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Physical mapping of the blue-grained gene from Thinopyrum ponticum chromosome 4Ag and development of blue-grain-related molecular markers and a FISH probe based on SLAF-seq technology.
Theor Appl Genet. 2018 Nov; 131(11):2359-2370.TA

Abstract

KEY MESSAGE

A Thinopyrum ponticum chromosome 4Ag physical map was constructed, the blue-grained gene was localized, and related specific markers and a FISH probe were developed by SLAF-seq. Decaploid Thinopyrum ponticum (2n = 10x = 70) serves as an important gene pool for wheat improvement. The wheat-Th. ponticum 4Ag (4D) disomic substitution line Blue 58, derived from a distant hybridization between Th. ponticum and common wheat (Triticum aestivum L.), bears blue coloration in the aleurone layer. To map the blue-grained gene, eight wheat-Th. ponticum 4Ag translocation lines with different chromosomal segment sizes were obtained from Blue 58 using 60Co-γ ray irradiation and were characterized using cytogenetic and molecular marker analysis. A small-segment blue-grained wheat translocation line L13, accounting for one-fifth of 4AgL, was obtained. A physical map of chromosome 4Ag was constructed containing 573 specific-locus amplified fragment sequencing (SLAF-seq) markers, including three bins with 223 markers on 4AgS and eight bins with 350 markers on 4AgL. The blue-grained gene in three blue-grained translocation lines L5, L9, and L13, was located on bin 4AgL-6 with FL 0.75-0.89. Moreover, 89 blue-grain-related molecular markers and one fluorescence in situ hybridization (FISH) probe, pThp12.19, were identified in this bin. The newly developed translocation lines and the molecular markers and FISH probe will facilitate the application of the Th. ponticum-origin blue-grained characteristic in wheat breeding.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Liu L
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Luo Q
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China. University of Chinese Academy of Sciences, Beijing, 100049, China.
Li H
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Li B
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Li Z
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China. zsli@genetics.ac.cn.
Zheng Q
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China. qzheng@genetics.ac.cn.

MeSH

Chromosomes, PlantGenetic MarkersIn Situ Hybridization, FluorescencePhysical Chromosome MappingPigmentationPlant BreedingPoaceaeTranslocation, GeneticTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30128741

Citation

Liu, Liqin, et al. "Physical Mapping of the Blue-grained Gene From Thinopyrum Ponticum Chromosome 4Ag and Development of Blue-grain-related Molecular Markers and a FISH Probe Based On SLAF-seq Technology." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 131, no. 11, 2018, pp. 2359-2370.
Liu L, Luo Q, Li H, et al. Physical mapping of the blue-grained gene from Thinopyrum ponticum chromosome 4Ag and development of blue-grain-related molecular markers and a FISH probe based on SLAF-seq technology. Theor Appl Genet. 2018;131(11):2359-2370.
Liu, L., Luo, Q., Li, H., Li, B., Li, Z., & Zheng, Q. (2018). Physical mapping of the blue-grained gene from Thinopyrum ponticum chromosome 4Ag and development of blue-grain-related molecular markers and a FISH probe based on SLAF-seq technology. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 131(11), 2359-2370. https://doi.org/10.1007/s00122-018-3158-7
Liu L, et al. Physical Mapping of the Blue-grained Gene From Thinopyrum Ponticum Chromosome 4Ag and Development of Blue-grain-related Molecular Markers and a FISH Probe Based On SLAF-seq Technology. Theor Appl Genet. 2018;131(11):2359-2370. PubMed PMID: 30128741.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Physical mapping of the blue-grained gene from Thinopyrum ponticum chromosome 4Ag and development of blue-grain-related molecular markers and a FISH probe based on SLAF-seq technology. AU - Liu,Liqin, AU - Luo,Qiaoling, AU - Li,Hongwei, AU - Li,Bin, AU - Li,Zhensheng, AU - Zheng,Qi, Y1 - 2018/08/20/ PY - 2018/03/20/received PY - 2018/08/03/accepted PY - 2018/8/22/pubmed PY - 2018/10/23/medline PY - 2018/8/22/entrez SP - 2359 EP - 2370 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 131 IS - 11 N2 - KEY MESSAGE: A Thinopyrum ponticum chromosome 4Ag physical map was constructed, the blue-grained gene was localized, and related specific markers and a FISH probe were developed by SLAF-seq. Decaploid Thinopyrum ponticum (2n = 10x = 70) serves as an important gene pool for wheat improvement. The wheat-Th. ponticum 4Ag (4D) disomic substitution line Blue 58, derived from a distant hybridization between Th. ponticum and common wheat (Triticum aestivum L.), bears blue coloration in the aleurone layer. To map the blue-grained gene, eight wheat-Th. ponticum 4Ag translocation lines with different chromosomal segment sizes were obtained from Blue 58 using 60Co-γ ray irradiation and were characterized using cytogenetic and molecular marker analysis. A small-segment blue-grained wheat translocation line L13, accounting for one-fifth of 4AgL, was obtained. A physical map of chromosome 4Ag was constructed containing 573 specific-locus amplified fragment sequencing (SLAF-seq) markers, including three bins with 223 markers on 4AgS and eight bins with 350 markers on 4AgL. The blue-grained gene in three blue-grained translocation lines L5, L9, and L13, was located on bin 4AgL-6 with FL 0.75-0.89. Moreover, 89 blue-grain-related molecular markers and one fluorescence in situ hybridization (FISH) probe, pThp12.19, were identified in this bin. The newly developed translocation lines and the molecular markers and FISH probe will facilitate the application of the Th. ponticum-origin blue-grained characteristic in wheat breeding. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/30128741/Physical_mapping_of_the_blue_grained_gene_from_Thinopyrum_ponticum_chromosome_4Ag_and_development_of_blue_grain_related_molecular_markers_and_a_FISH_probe_based_on_SLAF_seq_technology_ DB - PRIME DP - Unbound Medicine ER -