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Junin virus isolation from lympho-mononuclear cells of patients with Argentine hemorrhagic fever.
Intervirology. 1986; 25(2):97-102.I

Abstract

Detection of viremia was attempted by three different methods in 30 cases of Argentine hemorrhagic fever (AHF). Cocultivation of peripheral blood mononuclear cells (PBMC) with Vero cell monolayers was the most sensitive, detecting Junin virus (JV) in 96% of the cases. Inoculation of whole blood into suckling mice and on Vero cells rendered 53 and 46% of positive isolations, respectively. The results presented suggest that PBMC are infected with JV during the acute period of AHF. JV was isolated with decreasing frequency up to 3 days after treatment with immune plasma, but no virus was recovered from PBMC during early convalescence.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3013799

Citation

Ambrosio, A M., et al. "Junin Virus Isolation From Lympho-mononuclear Cells of Patients With Argentine Hemorrhagic Fever." Intervirology, vol. 25, no. 2, 1986, pp. 97-102.
Ambrosio AM, Enria DA, Maiztegui JI. Junin virus isolation from lympho-mononuclear cells of patients with Argentine hemorrhagic fever. Intervirology. 1986;25(2):97-102.
Ambrosio, A. M., Enria, D. A., & Maiztegui, J. I. (1986). Junin virus isolation from lympho-mononuclear cells of patients with Argentine hemorrhagic fever. Intervirology, 25(2), 97-102.
Ambrosio AM, Enria DA, Maiztegui JI. Junin Virus Isolation From Lympho-mononuclear Cells of Patients With Argentine Hemorrhagic Fever. Intervirology. 1986;25(2):97-102. PubMed PMID: 3013799.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Junin virus isolation from lympho-mononuclear cells of patients with Argentine hemorrhagic fever. AU - Ambrosio,A M, AU - Enria,D A, AU - Maiztegui,J I, PY - 1986/1/1/pubmed PY - 1986/1/1/medline PY - 1986/1/1/entrez SP - 97 EP - 102 JF - Intervirology JO - Intervirology VL - 25 IS - 2 N2 - Detection of viremia was attempted by three different methods in 30 cases of Argentine hemorrhagic fever (AHF). Cocultivation of peripheral blood mononuclear cells (PBMC) with Vero cell monolayers was the most sensitive, detecting Junin virus (JV) in 96% of the cases. Inoculation of whole blood into suckling mice and on Vero cells rendered 53 and 46% of positive isolations, respectively. The results presented suggest that PBMC are infected with JV during the acute period of AHF. JV was isolated with decreasing frequency up to 3 days after treatment with immune plasma, but no virus was recovered from PBMC during early convalescence. SN - 0300-5526 UR - https://www.unboundmedicine.com/medline/citation/3013799/Junin_virus_isolation_from_lympho_mononuclear_cells_of_patients_with_Argentine_hemorrhagic_fever_ L2 - https://www.karger.com?DOI=10.1159/000149662 DB - PRIME DP - Unbound Medicine ER -