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Impact of soil salinity on the microbial structure of halophyte rhizosphere microbiome.
World J Microbiol Biotechnol. 2018 Aug 20; 34(9):136.WJ

Abstract

The rhizosphere microbiome plays a significant role in the life of plants in promoting plant survival under adverse conditions. However, limited information is available about microbial diversity in saline environments. In the current study, we compared the composition of the rhizosphere microbiomes of the halophytes Urochloa, Kochia, Salsola, and Atriplex living in moderate and high salinity environments (Khewra salt mines; Pakistan) with that of the non-halophyte Triticum. Soil microbiomes analysis using pyrosequencing of 16S rRNA gene indicated that Actinobacteria were dominant in saline soil samples whereas Proteobacteria predominated in non-saline soil samples. Firmicutes, Acidobacteria, Bacteriodetes and Thaumarchaeota were predominant phyla in saline and non-saline soils, whereas Cyanobacteria, Verrucomicrobia, Gemmatimonadetes and the unclassified WPS-2 were less abundant. Sequences from Euryarchaeota, Ignavibacteriae, and Nanohaloarchaeota were identified only from the rhizosphere of halophytes. Dominant halophilic bacteria and archaea identified in this study included Agrococcus, Armatimonadetes gp4, Halalkalicoccus, Haloferula and Halobacterium. Our analysis showed that increases in soil salinity correlated with significant differences in the alpha and beta diversity of the microbial communities across saline and non-saline soil samples. Having a complete inventory of the soil bacteria from different saline environments in Pakistan will help in the discovery of potential inoculants for crops growing on salt-affected land.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Mukhtar S
Department of Biological Sciences, Forman Christian College (A Chartered University), Ferozepur Road, Lahore, 54600, Pakistan.
Mirza BS
Department of Biology, Missouri State University, 901 S, National Ave, Springfield, MO, USA.
Mehnaz S
Department of Biological Sciences, Forman Christian College (A Chartered University), Ferozepur Road, Lahore, 54600, Pakistan.
Mirza MS
Environmental Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, Faisalabad, Pakistan.
Mclean J
Utah Water Research Laboratory, Department of Civil and Environmental Engineering, Utah State University, Logan, UT, 84322-8200, USA.
Malik KA
Department of Biological Sciences, Forman Christian College (A Chartered University), Ferozepur Road, Lahore, 54600, Pakistan. kausermalik@fccollege.edu.pk.

MeSH

ArchaeaBacteriaDNA, BacterialEcosystemMetagenomicsMicrobiotaPakistanPhylogenyPlant RootsRNA, Ribosomal, 16SRhizosphereSalinitySalt-Tolerant PlantsSoilSoil Microbiology

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30128756

Citation

Mukhtar, Salma, et al. "Impact of Soil Salinity On the Microbial Structure of Halophyte Rhizosphere Microbiome." World Journal of Microbiology & Biotechnology, vol. 34, no. 9, 2018, p. 136.
Mukhtar S, Mirza BS, Mehnaz S, et al. Impact of soil salinity on the microbial structure of halophyte rhizosphere microbiome. World J Microbiol Biotechnol. 2018;34(9):136.
Mukhtar, S., Mirza, B. S., Mehnaz, S., Mirza, M. S., Mclean, J., & Malik, K. A. (2018). Impact of soil salinity on the microbial structure of halophyte rhizosphere microbiome. World Journal of Microbiology & Biotechnology, 34(9), 136. https://doi.org/10.1007/s11274-018-2509-5
Mukhtar S, et al. Impact of Soil Salinity On the Microbial Structure of Halophyte Rhizosphere Microbiome. World J Microbiol Biotechnol. 2018 Aug 20;34(9):136. PubMed PMID: 30128756.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impact of soil salinity on the microbial structure of halophyte rhizosphere microbiome. AU - Mukhtar,Salma, AU - Mirza,Babur Saeed, AU - Mehnaz,Samina, AU - Mirza,Muhammad Sajjad, AU - Mclean,Joan, AU - Malik,Kauser Abdulla, Y1 - 2018/08/20/ PY - 2018/03/11/received PY - 2018/07/27/accepted PY - 2018/8/22/entrez PY - 2018/8/22/pubmed PY - 2018/10/3/medline KW - 16S rRNA gene KW - Haloarchaea KW - Halophilic bacteria KW - Pyrosequencing KW - Soil microbiome KW - Soil salinity SP - 136 EP - 136 JF - World journal of microbiology & biotechnology JO - World J Microbiol Biotechnol VL - 34 IS - 9 N2 - The rhizosphere microbiome plays a significant role in the life of plants in promoting plant survival under adverse conditions. However, limited information is available about microbial diversity in saline environments. In the current study, we compared the composition of the rhizosphere microbiomes of the halophytes Urochloa, Kochia, Salsola, and Atriplex living in moderate and high salinity environments (Khewra salt mines; Pakistan) with that of the non-halophyte Triticum. Soil microbiomes analysis using pyrosequencing of 16S rRNA gene indicated that Actinobacteria were dominant in saline soil samples whereas Proteobacteria predominated in non-saline soil samples. Firmicutes, Acidobacteria, Bacteriodetes and Thaumarchaeota were predominant phyla in saline and non-saline soils, whereas Cyanobacteria, Verrucomicrobia, Gemmatimonadetes and the unclassified WPS-2 were less abundant. Sequences from Euryarchaeota, Ignavibacteriae, and Nanohaloarchaeota were identified only from the rhizosphere of halophytes. Dominant halophilic bacteria and archaea identified in this study included Agrococcus, Armatimonadetes gp4, Halalkalicoccus, Haloferula and Halobacterium. Our analysis showed that increases in soil salinity correlated with significant differences in the alpha and beta diversity of the microbial communities across saline and non-saline soil samples. Having a complete inventory of the soil bacteria from different saline environments in Pakistan will help in the discovery of potential inoculants for crops growing on salt-affected land. SN - 1573-0972 UR - https://www.unboundmedicine.com/medline/citation/30128756/Impact_of_soil_salinity_on_the_microbial_structure_of_halophyte_rhizosphere_microbiome_ DB - PRIME DP - Unbound Medicine ER -