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Activated Yes-Associated Protein Accelerates Cell Cycle, Inhibits Apoptosis, and Delays Senescence in Human Periodontal Ligament Stem Cells.
Int J Med Sci. 2018; 15(11):1241-1250.IJ

Abstract

Objectives:

To provide insight into the biological effects of activated Yes-associated protein (YAP) on the proliferation, apoptosis, and senescence of human periodontal ligament stem cells (h-PDLSCs).

Methods:

h-PDLSCs were isolated by the limiting dilution method, and their surface markers were quantified by flow cytometry. Enhanced green fluorescence protein (EGFP)-labeled lentiviral vector was used to activate YAP in h-PDLSCs, then qRT-PCR and Western blotting were used to evaluate the expression level of YAP. Immunofluorescence was used to detect the location of YAP in h-PDLSCs. The proliferation activity was detected by cell counting kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EdU), and the cell cycle was determined by flow cytometry. Apoptosis was analyzed by Annexin V-APC staining. Cell senescence was detected by β-galactosidase staining. Proteins in ERK, Bcl-2, and p53 signaling pathways were detected by Western blotting.

Results:

h-PDLSCs were isolated successfully and were positive for human mesenchymal stem cell surface markers. After YAP was activated by lentiviral vector, the mRNA and protein of YAP were highly expressed, and more YAP translocated into the nucleus. When YAP was overexpressed in h-PDLSCs, proliferation activity was improved; early and late apoptosis rates decreased (P<0.05); the proportion of cells in G2/M phases increased (P<0.05), while that in G0/G1 phase decreased (P<0.05); cellular senescence was delayed (P<0.01); the expression of P-MEK, P-ERK, P-P90RSK and P-Msk increased, while the expression of Bcl-2 family members (Bak, Bid and Bik) decreased.

Conclusions:

Activated YAP promotes proliferation, inhibits apoptosis, and delays senescence of h-PDLSCs. The Hippo-YAP signaling pathway can influence ERK and Bcl-2 signaling pathways.

Authors+Show Affiliations

School of Stomatology, Shandong University, Jinan, China. Shandong provincial key laboratory of oral tissue regeneration, Jinan, China.Department of Obstetrics and Gynecology, Qilu hospital of Shandong University, Jinan, China.School of Stomatology, Shandong University, Jinan, China. Shandong provincial key laboratory of oral tissue regeneration, Jinan, China.School of Stomatology, Shandong University, Jinan, China. Shandong provincial key laboratory of oral tissue regeneration, Jinan, China.School of Control Science and Engineering, Shandong University, Jinan, China.School of Stomatology, Shandong University, Jinan, China. Shandong provincial key laboratory of oral tissue regeneration, Jinan, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30123063

Citation

Jia, Linglu, et al. "Activated Yes-Associated Protein Accelerates Cell Cycle, Inhibits Apoptosis, and Delays Senescence in Human Periodontal Ligament Stem Cells." International Journal of Medical Sciences, vol. 15, no. 11, 2018, pp. 1241-1250.
Jia L, Gu W, Zhang Y, et al. Activated Yes-Associated Protein Accelerates Cell Cycle, Inhibits Apoptosis, and Delays Senescence in Human Periodontal Ligament Stem Cells. Int J Med Sci. 2018;15(11):1241-1250.
Jia, L., Gu, W., Zhang, Y., Jiang, B., Qiao, X., & Wen, Y. (2018). Activated Yes-Associated Protein Accelerates Cell Cycle, Inhibits Apoptosis, and Delays Senescence in Human Periodontal Ligament Stem Cells. International Journal of Medical Sciences, 15(11), 1241-1250. https://doi.org/10.7150/ijms.25115
Jia L, et al. Activated Yes-Associated Protein Accelerates Cell Cycle, Inhibits Apoptosis, and Delays Senescence in Human Periodontal Ligament Stem Cells. Int J Med Sci. 2018;15(11):1241-1250. PubMed PMID: 30123063.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activated Yes-Associated Protein Accelerates Cell Cycle, Inhibits Apoptosis, and Delays Senescence in Human Periodontal Ligament Stem Cells. AU - Jia,Linglu, AU - Gu,Weiting, AU - Zhang,Yunpeng, AU - Jiang,Baoqi, AU - Qiao,Xu, AU - Wen,Yong, Y1 - 2018/07/30/ PY - 2018/01/23/received PY - 2018/06/28/accepted PY - 2018/8/21/entrez PY - 2018/8/21/pubmed PY - 2019/1/8/medline KW - Apoptosis KW - Cell Cycle KW - Human Periodontal Ligament Stem Cells KW - Senescence KW - Yap SP - 1241 EP - 1250 JF - International journal of medical sciences JO - Int J Med Sci VL - 15 IS - 11 N2 - Objectives: To provide insight into the biological effects of activated Yes-associated protein (YAP) on the proliferation, apoptosis, and senescence of human periodontal ligament stem cells (h-PDLSCs). Methods: h-PDLSCs were isolated by the limiting dilution method, and their surface markers were quantified by flow cytometry. Enhanced green fluorescence protein (EGFP)-labeled lentiviral vector was used to activate YAP in h-PDLSCs, then qRT-PCR and Western blotting were used to evaluate the expression level of YAP. Immunofluorescence was used to detect the location of YAP in h-PDLSCs. The proliferation activity was detected by cell counting kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EdU), and the cell cycle was determined by flow cytometry. Apoptosis was analyzed by Annexin V-APC staining. Cell senescence was detected by β-galactosidase staining. Proteins in ERK, Bcl-2, and p53 signaling pathways were detected by Western blotting. Results: h-PDLSCs were isolated successfully and were positive for human mesenchymal stem cell surface markers. After YAP was activated by lentiviral vector, the mRNA and protein of YAP were highly expressed, and more YAP translocated into the nucleus. When YAP was overexpressed in h-PDLSCs, proliferation activity was improved; early and late apoptosis rates decreased (P<0.05); the proportion of cells in G2/M phases increased (P<0.05), while that in G0/G1 phase decreased (P<0.05); cellular senescence was delayed (P<0.01); the expression of P-MEK, P-ERK, P-P90RSK and P-Msk increased, while the expression of Bcl-2 family members (Bak, Bid and Bik) decreased. Conclusions: Activated YAP promotes proliferation, inhibits apoptosis, and delays senescence of h-PDLSCs. The Hippo-YAP signaling pathway can influence ERK and Bcl-2 signaling pathways. SN - 1449-1907 UR - https://www.unboundmedicine.com/medline/citation/30123063/Activated_Yes_Associated_Protein_Accelerates_Cell_Cycle_Inhibits_Apoptosis_and_Delays_Senescence_in_Human_Periodontal_Ligament_Stem_Cells_ L2 - http://www.medsci.org/v15p1241.htm DB - PRIME DP - Unbound Medicine ER -