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Simultaneous quantification of THC-COOH, OH-THC, and further cannabinoids in human hair by gas chromatography-tandem mass spectrometry with electron ionization applying automated sample preparation.
Drug Test Anal. 2019 Feb; 11(2):267-278.DT

Abstract

The detection of Δ9 -tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN) in hair, for the purpose of identifying cannabis consumption, is conducted in many forensic laboratories. Since external contamination of hair with these cannabis components cannot be excluded, even after hair decontamination, only the detection of THC metabolites such as 11-nor-9-carboxy-Δ9 -tetrahydrocannabinol (THC-COOH) or 11-hydroxy-Δ9 -tetrahydrocannabinol (OH-THC), is considered to prove cannabis consumption. At present, testing for THC metabolites is not standard practice due to its analytical complexity. For these reasons, we developed a novel method for the detection of THC-COOH and OH-THC as well as THC, CBD, and CBN in one single analytical run using gas chromatography-tandem mass spectrometry (GC-MS/MS) with electron ionization. After manual hair washing and grinding, sample preparation was fully automated, by means of a robotic autosampler. The hair extraction took place by digestion with sodium hydroxide. A solid-phase extraction (SPE) was chosen for sample clean-up, using a mixed-mode anion exchange sorbent. Derivatization of all analytes was by silylation. The method has been fully validated according to guidelines of the Society of Toxicological and Forensic Chemistry (GTFCh), with a limit of detection (LOD) of 0.2 pg/mg for THC-COOH and OH-THC and 2 pg/mg for THC, CBD and CBN, respectively, thus fulfilling the Society of Hair Testing (SoHT) recommendations. The validated method has been successfully applied to our routine forensic case work and a summary of data from authentic hair samples is given, as well as data from proficiency tests.

Authors+Show Affiliations

Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Cologne, Germany.GERSTEL GmbH & Co. KG, Mülheim, Germany.Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Cologne, Germany.Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Cologne, Germany.Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Cologne, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30144294

Citation

Kieliba, Tobias, et al. "Simultaneous Quantification of THC-COOH, OH-THC, and Further Cannabinoids in Human Hair By Gas Chromatography-tandem Mass Spectrometry With Electron Ionization Applying Automated Sample Preparation." Drug Testing and Analysis, vol. 11, no. 2, 2019, pp. 267-278.
Kieliba T, Lerch O, Andresen-Streichert H, et al. Simultaneous quantification of THC-COOH, OH-THC, and further cannabinoids in human hair by gas chromatography-tandem mass spectrometry with electron ionization applying automated sample preparation. Drug Test Anal. 2019;11(2):267-278.
Kieliba, T., Lerch, O., Andresen-Streichert, H., Rothschild, M. A., & Beike, J. (2019). Simultaneous quantification of THC-COOH, OH-THC, and further cannabinoids in human hair by gas chromatography-tandem mass spectrometry with electron ionization applying automated sample preparation. Drug Testing and Analysis, 11(2), 267-278. https://doi.org/10.1002/dta.2490
Kieliba T, et al. Simultaneous Quantification of THC-COOH, OH-THC, and Further Cannabinoids in Human Hair By Gas Chromatography-tandem Mass Spectrometry With Electron Ionization Applying Automated Sample Preparation. Drug Test Anal. 2019;11(2):267-278. PubMed PMID: 30144294.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous quantification of THC-COOH, OH-THC, and further cannabinoids in human hair by gas chromatography-tandem mass spectrometry with electron ionization applying automated sample preparation. AU - Kieliba,Tobias, AU - Lerch,Oliver, AU - Andresen-Streichert,Hilke, AU - Rothschild,Markus A, AU - Beike,Justus, Y1 - 2018/09/25/ PY - 2018/05/14/received PY - 2018/08/13/revised PY - 2018/08/14/accepted PY - 2018/8/26/pubmed PY - 2019/7/2/medline PY - 2018/8/26/entrez KW - GC-EI-MS/MS KW - automated solid-phase extraction KW - hair analysis KW - proving cannabis consumption SP - 267 EP - 278 JF - Drug testing and analysis JO - Drug Test Anal VL - 11 IS - 2 N2 - The detection of Δ9 -tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN) in hair, for the purpose of identifying cannabis consumption, is conducted in many forensic laboratories. Since external contamination of hair with these cannabis components cannot be excluded, even after hair decontamination, only the detection of THC metabolites such as 11-nor-9-carboxy-Δ9 -tetrahydrocannabinol (THC-COOH) or 11-hydroxy-Δ9 -tetrahydrocannabinol (OH-THC), is considered to prove cannabis consumption. At present, testing for THC metabolites is not standard practice due to its analytical complexity. For these reasons, we developed a novel method for the detection of THC-COOH and OH-THC as well as THC, CBD, and CBN in one single analytical run using gas chromatography-tandem mass spectrometry (GC-MS/MS) with electron ionization. After manual hair washing and grinding, sample preparation was fully automated, by means of a robotic autosampler. The hair extraction took place by digestion with sodium hydroxide. A solid-phase extraction (SPE) was chosen for sample clean-up, using a mixed-mode anion exchange sorbent. Derivatization of all analytes was by silylation. The method has been fully validated according to guidelines of the Society of Toxicological and Forensic Chemistry (GTFCh), with a limit of detection (LOD) of 0.2 pg/mg for THC-COOH and OH-THC and 2 pg/mg for THC, CBD and CBN, respectively, thus fulfilling the Society of Hair Testing (SoHT) recommendations. The validated method has been successfully applied to our routine forensic case work and a summary of data from authentic hair samples is given, as well as data from proficiency tests. SN - 1942-7611 UR - https://www.unboundmedicine.com/medline/citation/30144294/Simultaneous_quantification_of_THC_COOH_OH_THC_and_further_cannabinoids_in_human_hair_by_gas_chromatography_tandem_mass_spectrometry_with_electron_ionization_applying_automated_sample_preparation_ L2 - https://doi.org/10.1002/dta.2490 DB - PRIME DP - Unbound Medicine ER -