2018/06/14/received PY - 2018/07/29/revised PY - 2018/08/07/accepted PY - 2018/8/18/pubmed PY - 2018/10/26/medline PY - 2018/8/18/entrez KW - Dendrimers KW - Dopamine KW - Mixed-mode stationary phase KW - Reversed-phase/hydrophilic interaction/ion exchange SP - 165 EP - 175 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1571 N2 - In this work, a novel dendritic stationary phase was synthesized by the repeated grafting of 1,4-butanediol diglycidyl ether (BDDE) and dopamine (DA) on the surface of silica for performing mixed-mode high-performance liquid chromatography (MHPLC). Elemental analysis (EA), thermogravimetric analysis (TGA) and Fourier transform infrared spectrometry (FT-IR) showed the successful preparation of the dendritic stationary phase. The prepared stationary phase showed the retention mechanisms of reversed-phase liquid chromatography (RPLC), hydrophilic interaction chromatography (HILIC) and ion-exchange chromatography (IEC) under different mobile phase conditions. In detail, alkylbenzenes, polycyclic aromatic hydrocarbons (PAHs) and hydrophobic positional isomers were separated successfully in the RPLC mode. The baseline separation of nucleobases, nucleosides and flavonoids was achieved under HILIC mode, respectively. Meanwhile, some acidic and basic analytes were used to evaluate the IEC mode. The effects of different chromatographic conditions, such as acetonitrile content, salt concentration and pH in the mobile phase, on the different chromatographic modes were also investigated. In addition, the application of the mixed-mode dendritic stationary phase was demonstrated by the analysis of traditional Chinese medicine (TCM), including Carthamus tinctorius L. and Abelmoschus manihot (Linn.) Medicus. Interestingly, the stationary phase also has the ability for the capture and separation of boric acids. These meaningful applications confirmed that the mixed-mode dendritic stationary phase can be potentially applied in the analysis of complex samples. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/30115386/Preparation_and_evaluation_of_a_reversed_phase/hydrophilic_interaction/ion_exchange_mixed_mode_chromatographic_stationary_phase_functionalized_with_dopamine_based_dendrimers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(18)31003-3 DB - PRIME DP - Unbound Medicine ER -