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Dietary L-Tryptophan Modulates the Structural and Functional Composition of the Intestinal Microbiome in Weaned Piglets.
Front Microbiol. 2018; 9:1736.FM

Abstract

Background:

Intestinal microbiota plays an important role in regulating metabolism, physiology, and immune response of the host. L-Tryptophan (Trp) are metabolized by several genera of bacteria. It remains largely unknown whether Trp can regulate the composition and diversity of the intestinal microbiota and contribute to intestinal homeostasis.

Methods:

A total of 126 weaning piglets were fed a corn- and soybean meal-based diet supplemented with 0, 0.2, or 0.4% Trp for 4 weeks. The intestinal microbiota was measured by using bacterial 16S rRNA gene-based high-throughput sequencing methods. Metabolites of Trp and short-chain fatty acids (SCFAs) in the hindgut were determined by high-performance liquid chromatography and gas chromatography, respectively. The mRNA levels for aromatic hydrocarbon receptor (AhR), tumor necrotic factor-α (TNF-α), interleukin-8 (IL-8), and protein abundances of tight junction proteins were determined.

Results:

Compared with the control group, Trp supplementation enhanced piglet growth performance and markedly altered the intestinal microbial composition as evidenced by enhanced alpha and beta diversity in the microbiome (P < 0.05). The abundances of Prevotella, Roseburia, and Succinivibrio genera were enriched, but those of Clostridium sensu stricto and Clostridium XI, opportunistic pathogens, were decreased with dietary Trp supplementation. Analysis of metabolic pathways indicated enhanced indole alkaloid biosynthesis and Trp metabolism, which was validated by elevated concentrations of 3-indoleacetic acid and indole in the intestinal contents of Trp-supplemented piglets (P < 0.05). These changes in Trp metabolites were correlated with activation of AhR and cytochrome p4501 A1 (CYP1A1) in cecum and colonic tissues, and with a decrease in the intestinal mucosal IL-8 mRNA level. Moreover, the protein abundances for zonula occluden (ZO)-1 and occludin were upregulated by Trp supplementation in colonic tissues.

Conclusion:

Dietary Trp supplementation altered intestinal microbial composition and diversity, improved intestinal mucosal barrier function, activated AhR signaling, and downregulated expression of inflammatory cytokines in the large intestine of weaned piglets. These results indicate a crosstalk between dietary Trp and intestine in nutrition, microbial metabolism, and mucosal immunity.

Authors+Show Affiliations

State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, China.State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, China.State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, China.State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, China.State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, China.State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, China.State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, China.Henan Yinfa Animal Husbandry Co., Xinzheng, China.State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, China. Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing, China.State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, China. Department of Animal Science, Texas A&M University, College Station, TX, United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30131777

Citation

Liang, Haiwei, et al. "Dietary L-Tryptophan Modulates the Structural and Functional Composition of the Intestinal Microbiome in Weaned Piglets." Frontiers in Microbiology, vol. 9, 2018, p. 1736.
Liang H, Dai Z, Liu N, et al. Dietary L-Tryptophan Modulates the Structural and Functional Composition of the Intestinal Microbiome in Weaned Piglets. Front Microbiol. 2018;9:1736.
Liang, H., Dai, Z., Liu, N., Ji, Y., Chen, J., Zhang, Y., Yang, Y., Li, J., Wu, Z., & Wu, G. (2018). Dietary L-Tryptophan Modulates the Structural and Functional Composition of the Intestinal Microbiome in Weaned Piglets. Frontiers in Microbiology, 9, 1736. https://doi.org/10.3389/fmicb.2018.01736
Liang H, et al. Dietary L-Tryptophan Modulates the Structural and Functional Composition of the Intestinal Microbiome in Weaned Piglets. Front Microbiol. 2018;9:1736. PubMed PMID: 30131777.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary L-Tryptophan Modulates the Structural and Functional Composition of the Intestinal Microbiome in Weaned Piglets. AU - Liang,Haiwei, AU - Dai,Zhaolai, AU - Liu,Ning, AU - Ji,Yun, AU - Chen,Jingqing, AU - Zhang,Yunchang, AU - Yang,Ying, AU - Li,Ju, AU - Wu,Zhenlong, AU - Wu,Guoyao, Y1 - 2018/08/07/ PY - 2018/04/08/received PY - 2018/07/11/accepted PY - 2018/8/23/entrez PY - 2018/8/23/pubmed PY - 2018/8/23/medline KW - AhR KW - IAA KW - L-tryptophan KW - SCFAs KW - indole KW - intestinal barrier function KW - microbiota KW - weaned piglets SP - 1736 EP - 1736 JF - Frontiers in microbiology JO - Front Microbiol VL - 9 N2 - Background: Intestinal microbiota plays an important role in regulating metabolism, physiology, and immune response of the host. L-Tryptophan (Trp) are metabolized by several genera of bacteria. It remains largely unknown whether Trp can regulate the composition and diversity of the intestinal microbiota and contribute to intestinal homeostasis. Methods: A total of 126 weaning piglets were fed a corn- and soybean meal-based diet supplemented with 0, 0.2, or 0.4% Trp for 4 weeks. The intestinal microbiota was measured by using bacterial 16S rRNA gene-based high-throughput sequencing methods. Metabolites of Trp and short-chain fatty acids (SCFAs) in the hindgut were determined by high-performance liquid chromatography and gas chromatography, respectively. The mRNA levels for aromatic hydrocarbon receptor (AhR), tumor necrotic factor-α (TNF-α), interleukin-8 (IL-8), and protein abundances of tight junction proteins were determined. Results: Compared with the control group, Trp supplementation enhanced piglet growth performance and markedly altered the intestinal microbial composition as evidenced by enhanced alpha and beta diversity in the microbiome (P < 0.05). The abundances of Prevotella, Roseburia, and Succinivibrio genera were enriched, but those of Clostridium sensu stricto and Clostridium XI, opportunistic pathogens, were decreased with dietary Trp supplementation. Analysis of metabolic pathways indicated enhanced indole alkaloid biosynthesis and Trp metabolism, which was validated by elevated concentrations of 3-indoleacetic acid and indole in the intestinal contents of Trp-supplemented piglets (P < 0.05). These changes in Trp metabolites were correlated with activation of AhR and cytochrome p4501 A1 (CYP1A1) in cecum and colonic tissues, and with a decrease in the intestinal mucosal IL-8 mRNA level. Moreover, the protein abundances for zonula occluden (ZO)-1 and occludin were upregulated by Trp supplementation in colonic tissues. Conclusion: Dietary Trp supplementation altered intestinal microbial composition and diversity, improved intestinal mucosal barrier function, activated AhR signaling, and downregulated expression of inflammatory cytokines in the large intestine of weaned piglets. These results indicate a crosstalk between dietary Trp and intestine in nutrition, microbial metabolism, and mucosal immunity. SN - 1664-302X UR - https://www.unboundmedicine.com/medline/citation/30131777/Dietary_L_Tryptophan_Modulates_the_Structural_and_Functional_Composition_of_the_Intestinal_Microbiome_in_Weaned_Piglets_ L2 - https://doi.org/10.3389/fmicb.2018.01736 DB - PRIME DP - Unbound Medicine ER -
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