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Microevolution of epidemiological highly relevant non-O157 enterohemorrhagic Escherichia coli of serogroups O26 and O111.
Int J Med Microbiol. 2018 Dec; 308(8):1085-1095.IJ

Abstract

Enterohemorrhagic Escherichia coli (EHEC) are a cause of bloody diarrhea, hemorrhagic colitis (HC) and the potentially fatal hemolytic uremic syndrome (HUS). While O157:H7 is the dominant EHEC serotype, non-O157 EHEC have emerged as serious causes of disease. In Germany, the most important non-O157 O-serogroups causing one third of EHEC infections, including diarrhea as well as HUS, are O26, O103, O111 and O145. Interestingly, we identified EHEC O-serogroups O26 and O111 in one single sequence type complex, STC29, that also harbours atypical enteropathogenic E. coli (aEPEC). aEPEC differ from typical EHEC merely in the absence of stx-genes. These findings inspired us to unravel a putative microevolutionary scenario of these non-O157 EHEC by whole genome analyses. Analysis of single nucleotide polymorphisms (SNPs) of the maximum common genome (MCG) of 20 aEPEC (11 human/ 9 bovine) and 79 EHEC (42 human/ 36 bovine/ 1 food source) of STC29 identified three distinct clusters: Cluster 1 harboured strains of O-serogroup O111, the central Cluster 2 harboured only O26 aEPEC strains, while the more heterogeneous Cluster 3 contained both EHEC and aEPEC strains of O-serogroup O26. Further combined analyses of accessory virulence associated genes (VAGs) and insertion sites for mobile genetic elements suggested a parallel evolution of the MCG and the acquisition of virulence genes. The resulting microevolutionary model suggests the development of two distinct EHEC lineages from one common aEPEC ancestor of ST29 by lysogenic conversion with stx-converting bacteriophages, independent of the host species the strains had been isolated from. In conclusion, our cumulative data indicate that EHEC of O-serogroups O26 and O111 of STC29 originate from a common aEPEC ancestor and are bona fide zoonotic agents. The role of aEPEC in the emergence of O26 and O111 EHEC should be considered for infection control measures to prevent possible lysogenic conversion with stx-converting bacteriophages as major vehicle driving the emergence of EHEC lineages with direct Public Health consequences.

Authors+Show Affiliations

Institute of Microbiology and Epizootics, Freie Universität Berlin, Centre for Infection Medicine, Berlin, Germany.Robert Koch-Institute, Berlin, Germany.Institute of Hygiene, University Hospital Muenster, Muenster, Germany.Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom.Department of Bacteriology, Animal and Plant Health Agency, Weybridge, Addlestone, Surrey, United Kingdom.Robert Koch-Institute, Wernigerode, Germany.Institute of Hygiene, University Hospital Muenster, Muenster, Germany.Robert Koch-Institute, Berlin, Germany. Electronic address: WielerLH@rki.de.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30115547

Citation

Eichhorn, Inga, et al. "Microevolution of Epidemiological Highly Relevant non-O157 Enterohemorrhagic Escherichia Coli of Serogroups O26 and O111." International Journal of Medical Microbiology : IJMM, vol. 308, no. 8, 2018, pp. 1085-1095.
Eichhorn I, Semmler T, Mellmann A, et al. Microevolution of epidemiological highly relevant non-O157 enterohemorrhagic Escherichia coli of serogroups O26 and O111. Int J Med Microbiol. 2018;308(8):1085-1095.
Eichhorn, I., Semmler, T., Mellmann, A., Pickard, D., Anjum, M. F., Fruth, A., Karch, H., & Wieler, L. H. (2018). Microevolution of epidemiological highly relevant non-O157 enterohemorrhagic Escherichia coli of serogroups O26 and O111. International Journal of Medical Microbiology : IJMM, 308(8), 1085-1095. https://doi.org/10.1016/j.ijmm.2018.08.003
Eichhorn I, et al. Microevolution of Epidemiological Highly Relevant non-O157 Enterohemorrhagic Escherichia Coli of Serogroups O26 and O111. Int J Med Microbiol. 2018;308(8):1085-1095. PubMed PMID: 30115547.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microevolution of epidemiological highly relevant non-O157 enterohemorrhagic Escherichia coli of serogroups O26 and O111. AU - Eichhorn,Inga, AU - Semmler,Torsten, AU - Mellmann,Alexander, AU - Pickard,Derek, AU - Anjum,Muna F, AU - Fruth,Angelika, AU - Karch,Helge, AU - Wieler,Lothar H, Y1 - 2018/08/10/ PY - 2018/05/07/received PY - 2018/07/25/revised PY - 2018/08/08/accepted PY - 2018/8/18/pubmed PY - 2019/7/6/medline PY - 2018/8/18/entrez KW - Atypical enteropathogenic KW - Enterohemorrhagic KW - Escherichia coli (EHEC) KW - Escherichia coli (aEPEC) KW - Lysogenic conversion KW - Microevolutionary model KW - SNP-analysis KW - stx-converting bacteriophage SP - 1085 EP - 1095 JF - International journal of medical microbiology : IJMM JO - Int J Med Microbiol VL - 308 IS - 8 N2 - Enterohemorrhagic Escherichia coli (EHEC) are a cause of bloody diarrhea, hemorrhagic colitis (HC) and the potentially fatal hemolytic uremic syndrome (HUS). While O157:H7 is the dominant EHEC serotype, non-O157 EHEC have emerged as serious causes of disease. In Germany, the most important non-O157 O-serogroups causing one third of EHEC infections, including diarrhea as well as HUS, are O26, O103, O111 and O145. Interestingly, we identified EHEC O-serogroups O26 and O111 in one single sequence type complex, STC29, that also harbours atypical enteropathogenic E. coli (aEPEC). aEPEC differ from typical EHEC merely in the absence of stx-genes. These findings inspired us to unravel a putative microevolutionary scenario of these non-O157 EHEC by whole genome analyses. Analysis of single nucleotide polymorphisms (SNPs) of the maximum common genome (MCG) of 20 aEPEC (11 human/ 9 bovine) and 79 EHEC (42 human/ 36 bovine/ 1 food source) of STC29 identified three distinct clusters: Cluster 1 harboured strains of O-serogroup O111, the central Cluster 2 harboured only O26 aEPEC strains, while the more heterogeneous Cluster 3 contained both EHEC and aEPEC strains of O-serogroup O26. Further combined analyses of accessory virulence associated genes (VAGs) and insertion sites for mobile genetic elements suggested a parallel evolution of the MCG and the acquisition of virulence genes. The resulting microevolutionary model suggests the development of two distinct EHEC lineages from one common aEPEC ancestor of ST29 by lysogenic conversion with stx-converting bacteriophages, independent of the host species the strains had been isolated from. In conclusion, our cumulative data indicate that EHEC of O-serogroups O26 and O111 of STC29 originate from a common aEPEC ancestor and are bona fide zoonotic agents. The role of aEPEC in the emergence of O26 and O111 EHEC should be considered for infection control measures to prevent possible lysogenic conversion with stx-converting bacteriophages as major vehicle driving the emergence of EHEC lineages with direct Public Health consequences. SN - 1618-0607 UR - https://www.unboundmedicine.com/medline/citation/30115547/Microevolution_of_epidemiological_highly_relevant_non_O157_enterohemorrhagic_Escherichia_coli_of_serogroups_O26_and_O111_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1438-4221(18)30241-8 DB - PRIME DP - Unbound Medicine ER -