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Herb-partitioned moxibustion alleviates colon injuries in ulcerative colitis rats.
World J Gastroenterol. 2018 Aug 14; 24(30):3384-3397.WJ

Abstract

AIM

To observe the effect of herb-partitioned moxibustion (HPM) on expression of colonic cytokines in ulcerative colitis (UC) rats.

METHODS

A UC rat model was established by protein immunization in combination with topical chemical stimulation. Rats in the HPM group (n = 8) received HPM at bilateral Tianshu (ST25) points. The gross injury and pathological scores of the colon were recorded. The expression profile of colonic cytokines was assayed using the protein microarray technique. Specific differential cytokines were selected and verified by ELISA. The corresponding UniProt Accessions of the differentially expressed cytokines were retrieved in the UniProt database. The pathways involved were analyzed with the help of the KEGG PATHWAY database. The DAVID database was used for functional cluster and pathway analysis.

RESULTS

HPM improved colon injuries in UC rats, manifested by accelerated repair of ulcers and alleviation of inflammation, and the gross injury and pathological scores both significantly decreased (P < 0.01). Fold change > 1.3 or < 0.77 was taken as the screening standard. There were 77 down-regulated and 9 up-regulated differentially expressed colonic cytokines in the HPM group compared with the model group, and expression of 20 differed significantly (P < 0.05). Twelve of the 20 significantly differentially expressed cytokines [β-catenin, interleukin-1 receptor 6 (IL-1R6), IL-1β, B7-1, nerve growth factor receptor, AMP-activated protein kinase-α1, neuropilin-2, orexin A, adipocyte differentiation-related protein, IL-2, Fas and FasL] were up-regulated in the model group (n = 3, compared with the normal group) but down-regulated in the HPM group (n = 3, compared with the model group). Functional cluster analysis showed that the differentially expressed colonic cytokines in the HPM group regulated apoptosis and protein phosphorylation. KEGG pathway analysis showed that 52 down-regulated and 7 up-regulated differentially expressed colonic cytokines in the HPM group had pathways. The pathways that interacted between the cytokines and their receptors accounted for the largest proportion (28 of the down-regulated and 5 of the up-regulated cytokines).

CONCLUSION

HPM promotes the repair of colon injuries in UC rats, which is related to the regulation of several abnormally expressed cytokines.

Authors+Show Affiliations

Laboratory of Acupuncture-moxibustion and Immunology, Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China.Department of Integrated Traditional Chinese Medicine and Western Medicine, North Branch of Huashan Hospital, Fudan University, Shanghai 201907, China.Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China.Laboratory of Acupuncture-moxibustion and Immunology, Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China.Yueyang Clinical Medicine School, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.Yueyang Clinical Medicine School, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.Yueyang Clinical Medicine School, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.Laboratory of Acupuncture-moxibustion and Immunology, Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China.Laboratory of Acupuncture-moxibustion and Immunology, Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China.Laboratory of Acupuncture-moxibustion and Immunology, Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30122878

Citation

Zhang, Dan, et al. "Herb-partitioned Moxibustion Alleviates Colon Injuries in Ulcerative Colitis Rats." World Journal of Gastroenterology, vol. 24, no. 30, 2018, pp. 3384-3397.
Zhang D, Ren YB, Wei K, et al. Herb-partitioned moxibustion alleviates colon injuries in ulcerative colitis rats. World J Gastroenterol. 2018;24(30):3384-3397.
Zhang, D., Ren, Y. B., Wei, K., Hong, J., Yang, Y. T., Wu, L. J., Zhang, J., Shi, Z., Wu, H. G., & Ma, X. P. (2018). Herb-partitioned moxibustion alleviates colon injuries in ulcerative colitis rats. World Journal of Gastroenterology, 24(30), 3384-3397. https://doi.org/10.3748/wjg.v24.i30.3384
Zhang D, et al. Herb-partitioned Moxibustion Alleviates Colon Injuries in Ulcerative Colitis Rats. World J Gastroenterol. 2018 Aug 14;24(30):3384-3397. PubMed PMID: 30122878.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Herb-partitioned moxibustion alleviates colon injuries in ulcerative colitis rats. AU - Zhang,Dan, AU - Ren,Yan-Bo, AU - Wei,Kai, AU - Hong,Jue, AU - Yang,Yan-Ting, AU - Wu,Li-Jie, AU - Zhang,Ji, AU - Shi,Zheng, AU - Wu,Huan-Gan, AU - Ma,Xiao-Peng, PY - 2018/05/09/received PY - 2018/06/22/revised PY - 2018/06/30/accepted PY - 2018/8/21/entrez PY - 2018/8/21/pubmed PY - 2018/11/10/medline KW - Cytokine expression profile KW - Herb-partitioned moxibustion KW - Protein microarray KW - Rats KW - Ulcerative colitis SP - 3384 EP - 3397 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 24 IS - 30 N2 - AIM: To observe the effect of herb-partitioned moxibustion (HPM) on expression of colonic cytokines in ulcerative colitis (UC) rats. METHODS: A UC rat model was established by protein immunization in combination with topical chemical stimulation. Rats in the HPM group (n = 8) received HPM at bilateral Tianshu (ST25) points. The gross injury and pathological scores of the colon were recorded. The expression profile of colonic cytokines was assayed using the protein microarray technique. Specific differential cytokines were selected and verified by ELISA. The corresponding UniProt Accessions of the differentially expressed cytokines were retrieved in the UniProt database. The pathways involved were analyzed with the help of the KEGG PATHWAY database. The DAVID database was used for functional cluster and pathway analysis. RESULTS: HPM improved colon injuries in UC rats, manifested by accelerated repair of ulcers and alleviation of inflammation, and the gross injury and pathological scores both significantly decreased (P < 0.01). Fold change > 1.3 or < 0.77 was taken as the screening standard. There were 77 down-regulated and 9 up-regulated differentially expressed colonic cytokines in the HPM group compared with the model group, and expression of 20 differed significantly (P < 0.05). Twelve of the 20 significantly differentially expressed cytokines [β-catenin, interleukin-1 receptor 6 (IL-1R6), IL-1β, B7-1, nerve growth factor receptor, AMP-activated protein kinase-α1, neuropilin-2, orexin A, adipocyte differentiation-related protein, IL-2, Fas and FasL] were up-regulated in the model group (n = 3, compared with the normal group) but down-regulated in the HPM group (n = 3, compared with the model group). Functional cluster analysis showed that the differentially expressed colonic cytokines in the HPM group regulated apoptosis and protein phosphorylation. KEGG pathway analysis showed that 52 down-regulated and 7 up-regulated differentially expressed colonic cytokines in the HPM group had pathways. The pathways that interacted between the cytokines and their receptors accounted for the largest proportion (28 of the down-regulated and 5 of the up-regulated cytokines). CONCLUSION: HPM promotes the repair of colon injuries in UC rats, which is related to the regulation of several abnormally expressed cytokines. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/30122878/Herb_partitioned_moxibustion_alleviates_colon_injuries_in_ulcerative_colitis_rats_ L2 - http://www.wjgnet.com/1007-9327/full/v24/i30/3384.htm DB - PRIME DP - Unbound Medicine ER -