Tags

Type your tag names separated by a space and hit enter

In vivo diffusion MRI detects early spinal cord axonal pathology in a mouse model of amyotrophic lateral sclerosis.
NMR Biomed. 2018 08; 31(8):e3954.NB

Abstract

Diffusion magnetic resonance imaging (MRI) exhibits contrast that identifies macro- and microstructural changes in neurodegenerative diseases. Previous studies have shown that MR diffusion tensor imaging (DTI) can observe changes in spinal cord white matter in animals and humans affected with symptomatic amyotrophic lateral sclerosis (ALS). The goal of this preclinical work was to investigate the sensitivity of DTI for the detection of signs of tissue damage before symptoms appear. High-field MRI data were acquired using a 9.4-T animal scanner to examine the spinal cord of an ALS mouse model at pre- and post-symptomatic stages (days 80 and 120, respectively). The MRI results were validated using yellow fluorescent protein (YFP) via optical microscopy of spinal cord tissue slices collected from the YFP,G93A-SOD1 mouse strain. DTI maps of diffusion-weighted imaging (DWI) signal intensity, mean diffusivity (MD), fractional anisotropy (FA), axial diffusivity (AD) and radial diffusivity (RD) were computed for axial slices of the lumbar region of the spinal cord. Significant changes were observed in FA (6.7% decrease, p < 0.01), AD (19.5% decrease, p < 0.01) and RD (16.1% increase, p < 0.001) at postnatal day 80 (P80). These differences were correlated with changes in axonal fluorescence intensity and membrane cellular markers. This study demonstrates the value of DTI as a potential tool to detect the underlying pathological progression associated with ALS, and may accelerate the discovery of therapeutic strategies for patients with this disease.

Authors+Show Affiliations

University of Illinois at Chicago, Anatomy and Cell Biology, Chicago, IL, USA.University of Illinois at Chicago, Bioengineering, Chicago, IL, USA.University of Illinois at Chicago, Bioengineering, Chicago, IL, USA.University of Illinois at Chicago, Bioengineering, Chicago, IL, USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30117615

Citation

Gatto, Rodolfo G., et al. "In Vivo Diffusion MRI Detects Early Spinal Cord Axonal Pathology in a Mouse Model of Amyotrophic Lateral Sclerosis." NMR in Biomedicine, vol. 31, no. 8, 2018, pp. e3954.
Gatto RG, Li W, Gao J, et al. In vivo diffusion MRI detects early spinal cord axonal pathology in a mouse model of amyotrophic lateral sclerosis. NMR Biomed. 2018;31(8):e3954.
Gatto, R. G., Li, W., Gao, J., & Magin, R. L. (2018). In vivo diffusion MRI detects early spinal cord axonal pathology in a mouse model of amyotrophic lateral sclerosis. NMR in Biomedicine, 31(8), e3954. https://doi.org/10.1002/nbm.3954
Gatto RG, et al. In Vivo Diffusion MRI Detects Early Spinal Cord Axonal Pathology in a Mouse Model of Amyotrophic Lateral Sclerosis. NMR Biomed. 2018;31(8):e3954. PubMed PMID: 30117615.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo diffusion MRI detects early spinal cord axonal pathology in a mouse model of amyotrophic lateral sclerosis. AU - Gatto,Rodolfo G, AU - Li,Weiguo, AU - Gao,Jin, AU - Magin,Richard L, Y1 - 2018/06/26/ PY - 2017/10/13/received PY - 2018/05/08/revised PY - 2018/05/09/accepted PY - 2018/8/18/entrez PY - 2018/8/18/pubmed PY - 2019/2/26/medline KW - G93A-SOD1 mice KW - MRI KW - amyotrophic lateral sclerosis KW - axonal degeneration KW - diffusion tensor imaging KW - spinal cord SP - e3954 EP - e3954 JF - NMR in biomedicine JO - NMR Biomed VL - 31 IS - 8 N2 - Diffusion magnetic resonance imaging (MRI) exhibits contrast that identifies macro- and microstructural changes in neurodegenerative diseases. Previous studies have shown that MR diffusion tensor imaging (DTI) can observe changes in spinal cord white matter in animals and humans affected with symptomatic amyotrophic lateral sclerosis (ALS). The goal of this preclinical work was to investigate the sensitivity of DTI for the detection of signs of tissue damage before symptoms appear. High-field MRI data were acquired using a 9.4-T animal scanner to examine the spinal cord of an ALS mouse model at pre- and post-symptomatic stages (days 80 and 120, respectively). The MRI results were validated using yellow fluorescent protein (YFP) via optical microscopy of spinal cord tissue slices collected from the YFP,G93A-SOD1 mouse strain. DTI maps of diffusion-weighted imaging (DWI) signal intensity, mean diffusivity (MD), fractional anisotropy (FA), axial diffusivity (AD) and radial diffusivity (RD) were computed for axial slices of the lumbar region of the spinal cord. Significant changes were observed in FA (6.7% decrease, p < 0.01), AD (19.5% decrease, p < 0.01) and RD (16.1% increase, p < 0.001) at postnatal day 80 (P80). These differences were correlated with changes in axonal fluorescence intensity and membrane cellular markers. This study demonstrates the value of DTI as a potential tool to detect the underlying pathological progression associated with ALS, and may accelerate the discovery of therapeutic strategies for patients with this disease. SN - 1099-1492 UR - https://www.unboundmedicine.com/medline/citation/30117615/In_vivo_diffusion_MRI_detects_early_spinal_cord_axonal_pathology_in_a_mouse_model_of_amyotrophic_lateral_sclerosis_ L2 - https://doi.org/10.1002/nbm.3954 DB - PRIME DP - Unbound Medicine ER -