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Confirmation of Syva enzyme multiple immunoassay technique (EMIT) d.a.u. and Roche Abuscreen radioimmunoassay (RIA) (125I) urine cannabinoid immunoassays by gas chromatographic/mass spectrometric (GC/MS) and bonded-phase adsorption/thin-layer chromatographic (BPA-TLC) methods.
J Forensic Sci. 1986 Apr; 31(2):494-500.JF

Abstract

Thirty human urines screened positive by the Syva enzyme multiple immunoassay technique (EMIT) d.a.u. urine cannabinoid assay were also positive for the major marijuana urinary metabolite 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) when assayed by gas chromatographic/mass spectrometric (GC/MS) and a noninstrumental qualitative bonded-phase adsorption/thin-layer chromatographic (BPA-TLC) technique. The noninstrumental BPA-TLC procedure was the simpler of the two techniques to perform and interpret. Assay of these same samples by the Roche Abuscreen radioimmunoassay (RIA) for cannabinoids (125I) revealed that reliance on the 100-ng/mL equivalent positive calibrator yielded a high incidence of false negative results (10 out of 30). The performance of these same 4 assays on 30 true negatives also was evaluated. All samples were negative for cannabinoids by EMIT and RIA, and for THC-COOH by BPA-TLC. GC/MS assay, however, detected spurious low levels of approximately 5-ng/mL THC-COOH in two instances. Because of this, a reliability level of 10 ng/mL was set for the routine quantitative confirmation of THC-COOH by the GC/MS method.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

3011956

Citation

Kogan, M J., et al. "Confirmation of Syva Enzyme Multiple Immunoassay Technique (EMIT) D.a.u. and Roche Abuscreen Radioimmunoassay (RIA) (125I) Urine Cannabinoid Immunoassays By Gas Chromatographic/mass Spectrometric (GC/MS) and Bonded-phase Adsorption/thin-layer Chromatographic (BPA-TLC) Methods." Journal of Forensic Sciences, vol. 31, no. 2, 1986, pp. 494-500.
Kogan MJ, Al Razi J, Pierson DJ, et al. Confirmation of Syva enzyme multiple immunoassay technique (EMIT) d.a.u. and Roche Abuscreen radioimmunoassay (RIA) (125I) urine cannabinoid immunoassays by gas chromatographic/mass spectrometric (GC/MS) and bonded-phase adsorption/thin-layer chromatographic (BPA-TLC) methods. J Forensic Sci. 1986;31(2):494-500.
Kogan, M. J., Al Razi, J., Pierson, D. J., & Willson, N. J. (1986). Confirmation of Syva enzyme multiple immunoassay technique (EMIT) d.a.u. and Roche Abuscreen radioimmunoassay (RIA) (125I) urine cannabinoid immunoassays by gas chromatographic/mass spectrometric (GC/MS) and bonded-phase adsorption/thin-layer chromatographic (BPA-TLC) methods. Journal of Forensic Sciences, 31(2), 494-500.
Kogan MJ, et al. Confirmation of Syva Enzyme Multiple Immunoassay Technique (EMIT) D.a.u. and Roche Abuscreen Radioimmunoassay (RIA) (125I) Urine Cannabinoid Immunoassays By Gas Chromatographic/mass Spectrometric (GC/MS) and Bonded-phase Adsorption/thin-layer Chromatographic (BPA-TLC) Methods. J Forensic Sci. 1986;31(2):494-500. PubMed PMID: 3011956.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Confirmation of Syva enzyme multiple immunoassay technique (EMIT) d.a.u. and Roche Abuscreen radioimmunoassay (RIA) (125I) urine cannabinoid immunoassays by gas chromatographic/mass spectrometric (GC/MS) and bonded-phase adsorption/thin-layer chromatographic (BPA-TLC) methods. AU - Kogan,M J, AU - Al Razi,J, AU - Pierson,D J, AU - Willson,N J, PY - 1986/4/1/pubmed PY - 1986/4/1/medline PY - 1986/4/1/entrez SP - 494 EP - 500 JF - Journal of forensic sciences JO - J Forensic Sci VL - 31 IS - 2 N2 - Thirty human urines screened positive by the Syva enzyme multiple immunoassay technique (EMIT) d.a.u. urine cannabinoid assay were also positive for the major marijuana urinary metabolite 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) when assayed by gas chromatographic/mass spectrometric (GC/MS) and a noninstrumental qualitative bonded-phase adsorption/thin-layer chromatographic (BPA-TLC) technique. The noninstrumental BPA-TLC procedure was the simpler of the two techniques to perform and interpret. Assay of these same samples by the Roche Abuscreen radioimmunoassay (RIA) for cannabinoids (125I) revealed that reliance on the 100-ng/mL equivalent positive calibrator yielded a high incidence of false negative results (10 out of 30). The performance of these same 4 assays on 30 true negatives also was evaluated. All samples were negative for cannabinoids by EMIT and RIA, and for THC-COOH by BPA-TLC. GC/MS assay, however, detected spurious low levels of approximately 5-ng/mL THC-COOH in two instances. Because of this, a reliability level of 10 ng/mL was set for the routine quantitative confirmation of THC-COOH by the GC/MS method. SN - 0022-1198 UR - https://www.unboundmedicine.com/medline/citation/3011956/Confirmation_of_Syva_enzyme_multiple_immunoassay_technique__EMIT__d_a_u__and_Roche_Abuscreen_radioimmunoassay__RIA___125I__urine_cannabinoid_immunoassays_by_gas_chromatographic/mass_spectrometric__GC/MS__and_bonded_phase_adsorption/thin_layer_chromatographic__BPA_TLC__methods_ DB - PRIME DP - Unbound Medicine ER -