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Electrochemiluminescent aptasensor for thrombin using nitrogen-doped graphene quantum dots.
Mikrochim Acta. 2018 08 24; 185(9):430.MA

Abstract

An electrochemiluminescent (ECL) aptamer based method is described for the determination of thrombin. Three-dimensional nitrogen-doped graphene oxide (3D-NGO) was placed on a glassy carbon electrode (GCE) to provide an electrode surface that displays excellent electrical conductivity and acts as a strong emitter of ECL. The modified electrode was further coated with chitosan via electrodeposition. Finally, the amino-modified aptamer was immobilized on the modified GCE. The interaction between thrombin and aptamer results in a decrease in ECL. The assay has a linear response in the 1 fM to 1 nM thrombin concentration range and a 0.25 fM lower detection limit (at an S/N ratio of 3). The method was applied to the determination of thrombin in spiked human plasma samples, and recoveries ranged between 94 and 105% (with RSDs of <3.6%). The calibration plot was recorded at potential and wavelength of fluorescence emission (wavelength: 445 nm; potential: 0 to -2 V). Graphical abstract A bare glassy carbon electrode (GCE) does not display electrochemiluminescence (ECL). If, however, nitrogen-doped graphene quantum dots, chitosan, and three-dimensional nitrogen-doped graphene oxide (NGQD-chitosan/3D-NGO) are electrodeposited on the GCE, strong ECL can be observed. The ECL intensity decreased after aptamer and bovine serum albumin (BSA) were dropped onto the electrode (curve a). However, the ECL further decreases after addition of thrombin (TB; curve b).

Authors+Show Affiliations

State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2, Linggong Road, Ganjingzi District, Dalian, 116023, China.Key Laboratory of Xinjiang Endemic Phytomedicine Resources, Ministry of Education, School of Pharmacy, Shihezi University, Shihezi, 832000, China. sunsg@nwsuaf.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30143874

Citation

Khonsari, Yasamin Nasiri, and Shiguo Sun. "Electrochemiluminescent Aptasensor for Thrombin Using Nitrogen-doped Graphene Quantum Dots." Mikrochimica Acta, vol. 185, no. 9, 2018, p. 430.
Khonsari YN, Sun S. Electrochemiluminescent aptasensor for thrombin using nitrogen-doped graphene quantum dots. Mikrochim Acta. 2018;185(9):430.
Khonsari, Y. N., & Sun, S. (2018). Electrochemiluminescent aptasensor for thrombin using nitrogen-doped graphene quantum dots. Mikrochimica Acta, 185(9), 430. https://doi.org/10.1007/s00604-018-2942-z
Khonsari YN, Sun S. Electrochemiluminescent Aptasensor for Thrombin Using Nitrogen-doped Graphene Quantum Dots. Mikrochim Acta. 2018 08 24;185(9):430. PubMed PMID: 30143874.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemiluminescent aptasensor for thrombin using nitrogen-doped graphene quantum dots. AU - Khonsari,Yasamin Nasiri, AU - Sun,Shiguo, Y1 - 2018/08/24/ PY - 2018/04/17/received PY - 2018/08/02/accepted PY - 2018/8/26/entrez PY - 2018/8/26/pubmed PY - 2019/2/5/medline KW - Amino-modified aptamer KW - Aptamer KW - Chitosan KW - Electrochemiluminescence KW - Glassy carbon electrode KW - Human plasma samples KW - Modified electrode KW - Quantum dots KW - Three-dimensional nitrogen-doped graphene oxide SP - 430 EP - 430 JF - Mikrochimica acta JO - Mikrochim Acta VL - 185 IS - 9 N2 - An electrochemiluminescent (ECL) aptamer based method is described for the determination of thrombin. Three-dimensional nitrogen-doped graphene oxide (3D-NGO) was placed on a glassy carbon electrode (GCE) to provide an electrode surface that displays excellent electrical conductivity and acts as a strong emitter of ECL. The modified electrode was further coated with chitosan via electrodeposition. Finally, the amino-modified aptamer was immobilized on the modified GCE. The interaction between thrombin and aptamer results in a decrease in ECL. The assay has a linear response in the 1 fM to 1 nM thrombin concentration range and a 0.25 fM lower detection limit (at an S/N ratio of 3). The method was applied to the determination of thrombin in spiked human plasma samples, and recoveries ranged between 94 and 105% (with RSDs of <3.6%). The calibration plot was recorded at potential and wavelength of fluorescence emission (wavelength: 445 nm; potential: 0 to -2 V). Graphical abstract A bare glassy carbon electrode (GCE) does not display electrochemiluminescence (ECL). If, however, nitrogen-doped graphene quantum dots, chitosan, and three-dimensional nitrogen-doped graphene oxide (NGQD-chitosan/3D-NGO) are electrodeposited on the GCE, strong ECL can be observed. The ECL intensity decreased after aptamer and bovine serum albumin (BSA) were dropped onto the electrode (curve a). However, the ECL further decreases after addition of thrombin (TB; curve b). SN - 1436-5073 UR - https://www.unboundmedicine.com/medline/citation/30143874/Electrochemiluminescent_aptasensor_for_thrombin_using_nitrogen_doped_graphene_quantum_dots_ DB - PRIME DP - Unbound Medicine ER -