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Synthetic colloid fluids (6% hydroxyethyl starch 130/0.4 and 4% succinylated gelatin) interfere with total plasma protein measurements in vitro.
Vet Clin Pathol 2018; 47(4):575-581VC

Abstract

BACKGROUND

High molecular weight (MW) synthetic colloids interfere with refractometry. The effect of low MW synthetic colloids on refractometry is unknown.

OBJECTIVES

To investigate the effect of 6% hydroxyethyl starch 130/0.4 (HES) and 4% succinylated gelatin (GELO) on the refractometric measurement of total plasma protein (TPP) and colloid osmotic pressure (COP) in canine plasma.

METHODS

Heparinized plasma from 10 dogs was diluted with 0.9% saline (NaCl), HES, and GELO in ratios of plasma:fluid, 9:1, 8:2, 7:3, 6:4, and 5:5. The biuret TPP (TPPb), refractometric TPP (TPPr), and COP assays were performed for all dilutions. Agreement (bias, 95% limits of agreement (LOA)) was tested between the TPPb (NaCl) and the TPPr of the NaCl, HES, and GELO plasma dilutions, and between the TPPb (NaCl) and the TPPb of the HES and GELO plasma dilutions.

RESULTS

A small bias (0.06 g/dL, 95% LOA -0.51 to 0.39 g/dL) existed between TPPb (NaCl) and TPPr (NaCl). A large bias was found between TPPb (NaCl) and TPPr (HES) (1.38 g/dL, 95% LOA 0.30 to 3.10 g/dL), and TPPr (GELO) (1.18 g/dL, 95% LOA 0.53 to 2.88 g/dL). A small bias was found between TPPb (NaCl) and TPPb (HES) (0.08 g/dL, 95% LOA -2.04 to 2.20 g/dL), and a large bias was found between TPPb (NaCl) and TPPb (GELO) (1.00 g/dL, 95% LOA -0.59 to 2.60 g/dL). Increasing overestimation of TPPb (NaCl) by TPPr (HES and GELO) and TPPb (GELO) occurred with increasing colloid volumes.

CONCLUSIONS

HES and GELO interfered with TPPr, and GELO also interfered with TPPb, in vitro. These variables should be interpreted with caution for dogs that have received these synthetic colloids.

Authors+Show Affiliations

Comparative Health Research Group, School of Veterinary and Life Sciences, Murdoch University, Western Australia, Australia.Comparative Health Research Group, School of Veterinary and Life Sciences, Murdoch University, Western Australia, Australia.Comparative Health Research Group, School of Veterinary and Life Sciences, Murdoch University, Western Australia, Australia.Comparative Health Research Group, School of Veterinary and Life Sciences, Murdoch University, Western Australia, Australia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30117540

Citation

Yam, Elodie, et al. "Synthetic Colloid Fluids (6% Hydroxyethyl Starch 130/0.4 and 4% Succinylated Gelatin) Interfere With Total Plasma Protein Measurements in Vitro." Veterinary Clinical Pathology, vol. 47, no. 4, 2018, pp. 575-581.
Yam E, Hosgood G, Rossi G, et al. Synthetic colloid fluids (6% hydroxyethyl starch 130/0.4 and 4% succinylated gelatin) interfere with total plasma protein measurements in vitro. Vet Clin Pathol. 2018;47(4):575-581.
Yam, E., Hosgood, G., Rossi, G., & Smart, L. (2018). Synthetic colloid fluids (6% hydroxyethyl starch 130/0.4 and 4% succinylated gelatin) interfere with total plasma protein measurements in vitro. Veterinary Clinical Pathology, 47(4), pp. 575-581. doi:10.1111/vcp.12647.
Yam E, et al. Synthetic Colloid Fluids (6% Hydroxyethyl Starch 130/0.4 and 4% Succinylated Gelatin) Interfere With Total Plasma Protein Measurements in Vitro. Vet Clin Pathol. 2018;47(4):575-581. PubMed PMID: 30117540.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthetic colloid fluids (6% hydroxyethyl starch 130/0.4 and 4% succinylated gelatin) interfere with total plasma protein measurements in vitro. AU - Yam,Elodie, AU - Hosgood,Giselle, AU - Rossi,Gabriele, AU - Smart,Lisa, Y1 - 2018/08/17/ PY - 2017/11/14/received PY - 2018/03/02/revised PY - 2018/03/28/accepted PY - 2018/8/18/pubmed PY - 2019/1/18/medline PY - 2018/8/18/entrez KW - Biuret KW - fluid therapy KW - gelatin KW - hydroxyethyl starch KW - refractometry SP - 575 EP - 581 JF - Veterinary clinical pathology JO - Vet Clin Pathol VL - 47 IS - 4 N2 - BACKGROUND: High molecular weight (MW) synthetic colloids interfere with refractometry. The effect of low MW synthetic colloids on refractometry is unknown. OBJECTIVES: To investigate the effect of 6% hydroxyethyl starch 130/0.4 (HES) and 4% succinylated gelatin (GELO) on the refractometric measurement of total plasma protein (TPP) and colloid osmotic pressure (COP) in canine plasma. METHODS: Heparinized plasma from 10 dogs was diluted with 0.9% saline (NaCl), HES, and GELO in ratios of plasma:fluid, 9:1, 8:2, 7:3, 6:4, and 5:5. The biuret TPP (TPPb), refractometric TPP (TPPr), and COP assays were performed for all dilutions. Agreement (bias, 95% limits of agreement (LOA)) was tested between the TPPb (NaCl) and the TPPr of the NaCl, HES, and GELO plasma dilutions, and between the TPPb (NaCl) and the TPPb of the HES and GELO plasma dilutions. RESULTS: A small bias (0.06 g/dL, 95% LOA -0.51 to 0.39 g/dL) existed between TPPb (NaCl) and TPPr (NaCl). A large bias was found between TPPb (NaCl) and TPPr (HES) (1.38 g/dL, 95% LOA 0.30 to 3.10 g/dL), and TPPr (GELO) (1.18 g/dL, 95% LOA 0.53 to 2.88 g/dL). A small bias was found between TPPb (NaCl) and TPPb (HES) (0.08 g/dL, 95% LOA -2.04 to 2.20 g/dL), and a large bias was found between TPPb (NaCl) and TPPb (GELO) (1.00 g/dL, 95% LOA -0.59 to 2.60 g/dL). Increasing overestimation of TPPb (NaCl) by TPPr (HES and GELO) and TPPb (GELO) occurred with increasing colloid volumes. CONCLUSIONS: HES and GELO interfered with TPPr, and GELO also interfered with TPPb, in vitro. These variables should be interpreted with caution for dogs that have received these synthetic colloids. SN - 1939-165X UR - https://www.unboundmedicine.com/medline/citation/30117540/Synthetic_colloid_fluids__6_hydroxyethyl_starch_130/0_4_and_4_succinylated_gelatin__interfere_with_total_plasma_protein_measurements_in_vitro_ L2 - https://doi.org/10.1111/vcp.12647 DB - PRIME DP - Unbound Medicine ER -