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Detection and biochemical characterization of insecticide resistance in field populations of Asian citrus psyllid in Guangdong of China.
Sci Rep. 2018 08 22; 8(1):12587.SR

Abstract

The Asian citrus psyllid, Diaphorina citri Kuwayama, is one of the most damaging pests of citrus-producing regions throughout the world. The use of insecticides is the main strategy for controlling psyllid and has increased year by year. In this study, four field populations of D. citri were evaluated for resistance to nine different insecticides using the leaf-dip method. The results showed that the highest level of resistance for D. citri was found in imidacloprid with a resistance ratio of 15.12 in the Zengcheng population compared with the laboratory susceptible population. This was followed by chlorpyriphos (6.47), dinotefuran (6.16), thiamethoxam (6.04), lambda-cyhalothrin (4.78), and bifenthrin (4.16). Piperonyl butoxide (PBO) and triphenyl phosphate (TPP) showed significant synergism on imidacloprid effects in the Zengcheng population (3.84- and 2.46-fold, respectively). Nevertheless, diethyl maleate (DEM) had no synergism on imidacloprid. Biochemical enzyme assays suggested that general esterase, glutathione S-transferase and cytochrome P450 monooxygenase activities were higher in the field-collected populations than in the laboratory susceptible population. However, glutathione S-transferase may play a minor role in the resistance of adult D. citri to insecticides. At the molecular level, resistance of D. citri to imidacloprid is mainly related to the increased expression of CYP4C68 and CYP4G70 (>5-fold).

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Authors+Show Affiliations

Tian F
College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Mo X
College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Rizvi SAH
College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Li C
College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Zeng X
College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. zengxn@scau.edu.cn.

MeSH

AnimalsChinaChlorpyrifosCitrusGuanidinesHemipteraInsecticide ResistanceInsecticidesNeonicotinoidsNitrilesNitro CompoundsPyrethrinsThiamethoxam

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30135479

Citation

Tian, Fajun, et al. "Detection and Biochemical Characterization of Insecticide Resistance in Field Populations of Asian Citrus Psyllid in Guangdong of China." Scientific Reports, vol. 8, no. 1, 2018, p. 12587.
Tian F, Mo X, Rizvi SAH, et al. Detection and biochemical characterization of insecticide resistance in field populations of Asian citrus psyllid in Guangdong of China. Sci Rep. 2018;8(1):12587.
Tian, F., Mo, X., Rizvi, S. A. H., Li, C., & Zeng, X. (2018). Detection and biochemical characterization of insecticide resistance in field populations of Asian citrus psyllid in Guangdong of China. Scientific Reports, 8(1), 12587. https://doi.org/10.1038/s41598-018-30674-5
Tian F, et al. Detection and Biochemical Characterization of Insecticide Resistance in Field Populations of Asian Citrus Psyllid in Guangdong of China. Sci Rep. 2018 08 22;8(1):12587. PubMed PMID: 30135479.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection and biochemical characterization of insecticide resistance in field populations of Asian citrus psyllid in Guangdong of China. AU - Tian,Fajun, AU - Mo,Xiufang, AU - Rizvi,Syed Arif Hussain, AU - Li,Chaofeng, AU - Zeng,Xinnian, Y1 - 2018/08/22/ PY - 2017/09/25/received PY - 2018/07/30/accepted PY - 2018/8/24/entrez PY - 2018/8/24/pubmed PY - 2019/11/27/medline SP - 12587 EP - 12587 JF - Scientific reports JO - Sci Rep VL - 8 IS - 1 N2 - The Asian citrus psyllid, Diaphorina citri Kuwayama, is one of the most damaging pests of citrus-producing regions throughout the world. The use of insecticides is the main strategy for controlling psyllid and has increased year by year. In this study, four field populations of D. citri were evaluated for resistance to nine different insecticides using the leaf-dip method. The results showed that the highest level of resistance for D. citri was found in imidacloprid with a resistance ratio of 15.12 in the Zengcheng population compared with the laboratory susceptible population. This was followed by chlorpyriphos (6.47), dinotefuran (6.16), thiamethoxam (6.04), lambda-cyhalothrin (4.78), and bifenthrin (4.16). Piperonyl butoxide (PBO) and triphenyl phosphate (TPP) showed significant synergism on imidacloprid effects in the Zengcheng population (3.84- and 2.46-fold, respectively). Nevertheless, diethyl maleate (DEM) had no synergism on imidacloprid. Biochemical enzyme assays suggested that general esterase, glutathione S-transferase and cytochrome P450 monooxygenase activities were higher in the field-collected populations than in the laboratory susceptible population. However, glutathione S-transferase may play a minor role in the resistance of adult D. citri to insecticides. At the molecular level, resistance of D. citri to imidacloprid is mainly related to the increased expression of CYP4C68 and CYP4G70 (>5-fold). SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/30135479/Detection_and_biochemical_characterization_of_insecticide_resistance_in_field_populations_of_Asian_citrus_psyllid_in_Guangdong_of_China_ DB - PRIME DP - Unbound Medicine ER -
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